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The map label for this gene is ybiW [H]

Identifier: 218704245

GI number: 218704245

Start: 1042140

End: 1044572

Strand: Reverse

Name: ybiW [H]

Synonym: ECUMN_1011

Alternate gene names: 218704245

Gene position: 1044572-1042140 (Counterclockwise)

Preceding gene: 218704246

Following gene: 218704244

Centisome position: 20.08

GC content: 55.16

Gene sequence:

>2433_bases
ATGACCACACTGAAACTGGACACGCTCAGCGATCGCATTAAAGCGCACAAAAATGCGCTGGTGCATATTGTGAAACCGCC
GGTCTGTACCGAGCGCGCGCAGCACTATACCGAGATGTATCAACAACATCTCGATAAGCCGATCCCGGTACGTCGCGCGC
TGGCACTGGCGCATCACCTGGCGAATCGCACCATCTGGATCAAGCACGATGAGTTGATCATTGGCAACCAGGCAAGCGAA
GTTCGCGCCGCGCCGATCTTCCCGGAATATACTGTCTCATGGATCGAAAAAGAGATCGATGATCTGGCAGATCGTCCCGG
TGCTGGCTTTGCGGTGAGCGAAGAGAACAAACGCGTTCTGCATGAAGTGTGCCCGTGGTGGCGCGGTCAGACCGTACAGG
ATCGCTGCTACGGCATGTTTACCGATGAGCAAAAAGGTCTGCTGGCGACCGGCATCATTAAAGCGGAAGGCAATATGACC
TCCGGCGATGCGCACCTGGCGGTCAATTTCCCGCTGCTGCTGGAAAAAGGGCTTGATGGTCTGCGCGAGAAAGTAGCGGA
ACGTCGCTCGCGCATCAACCTGACGGTGCTGGAAGATTTACATGGCGAGCAATTCCTGAAAGCGATTGATATCGTGCTGG
TAGCGGTCAGTGAACACATTGAACGTTTCGCTGCCCTGGCGCGTGAAATGGCCGCGACCGAAACTCGCGAAAGCCGTCGC
GATGAACTGCTGGCGATGGCAGAAAACTGCGATCTTATCGCCCACCAACCGCCGCAGACTTTCTGGCAGGCGCTGCAACT
GTGTTACTTCATCCAGTTGATTTTGCAGATCGAATCTAACGGTCACTCAGTATCTTTTGGTCGTATGGACCAGTATCTGT
ACCCGTATTATCGTCGCGACGTTGAACTCAACCAGACGCTGGATCGTGAACACGCCATCGAGATGCTGCATAGCTGCTGG
CTGAAATTGCTGGAAGTGAACAAGATCCGCTCCGGCTCGCACTCAAAAGCCTCTGCGGGAAGTCCGCTGTATCAGAACGT
CACTATTGGCGGGCAAAATCTGGTTGATGGTCAACCAATGGACGCGGTGAATCCACTCTCTTACGCGATCCTTGAATCCT
GCGGTCGCCTGCGTTCCACTCAGCCTAACCTCAGCGTGCGTTACCACGCGGGAATGAGCAACGATTTCCTCGACGCCTGC
GTACAGGTGATCCGTTGCGGCTTCGGGATGCCGGCGTTCAACAACGACGAAATCGTGATCCCGGAATTTATTAAACTCGG
TATTGAACCGCAGGACGCTTACGACTATGCAGCGATTGGTTGTATCGAAACCGCCGTCGGTGGCAAATGGGGCTATCGCT
GTACCGGCATGAGCTTTATCAACTTCGCCCGCGTGATGCTGGCGGCGCTGGAAGGCGGTCGTGATGCCACCAGCGGCAAA
GTGTTCCTGCCACAAGAAAAAGCGTTGTCGGCGGGTAACTTCAACAACTTCGATGAAGTAATGGACGCATGGGATACGCA
AATCCGTTACTACACGCGTAAATCAATCGAAATCGAATATGTCGTTGACACCATGCTGGAGGAGAACGTGCACGATATTC
TCTGCTCGGCGCTGGTGGATGACTGTATTGAGCGAGCAAAAAGTATCAAGCAAGGCGGCGCGAAATATGACTGGGTTTCT
GGCCTGCAGGTCGGCATTGCCAACCTCGGCAACAGCCTGGCGGCAGTGAAGAAACTGGTGTTTGAACAGGGCGCGATTGG
TCAGCAACAACTGGCTGCCGCACTGGCGGATGACTTCGACGGCCTGACTCACGAGCAACTGCGTCAGCGGCTGATTAACG
GCGCGCCGAAGTACGGCAACGACGACGATACTGTCGATACGCTGCTGGCTCGCGCTTATCAGACCTATATCGACGAACTG
AAACAGTACCATAACCCGCGCTACGGTCGTGGTCCGGTTGGCGGCAATTATTACGCGGGTACGTCGTCTATCTCCGCTAA
CGTACCGTTTGGCGCGCAAACTATGGCAACGCCGGACGGACGTAAAGCGCATACCCCGCTGGCAGAAGGCGCAAGCCCGG
CCTCCGGTACTGACCATCTTGGCCCTACCGCGGTCATTGGCTCGGTGGGTAAACTGCCTACGGCCGCGATTCTCGGCGGC
GTGCTGCTCAACCAGAAACTGAATCCAGCAACGCTGGAGAACGAATCTGACAAGCAGAAACTGATGATCCTGCTGCGCAC
CTTCTTCGAGGTGCATAAAGGCTGGCATATTCAGTACAACATCGTTTCCCGCGAAACGCTGCTGGAAGCGAAAAAACATC
CGGATCAGTACCGCGATCTGGTAGTGCGTGTCGCGGGCTATTCCGCCTTCTTCACCGCGCTCTCTCCAGACGCTCAGGAC
GATATCATCGCCCGTACTGAACATATGCTGTAA

Upstream 100 bases:

>100_bases
ACGCCCCGGAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCCAGCAGTATGCCTGCCAGAAAGGGTTAACCGCG
ACCTTACGAGGATAACAACC

Downstream 100 bases:

>100_bases
TCTCCTCAGCCCGGCGGCGGCGTCGCCGGGCTAAATCATTCACATCATCAATTTCAGCCTCGCTTTCATTCGAAATATAA
TTTGTGCTCTGCGTCACATT

Product: putative glycyl radical cofactor protein

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
DELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML

Sequences:

>Translated_810_residues
MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
DELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML
>Mature_809_residues
TTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEV
RAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTS
GDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRD
ELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWL
KLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACV
QVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGKV
FLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSG
LQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELK
QYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGV
LLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDD
IIARTEHML

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=99.6296296296296, Blast_Score=1683, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=805, Percent_Identity=37.7639751552795, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994926, Length=574, Percent_Identity=28.2229965156794, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1787131, Length=563, Percent_Identity=27.3534635879218, Blast_Score=179, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90159; Mature: 90027

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHL
CCEEECHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
HEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHH
LREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHL
CEEECHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
HEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHH
LREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]