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The map label for this gene is rutF [H]

Identifier: 218704424

GI number: 218704424

Start: 1242758

End: 1243252

Strand: Reverse

Name: rutF [H]

Synonym: ECUMN_1190

Alternate gene names: 218704424

Gene position: 1243252-1242758 (Counterclockwise)

Preceding gene: 218704425

Following gene: 218704423

Centisome position: 23.9

GC content: 58.38

Gene sequence:

>495_bases
ATGAACATTGTCGATCAACAAACTTTTCGCGATGCGATGTCCTGCATGGGCGCGGCGGTCAATATTATCACCACGGACGG
TCCAGCCGGGCGCGCCGGGTTCACCGCCAGCGCCGTCTGCAGCGTGACCGATACGCCGCCCACATTACTGGTATGCCTGA
ATCGTGGGGCGTCCGTCTGGCCGGTATTCAATGAAAATCGAACGCTGTGTGTAAATACACTTAGCGCCGGACAGGAGCCG
CTTTCAAACCTTTTTGGCGGCAAAACGCCCATGGAACACCGCTTTGCCGCCGCCCGCTGGCAGACTGGTGTGACCGGATG
CCCGCAACTGGAAGAGGCACTGGTTTCGTTTGACTGCCGTATCAGCCAGGTGGTGAGCGTCGGCACCCACGACATTCTGT
TTTGCGCCATCGAAGCGATTCATCGTCACGCCACACCCTACGGGCTGGTGTGGTTCGATCGCAGTTATCACGCGCTGATG
CGCCCTGCTTGTTAA

Upstream 100 bases:

>100_bases
CTGCTGATTAATATCGGCTATGGCGATAGCAGCAAGCTTTATGCGCGCCTGCCACGTCTGTCCTTTGAAGAAGCCTGCGG
GCTGTTGTAAGGAGCAAACT

Downstream 100 bases:

>100_bases
CCTCCTTAAGGAGACAGCTGATGGCAATGTTCGGTCTTCCTCACTGGCAGTTAAAATCGACCTCTACAGAGAGCGGCGTG
GTCGCGCCGGATGAACGATT

Product: flavin:NADH oxidoreductase subunit of alternative pyrimidine degradation pathway

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEP
LSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
RPAC

Sequences:

>Translated_164_residues
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEP
LSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
RPAC
>Mature_164_residues
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEP
LSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
RPAC

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=164, Percent_Identity=98.780487804878, Blast_Score=339, Evalue=6e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 17747; Mature: 17747

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVW
CCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHEEEEECCCCCEEEEEECCCCEEE
PVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCR
EEECCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCH
ISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALMRPAC
HHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC
>Mature Secondary Structure
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVW
CCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHEEEEECCCCCEEEEEECCCCEEE
PVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCR
EEECCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCH
ISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALMRPAC
HHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA