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The map label for this gene is ybiY [H]

Identifier: 218704246

GI number: 218704246

Start: 1044578

End: 1045504

Strand: Reverse

Name: ybiY [H]

Synonym: ECUMN_1012

Alternate gene names: 218704246

Gene position: 1045504-1044578 (Counterclockwise)

Preceding gene: 218704248

Following gene: 218704245

Centisome position: 20.1

GC content: 53.61

Gene sequence:

>927_bases
ATGTTTGAACGAAACAGAGAGGCAACTATGATTTTCAATATTCAGCGCTACTCGACCCATGATGGCCCCGGTATCCGCAC
GGTCGTATTTCTTAAAGGCTGTTCGCTGGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGCGCCCGCACGCAAGATCTGC
TGTATGACCCACGACTCTGTCTGGAAGGCTGTGAGCTGTGCGCTAAGGCCGCGCCGGAAGTGATTGAGCGCGCGTTGAAT
GGTTTGCTTATTCATCGAGAAAAGTTAACCCCGGAGCATTTTACGGCGCTAACCGACTGCTGCCCGACACAGGCATTAAC
CGTGTGTGGTGAAGTGAAAAGCGTTGAGGAGATCATGGCGACCGTTTTGCGGGATAAACCGTTTTACGATCGCAGCGGCG
GCGGCTTAACGCTTTCGGGCGGTGAACCGTTTATGCAGCCGGAAATGGCGACGGCGCTACTGCAAGCCAGCCACGAGGCC
GGCATTCATACTGCGGTAGAAACCTGTCTGCATGTGCCGTGGAAATATATCGCCCCTTCTCTGCCCTATATCGATCTGTT
TCTTGCCGATTTAAAACACGTTGCCGACGCGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGAGTGCTGGATAACC
TGAAAAAACTCGCCGCAGCGGGCAAAAAAATCATTATCCGCGTGCCGCTGATTCAGGGCTTTAATGCCGACGAAACCTCT
GTAAAAGCCATTACCGATTTTGCCGCCGACGAGCTGCACGTTAGCGAAATTCATTTCCTGCCCTACCACACGCTGGGCAT
CAACAAATATCACTTACTTAATCTGCCCTATGACGCCCCGGAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCC
AGCAGTATGCCTGCCAGAAAGGGTTAACCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
CATCCTCAAAATGTACTTTTTGTCTGAATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACGCACATCAATCTAAC
TTTCATTCGAAAGTATTTTA

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACGCTCAGCGATCGCATTAAAGCGCACAAAAATGCGCTGGTGCATATTGTGAAA
CCGCCGGTCTGTACCGAGCG

Product: putative AdoMet-dependent glycyl radical activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDPRLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHFTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDPRLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHFTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
>Mature_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDPRLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHFTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=98.3277591973244, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1790389, Length=307, Percent_Identity=35.1791530944625, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=32.7485380116959, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790839, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 34204; Mature: 34204

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDPRLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHCCHHHH
LEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCCHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHHCCEEHHEEEECCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC
>Mature Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDPRLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHCCHHHH
LEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCCHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHHCCEEHHEEEECCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]