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The map label for this gene is dos [H]

Identifier: 218704960

GI number: 218704960

Start: 1761290

End: 1763689

Strand: Reverse

Name: dos [H]

Synonym: ECUMN_1743

Alternate gene names: 218704960

Gene position: 1763689-1761290 (Counterclockwise)

Preceding gene: 218704961

Following gene: 218704959

Centisome position: 33.9

GC content: 50.25

Gene sequence:

>2400_bases
ATGAAGCTAACCGATGCGGATAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGAGCAAAATATGATGGGCGCGGTGTT
AATTAACGAAAATGATGAAGTGATGTTTTTCAACCCCGCCGCAGAGAAGCTCTGGGGGTACAAACGTGAAGAAGTTATTG
GCAATAACATTGATATGCTGATTCCGCGGGATTTGCGTCCCGCGCATCCTGAATACATCCGTCACAACCGTGAAGGCGGT
AAAGCGCGCGTCGAGGGGATGAGTCGGGAGCTGCAGCTGGAGAAAAAAGACGGCAGTAAAATCTGGACCCGTTTTGCGCT
ATCGAAAGTGAGCGCCGAGGGGAAAGTTTATTACCTGGCGCTGGTACGGGATGCCAGCGTAGAAATGGCGCAAAAAGAAC
AGACCCGACAATTGATTATTGCCGTTGACCATCTCGACCGACCAGTGATTGTCCTCGATCCGGAACGCCATATTGTGCAG
TGCAATCGTGCATTTACCGAAATGTTTGGTTACTGCATTAGCGAAGCCAGCGGTATGCAGCCCGATACACTCCTGAACAT
TCCAGAATTCCCTGCTGATAACCGCATTCGTTTACAACAGTTGCTATGGAAAACCGCCCGCGATCAGGACGAATTTCTGC
TGTTGACGCGCACCGGTGAAAAAATCTGGATTAAAGCCTCTATCAGCCCGGTCTATGATGTGCTCGCGCATCTGCAGAAC
CTGGTAATGACTTTCTCGGATATCACCGAAGAACGGCAAATCCGCCAGCTTGAAGGCAATATTCTCGCCGCCATGTGCAG
CAGCCCGCCATTTCATGAAATGGGGGAAATTATTTGTCGTAACATCGAATCTGTACTCAACGAATCGCATGTTTCGCTGT
TCGCGCTGCGCAACGGGATGCCGCTACACTGGGCGTCATCTTCGCACGGTGCAGAAGTTCAAAATGCGCAAAGCTGGTCA
GCGACCATTCGTCAGCGTGATGGTGCGCCTGCGGGGATCCTGCAAATTAAAACCTCGTCAGGAGCAGAAACCAGCGCCTT
TATCGAACGCGTGGCAGATATCAGCCAGCATATGGCCGCGCTGGCGCTGGAACAGGAAAAAAGCCGTCAGCATATTGAAC
AACTCATCCAATTTGATCCGATGACCGGTCTGCCAAATCGCAATAACCTGCACAATTACCTCGATGACCTGGTCGACAAA
GCCGTCTCTCCGGTGGTGTATCTCATCGGTGTTGACCATATTCAGGATGTGATTGATAGCCTTGGCTATGCGTGGGCCGA
TCAGGCATTGCTGGAAGTGGTCAATCGCTTTCGTGAAAAACTCAAACCGGATCAGTATCTCTGTCGTATCGAAGGTACGC
AGTTTGTCCTCGTGAGCCTGGAAAACGACGTCAGTAACATAACCCAAATCGCCGATGAGCTACGGAATGTGGTCAGCAAG
CCGATAATGATTGACGATAAACCATTCCCGCTCACCTTGAGCATTGGCATCAGCTACGACGTGGGTAAAAACCGCGATTA
CCTGCTCTCCACTGCTCACAATGCAATGGATTATATTCGCAAGAATGGCGGTAACGGCTGGCAGTTCTTCAGCCCGGCGA
TGAACGAAATGGTAAAAGAGCGTTTGGTTTTAGGCGCGGCGCTGAAAGAAGCGATTAGCAATAACCAACTAAAACTGGTT
TACCAGCCGCAAATCTTCGCAGAAACGGGTGAGCTCTACGGCATCGAAGCCCTTGCTCGCTGGCACGATCCCCTGCATGG
TCATGTGCCCCCTTCACGGTTTATTCCTCTCGCAGAAGAGATCGGTGAAATCGAAAATATTGGACGCTGGGTCATCGCGG
AAGCTTGTCGTCAGTTAGCAGAATGGCGTAGCCAGAATATTCATATCCCGGCGTTATCCGTTAACTTGTCGGCGTTGCAC
TTTCGCAGTAATCAGCTGCCTAATCAGGTGTCTGATGCAATGCAAGCCTGGGGTATTGACGGCCACCAGCTGACGGTGGA
AATCACGGAAAGCATGATGATGGAACACGATACCGAAATCTTTAAGCGCATTCAGATCCTGCGCGATATGGGCGTAGGTT
TATCTGTCGATGATTTCGGCACGGGTTTTTCTGGTTTATCCCGCTTAGTCAGTCTTCCGGTAACGGAAATCAAAATTGAC
AAAAGCTTTGTCGATCGTTGTCTGACCGAAAAACGCATCCGAGCCTTACTTGAAGCCATTACCAGCATTGGGCAAAGCCT
CAATTTAACCGTCGTGGCAGAAGGCGTCGAAACCAAAGAACAATTTGAGATGCTACGCAAGATTCACTGTCGCGTTATTC
AGGGATATTTCTTTTCCCGCCCCCTACCCGCCGAAGAAATTCCTGGCTGGATTAGCAGCGTGTTACCGCTGAAAATCTGA

Upstream 100 bases:

>100_bases
AATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTGAACTCTGGAAAGCCAGTCTTTAGATGC
GCCAGGATGCAGAGGTAATC

Downstream 100 bases:

>100_bases
CAATTTTCTCCCGCCCGCACTCGCGGGTTTTCATTTAACGTGACACTGTCACTTAAATACTGTGATTTCAGCTACGATTC
CGGGAGATTCCTTCCTTAAC

Product: cAMP phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 799; Mature: 799

Protein sequence:

>799_residues
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGG
KARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPLHWASSSHGAEVQNAQSWS
ATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDK
AVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSK
PIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLV
YQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALH
FRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKID
KSFVDRCLTEKRIRALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWISSVLPLKI

Sequences:

>Translated_799_residues
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGG
KARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPLHWASSSHGAEVQNAQSWS
ATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDK
AVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSK
PIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLV
YQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALH
FRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKID
KSFVDRCLTEKRIRALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWISSVLPLKI
>Mature_799_residues
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGG
KARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPLHWASSSHGAEVQNAQSWS
ATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDK
AVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSK
PIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLV
YQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALH
FRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKID
KSFVDRCLTEKRIRALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWISSVLPLKI

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=799, Percent_Identity=99.2490613266583, Blast_Score=1644, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=439, Percent_Identity=34.1685649202733, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI226510982, Length=416, Percent_Identity=31.7307692307692, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=34.8739495798319, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1790496, Length=247, Percent_Identity=36.0323886639676, Blast_Score=149, Evalue=1e-36,
Organism=Escherichia coli, GI87081980, Length=261, Percent_Identity=33.7164750957854, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI1786507, Length=330, Percent_Identity=30, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=33.8775510204082, Blast_Score=127, Evalue=4e-30,
Organism=Escherichia coli, GI1788502, Length=255, Percent_Identity=29.8039215686275, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1787055, Length=275, Percent_Identity=30.5454545454545, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788849, Length=462, Percent_Identity=25.5411255411255, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082096, Length=262, Percent_Identity=27.8625954198473, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 90250; Mature: 90250

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDML
CCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHHCCCCCCEE
IPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLA
ECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
LVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYCISEASGMQ
EEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
CCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHH
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGM
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCC
PLHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAA
CEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
LALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
LGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSK
HCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC
PIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKE
CEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
RLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEE
HHHHHHHHHHHHCCCCEEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHH
IGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGID
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHHEEEECCCCCCHHHHHHHHHCCCC
GHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKID
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEC
KSFVDRCLTEKRIRALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
PLPAEEIPGWISSVLPLKI
CCCHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDML
CCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHHCCCCCCEE
IPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLA
ECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
LVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYCISEASGMQ
EEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
CCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHH
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGM
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCC
PLHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAA
CEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
LALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
LGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSK
HCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC
PIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKE
CEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
RLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEE
HHHHHHHHHHHHCCCCEEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHH
IGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGID
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHHEEEECCCCCCHHHHHHHHHCCCC
GHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKID
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEC
KSFVDRCLTEKRIRALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
PLPAEEIPGWISSVLPLKI
CCCHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]