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The map label for this gene is leuS [H]

Identifier: 218703976

GI number: 218703976

Start: 785705

End: 788287

Strand: Reverse

Name: leuS [H]

Synonym: ECUMN_0736

Alternate gene names: 218703976

Gene position: 788287-785705 (Counterclockwise)

Preceding gene: 218703978

Following gene: 218703975

Centisome position: 15.15

GC content: 53.31

Gene sequence:

>2583_bases
ATGCAAGAGCAATACCGCCCGGAAGAGATAGAATCCAAAGTACAGCTTCATTGGGATGAGAAGCGCACATTTGAAGTAAC
CGAAGACGAGAGCAAAGAGAAGTATTACTGCCTGTCTATGCTTCCCTATCCTTCTGGTCGACTACACATGGGCCACGTAC
GTAACTACACCATCGGTGACGTGATCGCCCGCTACCAGCGTATGCTGGGCAAAAACGTCCTGCAGCCGATCGGCTGGGAC
GCGTTTGGTCTGCCTGCGGAAGGCGCGGCGGTGAAAAACAACACCGCACCGGCACCGTGGACGTACGACAACATCGCGTA
CATGAAAAACCAGCTCAAAATGCTGGGCTTTGGTTATGACTGGAGCCGCGAGCTGGCAACCTGTACGCCGGAATACTACC
GTTGGGAACAGAAATTCTTCACCGAGCTGTATAAAAAAGACCTGGTATATAAGAAGACTTCTGCGGTCAACTGGTGCCCG
AACGACCAGACCGTACTGGCGAACGAACAAGTTATCGACGGCTGCTGCTGGCGCTGCGATACCAAAGTTGAGCGTAAAGA
GATCCCGCAATGGTTTATCAAAATCACCGCTTACGCTGACGAGCTGCTCAACGATCTGGATAAACTGGATCACTGGCCAG
ACACCGTTAAAACCATGCAGCGTAACTGGATTGGTCGTTCCGAAGGCGTAGAAATCACTTTCAACGTTAACGACTATGAC
AACACGCTGACCGTTTACACTACCCGCCCGGACACCTTTATGGGTTGTACCTACCTGGCGGTAGCGGCAGGTCATCCGCT
GGCACAGAAAGCGGCGGAAAATAATCCTGAACTGGCGGCCTTTATTGACGAATGCCGTAACACCAAAGTTGCCGAAGCTG
AAATGGCAACGATGGAGAAAAAAGGCGTCGATACTGGCTTTAAAGCGGTTCACCCATTAACGGGCGAAGAAATTCCCGTT
TGGGCAGCAAACTTCGTATTGATGGAGTACGGCACGGGCGCAGTTATGGCGGTTCCGGGTCACGACCAGCGCGACTACGA
GTTTGCCTCTAAATACGGCCTGAACATCAAACCGGTTATCCTGGCAGCTGACGGCTCTGAGCCAGATCTTTCTCAGCAAG
CCCTGACTGAAAAAGGCGTTCTGTTCAACTCTGGCGAGTTCAACGGTCTTGACCATGAAGCGGCCTTCAACGCTATCGCC
GATAAACTGACTGCGATGGGCGTTGGCGAGCGTAAAGTGAACTACCGCCTGCGCGACTGGGGTGTTTCCCGTCAACGTTA
CTGGGGCGCGCCGATTCCGATGGTGACGCTGGAAGACGGTACCGTAATGCCGACCCCGGACGACCAGCTGCCGGTGATCC
TGCCGGAAGATGTGGTGATGGACGGCATTACCAGCCCGATTAAAGCAGATCCGGAGTGGGCAAAAACTACCGTTAACGGT
ATGCCAGCGCTGCGTGAAACCGACACTTTCGACACCTTTATGGAGTCCTCCTGGTACTATGCGCGCTACACTTGCCCGGA
GTACAAAGAAGGTATGCTGGATTCCAAAGCGGCTAACTACTGGCTGCCGGTGGATATCTACATTGGTGGTATCGAACACG
CCATTATGCACCTGCTCTACTTCCGCTTCTTCCACAAACTGATGCGTGATGCAGGCATGGTGAACTCTGACGAACCAGCG
AAACAGTTGCTGTGTCAGGGTATGGTGCTGGCAGATGCCTTCTACTATGTTGGCGAAAACGGCGAACGTAACTGGGTTTC
CCCGGTTGATGCTATCGTTGAACGTGACGAGAAAGGCCGTATCGTGAAAGCGAAAGATGCGGCAGGCCATGAACTGGTTT
ATACCGGCATGAGCAAAATGTCCAAGTCTAAGAACAACGGTATCGACCCGCAGGTGATGGTTGAACGTTACGGCGCGGAC
ACCGTTCGTCTGTTTATGATGTTTGCTTCTCCGGCTGATATGACTCTCGAATGGCAGGAATCCGGCGTGGAAGGGGCTAA
CCGCTTCCTGAAACGTGTCTGGAAACTGGTTTACGAGCACACAGCGAAAGGTGATGTTGCGGCACTGAACGTTGATGCGC
TGACTGAAGATCAGAAAGCGCTGCGTCGCGATGTGCATAAAACGATCGCTAAAGTGACCGATGATATCGGCCGTCGTCAG
ACCTTCAACACCGCAATTGCGGCGATTATGGAGCTGATGAACAAACTGGCGAAAGCACCGACCGATGGCGAGCAGGACCG
CGCCCTGATGCAGGAAGCGCTGCTGGCGGTAGTCCGTATGCTTAACCCGTTCACCCCGCACATCTGCTTCACGCTGTGGC
AGGAACTGAAAGGCGAAGGCGATATCGACAACGCACCGTGGCCGGTTGCTGACGAAAAAGCGATGGTGGAAGACTCCACG
CTGGTCGTGGTGCAGGTTAACGGTAAAGTCCGTGCAAAAATCACCGTTCCGGTGGACGCAACGGAAGAACAGGTTCGCGA
ACGTGCTGGCCAGGAACATCTGGTAGCAAAATATCTTGATGGCGTTACCGTGCGTAAAGTGATTTACGTACCCGGTAAAC
TCCTCAATCTGGTCGTTGGCTAA

