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The map label for this gene is folK [H]

Identifier: 218703398

GI number: 218703398

Start: 161238

End: 161717

Strand: Reverse

Name: folK [H]

Synonym: ECUMN_0139

Alternate gene names: 218703398

Gene position: 161717-161238 (Counterclockwise)

Preceding gene: 218703399

Following gene: 218703397

Centisome position: 3.11

GC content: 47.71

Gene sequence:

>480_bases
ATGACAGTGGCGTATATTGCCATAGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCTGCCCTGAAAGCATTAGG
CGATATCCCTGAAAGCCACATTCTTGCCGTTTCTTCGTTTTACCGCACTCCACCGCTGGGGCCGCAAGATCAACCCGATT
ACTTAAACGCAGCCGTGGCGCTGGAAACCTCTCTTGCACCTGAAGAGTTACTCAACCACACACAACGTATTGAGTTACAA
CAAGGTCGCGTCCGTAAAGCGGAACGCTGGGGGCCGCGCACACTGGATCTCGACATCATGCTATTTGGTAATGAAGTAAT
AAATACCGAACGCCTGACCGTTCCGCACTACGATATGAAGAATCGTGGATTTATGCTGTGGCCGCTGTTTGAAATTGCGC
CGGAGCTGGTGTTTCCTGATGGAACAGTATTAGGAAATTTATTAAAAATAAATAAATTTCATGAATTAAACAAATGGTGA

Upstream 100 bases:

>100_bases
CAGATCAAAAAGGGATGCTCAACGAGCTGGATGAAGAGCCGTCACCGCGTCGCCGTTCTCGTCGTCCACGCAAACGCGCA
CCGCGTCGTGAGGGTACCGC

Downstream 100 bases:

>100_bases
TTTAATGATTTCATGAATATAAATAAATTCCGAAAAATAGCCTTACCCCATTGCAATTAATATATTCCCTAAGCTACAAC
CATAATTAATATTATATACC

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW

Sequences:

>Translated_159_residues
MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW
>Mature_158_residues
TVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=92.4528301886792, Blast_Score=302, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6324073, Length=142, Percent_Identity=34.5070422535211, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17917; Mature: 17786

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTPPLGPQDQPDYLNAAVA
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEEE
LETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
EECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCEEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW
CCCEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTPPLGPQDQPDYLNAAVA
EEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEEE
LETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
EECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCEEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW
CCCEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]