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The map label for this gene is slrP [H]

Identifier: 218704706

GI number: 218704706

Start: 1486392

End: 1487828

Strand: Reverse

Name: slrP [H]

Synonym: ECUMN_1480

Alternate gene names: 218704706

Gene position: 1487828-1486392 (Counterclockwise)

Preceding gene: 218704708

Following gene: 218704703

Centisome position: 28.6

GC content: 48.02

Gene sequence:

>1437_bases
ATGGCCAGCATTAAAGACATCCGCCCATTCGCCCCCGGCATTCCCCCATCTTCCGTTAACCCAGGCGTACGCCCACCAGG
GCAGTTCTTCCTGCCGGTGGAACTCAAGAATGAAATTTTGCGGAACATGAAGTATATCGGCCCGCAAATATTTAGCTGGG
GTGATGACCACCGGCAGATCATTGACCTCAGTACCGCGGGCGAAGTGAAAATACATATGGTAGACAATACCATCATCACG
TTGGAGCGCCTGAATCCGAATCCGCAACATAACGAGTTCATGATGGTCGCCGAATGGTGCCAATGGGCTATGGAGCGGGA
TGGTTTGCAAGAAAACCTGCAAAAAAACCTGTACGAAATCCTGGAAGAAAACCAGCAAAACAAACAGTCAGAAATTCCCC
AAGAAGACCTGAAAGAAAGTCTGGAAGAAATCAAGGAAAATATCCTGGAAGAAAACCTGCCAGCAAGCCGGATTGAAAAC
CGGGCAGAAGCATTGCGCAGAATGAAGGAATGTCTCATTACAAGACGGAGTATGCTCAATCTTAGCAACCTTGGATTGAC
TTCACTCCCTGAAAATTTGCCTCCACATCTGATTGAATTTTACTGCAGTAAAAACGTGTTGACCGCGTTACCGAAGGTGA
TGCCAAAGTGGCTGCTGGTGCTTGACTGTACAGATAACGTCTTGATCTTGTTACCGAAGGTGCAGCCCTCAAAACTGATG
GTACTGAATTGCTATGACAACTGTATTATCTGGCTGCCTGAGCTGTCGACTAACCTGAGAGTGATTAACTGTTCTGAAAA
CTTCTTGCAATTTTTACCGCCGTCGATGCCCCAGTACCTGTATAAACTATCCTGTGCCGGCAACAATATTAACTCAATAC
CTGATGAGATGCTGGAGAACTTGACTCGCCTGAAAGTATTTGACTGTTCTAGTAACGATTTGATCTCTGCACCACGGCTG
CCGCCCAAACTAATCATATACTACTGTGGAGAAAACCAGTTTAAAACGGTACCGGTGCCGCAGCCCCGAAGCCTGAAGGT
GTTTAGCTGTAATGGTAACCCGTGGGACAAAGACAATTTACCGACGCTGCTCAAAGCCGTCGAGGGCCTGAAAAACCAGG
AGGGTCTGGAGGAGCTTTTGGACTTTTTGCACAAGGAGTGCCAGATGAAGCTGGAGAAGTTGGAGAAGCTGGAGGATCTG
GATGACCTTATGGTTCTGGAGCTCCTGGATGACCCGGTACTCCTGGAGCGCGTGAAGGTACAGGAGGACCTGGAGCTCCT
GGATCAGCAGTTGGGCCTGTTGAGTCTGGAAAGACAACAGGACTCGCAGCCTGTTAATCAACATTCTGAACATGAACCCG
AATCTGCATCAAGGGTGAAGCGTGATTTATCTGAAGTCGAGTTCGAGTCAACAATGAAACGTAAGCGTTTTATGTAG

Upstream 100 bases:

>100_bases
GTTATATCAGCGCAAATGACGTCCTGTGGCACCTTTGCGGTGACCACATTCACGTCTCCGGCGCTGCCAGAGGATATTTA
TTTCAAGGATATGTAGCATT

Downstream 100 bases:

>100_bases
AACGCCTGTGCGGTGAACTCAGGTTCCGGGGAAATAGTGTCCATCATTGTAATGGACACTATCATGGGAAATGGCTCTGT
CACATCAACAACTACAGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Secreted effector protein slrP [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMKYIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIIT
LERLNPNPQHNEFMMVAEWCQWAMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
RAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLM
VLNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISAPRL
PPKLIIYYCGENQFKTVPVPQPRSLKVFSCNGNPWDKDNLPTLLKAVEGLKNQEGLEELLDFLHKECQMKLEKLEKLEDL
DDLMVLELLDDPVLLERVKVQEDLELLDQQLGLLSLERQQDSQPVNQHSEHEPESASRVKRDLSEVEFESTMKRKRFM

Sequences:

>Translated_478_residues
MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMKYIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIIT
LERLNPNPQHNEFMMVAEWCQWAMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
RAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLM
VLNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISAPRL
PPKLIIYYCGENQFKTVPVPQPRSLKVFSCNGNPWDKDNLPTLLKAVEGLKNQEGLEELLDFLHKECQMKLEKLEKLEDL
DDLMVLELLDDPVLLERVKVQEDLELLDQQLGLLSLERQQDSQPVNQHSEHEPESASRVKRDLSEVEFESTMKRKRFM
>Mature_477_residues
ASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMKYIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITL
ERLNPNPQHNEFMMVAEWCQWAMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIENR
AEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLMV
LNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISAPRLP
PKLIIYYCGENQFKTVPVPQPRSLKVFSCNGNPWDKDNLPTLLKAVEGLKNQEGLEELLDFLHKECQMKLEKLEKLEDLD
DLMVLELLDDPVLLERVKVQEDLELLDQQLGLLSLERQQDSQPVNQHSEHEPESASRVKRDLSEVEFESTMKRKRFM

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Can ubiquitinate both ubiquitin and host TXN (Thioredoxin).

COG id: COG4886

COG function: function code S; Leucine-rich repeat (LRR) protein

Gene ontology:

Cell location: Secreted. Host cytoplasm. Note=Secreted via type III secretion systems 1 and 2 (SPI-1 and SPI-2 TTSS), and delivered into the host cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 10 LRR (leucine-rich) repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001611 [H]

Pfam domain/function: PF00560 LRR_1 [H]

EC number: NA

Molecular weight: Translated: 55191; Mature: 55059

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMKYIGPQIFSWGDDHRQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCHHHCCCCCCCEE
IDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQWAMERDGLQENLQKNLYEI
EEECCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIENRAEALRRMKECLITRRSMLN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLM
HHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCEE
VLNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLEN
EEEECCCEEEECCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
LTRLKVFDCSSNDLISAPRLPPKLIIYYCGENQFKTVPVPQPRSLKVFSCNGNPWDKDNL
HHHEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCH
PTLLKAVEGLKNQEGLEELLDFLHKECQMKLEKLEKLEDLDDLMVLELLDDPVLLERVKV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QEDLELLDQQLGLLSLERQQDSQPVNQHSEHEPESASRVKRDLSEVEFESTMKRKRFM
HHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMKYIGPQIFSWGDDHRQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCHHHCCCCCCCEE
IDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQWAMERDGLQENLQKNLYEI
EEECCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIENRAEALRRMKECLITRRSMLN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLM
HHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCEE
VLNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLEN
EEEECCCEEEECCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
LTRLKVFDCSSNDLISAPRLPPKLIIYYCGENQFKTVPVPQPRSLKVFSCNGNPWDKDNL
HHHEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCH
PTLLKAVEGLKNQEGLEELLDFLHKECQMKLEKLEKLEDLDDLMVLELLDDPVLLERVKV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QEDLELLDQQLGLLSLERQQDSQPVNQHSEHEPESASRVKRDLSEVEFESTMKRKRFM
HHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10861017 [H]