Upstream 100 bases:

>100_bases
GGCTGTTTTGACGCGGACGTTTGGGCTATGCTATGCGGATCTGAAAAACCACCTCAACGCTACATTTGTAGCCGTATTGA
AAACAGGACCACTGGCTGCC

Downstream 100 bases:

>100_bases
GCGCGGGAGGAAGCGTGCGATATCTGGCAACATTGTTGTTATCTCTGGCGGTGTTAATCACCGCCGGGTGTGGCTGGCAT
CTGCGTGATACCACGCAGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Sequences:

>Translated_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG
>Mature_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=865, Percent_Identity=36.1849710982659, Blast_Score=507, Evalue=1e-143,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=99.5348837209302, Blast_Score=1787, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=398, Percent_Identity=26.8844221105528, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71997510, Length=881, Percent_Identity=29.738933030647, Blast_Score=351, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71997517, Length=873, Percent_Identity=29.7823596792669, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI212645227, Length=377, Percent_Identity=27.5862068965517, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71980946, Length=151, Percent_Identity=28.476821192053, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554638, Length=86, Percent_Identity=38.3720930232558, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=809, Percent_Identity=37.3300370828183, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321531, Length=387, Percent_Identity=26.3565891472868, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325217, Length=357, Percent_Identity=23.249299719888, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355409, Length=882, Percent_Identity=32.9931972789116, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI281366294, Length=471, Percent_Identity=22.5053078556263, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 97294; Mature: 97294

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHEECCCC
WSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCEEEE
LAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDW
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEEEECCCCCCEEEEHH
SGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC
>Mature Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHEECCCC
WSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCEEEE
LAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDW
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEEEECCCCCCEEEEHH
SGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA