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The map label for this gene is yddV [H]

Identifier: 218704961

GI number: 218704961

Start: 1763714

End: 1764757

Strand: Reverse

Name: yddV [H]

Synonym: ECUMN_1744

Alternate gene names: 218704961

Gene position: 1764757-1763714 (Counterclockwise)

Preceding gene: 218704962

Following gene: 218704960

Centisome position: 33.92

GC content: 43.3

Gene sequence:

>1044_bases
GTGCTGAAAAAAATCCTCTATCCAGTCATCTTCTCTTCGGATTATTCCGCCGCAGAAAAACTTCAGGTCTACCATTTCTC
TATTAACAGTATTGATATCGCTATGGAAGTGATGACCCGCGCGTTTACCTTTAGTGACAGTAGTGCCTCAAAGGAAGATG
AAAACTATCGTATCTTCTCGTTACTGGAAAATGCCGAAGAAGAAAAAGAGCGGCAAATAGCCTCATTACTTTCATGGGAA
ATAGATATTATCTATAAAGTCCTGCTGGATTCTGATTTAGGCAGTAGTTTGCCTTTAAGCCAGGCTGATTTTGGCCTGTG
GTTTAACCATAAAGGTCGACATTATTTTAGCGGCATAGCCGAAGTGGGCCATATCTCCCGTCTGATTCAGGATTTCGACG
GTATTTTCAATCAAACCATGCGTAACACCAGGAATTTGAATAACAGAAGTTTACGGGTAAAATTTTTATTACAGATAAGA
AATACCGTATCGCAAATTATTACCTTGCTGCGTGAATTGTTTGAAGAAGTATCGCGCCACGAAGTCGGTATGGATGTACT
GACGAAATTACTTAACCGCCGTTTCCTGCCGACGATCTTCAAACGCGAAATTGCCCATGCCAACCGGACCGGTACACCGC
TGTCAGTGCTGATTATTGACGTTGATAAATTCAAAGAGATTAACGATACGTGGGGTCATAACACTGGCGATGAAATTCTG
CGTAAAATCTCTCAGGCCTTTTATGACAACGTCCGCAGTAGTGATTATGTTTTCCGCTACGGGGGCGATGAATTTATCAT
TGTTTTGACTGAAGCTTCGGAAAACGAAACGTTACGTACCGCAGAACGTATTCGCAGTCGGGTGGAGAAAACCAAACTGA
AAGCCGCAAACGGCGAAGATATTGCCCTCTCACTTTCCATCGGTGCCGCCATGTTTAATGGTCATCCTGACTATGAGCGC
CTCATTCAAATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTGAACTCTGGAAAGCCAGTCT
TTAG

Upstream 100 bases:

>100_bases
GGTTGACGATGTCGAAAGGCTAATACAAATCCAGCATACCGTCGCGGAAGTGCATGCCCGCATAGGAATTCCGGTAGAAA
TTGTCGAGATGGGGTTTCGG

Downstream 100 bases:

>100_bases
ATGCGCCAGGATGCAGAGGTAATCATGAAGCTAACCGATGCGGATAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGA
GCAAAATATGATGGGCGCGG

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEEEKERQIASLLSWE
IDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIR
NTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEIL
RKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYER
LIQIADEALYIAKRRGRNRVELWKASL

Sequences:

>Translated_347_residues
MLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEEEKERQIASLLSWE
IDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIR
NTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEIL
RKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYER
LIQIADEALYIAKRRGRNRVELWKASL
>Mature_347_residues
MLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEEEKERQIASLLSWE
IDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIR
NTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEIL
RKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYER
LIQIADEALYIAKRRGRNRVELWKASL

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=347, Percent_Identity=98.8472622478386, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=34.5911949685535, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=240, Percent_Identity=30, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=35.2941176470588, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=31.0526315789474, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=33.1360946745562, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=35.1851851851852, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=153, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=32.484076433121, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=31.3513513513514, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=33.587786259542, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40029; Mature: 40029

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFS
CCHHHHHHHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLENAEEEKERQIASLLSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
EVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEIL
HCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHH
RKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGED
HHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
EEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFS
CCHHHHHHHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLENAEEEKERQIASLLSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
EVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEIL
HCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHH
RKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGED
HHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
EEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]