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The map label for this gene is pcnB [H]

Identifier: 218703399

GI number: 218703399

Start: 161714

End: 163132

Strand: Reverse

Name: pcnB [H]

Synonym: ECUMN_0140

Alternate gene names: 218703399

Gene position: 163132-161714 (Counterclockwise)

Preceding gene: 218703400

Following gene: 218703398

Centisome position: 3.14

GC content: 55.39

Gene sequence:

>1419_bases
TTGACACTACCGAGGTGTACTATTTTTACCCGAGTCGCTAATTTTTGCCGCAAGGTGCTAAGCCGCGAGGAAAGCGAGGC
TGAACAGGCAGTCGCCCGTCCACAGGTGACGGTGATCCCGCGTGAGCAGCATGCTATTTCCCGCAAAGATATCAGTGAAA
ATGCCCTGAAGGTAATGTACAGGCTCAATAAAGCGGGATACGAAGCCTGGCTGGTTGGCGGCGGCGTGCGCGACCTGTTA
CTTGGCAAAAAGCCGAAAGATTTTGACGTAACCACTAACGCCACGCCTGAACAGGTACGTAAACTGTTCCGTAACTGCCG
CCTGGTGGGTCGCCGTTTCCGTCTGGCTCATGTGATGTTTGGCCCGGAGATTATCGAAGTTGCGACCTTCCGTGGACATC
ACGAAGGTAACGTCAGCGACCGCACGACCTCCCAACGCGGGCAAAACGGTATGTTGCTGCGCGACAACATTTTCGGCTCC
ATCGAAGAAGACGCCCAGCGCCGCGATTTCACTATCAACAGCCTGTATTACAGCGTAGCGGATTTTACCGTCCGTGATTA
CGTTGGCGGCATGAAGGATCTGAAAGACGGCGTTATCCGTCTGATTGGTAACCCGGAAACGCGCTACCGTGAAGATCCGG
TACGGATGCTGCGCGCGGTACGTTTTGCCGCCAAATTGGGTATGCGTATCAGTCCTGAAACCGCAGAACCGATCCCTCGC
CTCGCTACCCTGCTGAACGATATTCCACCGGCACGTCTGTTTGAAGAATCTTTGAAACTGCTGCAAGCGGGCTACGGTTA
CGAAACCTATAATCTGCTGTGTGAATATCACCTGTTCCAGCCGCTGTTCCCGACCATTACCCGCTACTTCACGGAAAATG
GCGACAGCCCGATGGAGCGGATCATTGAACAGGTGCTGAAGAATACCGATACGCGTATCCATAACGATATGCGCGTGAAC
CCGGCGTTCCTGTTTGCCGCCATGTTCTGGTACCCACTGCTGGAGACGGCACAGAAGATCGCCCAGGAAAGCGGCCTGAC
CTATCACGACGCTTTCGCGCTGGCGATGAACGACGTGCTGGACGAAGCCTGCCGTTCTCTGGCGATCCCGAAACGTCTGA
CGACGTTAACCCGCGATATCTGGCAGTTGCAGTTGCGTATGTCCCGACGTCAGGGTAAACGCGCCTGGAAACTGCTGGAG
CATCCTAAGTTCCGTGCTGCTTATGACCTGTTGGCCTTGCGAGCTGAAGTTGAACGTAACGCTGAACTGCAGCGTCTTGT
GAAATGGTGGGGGGAGTTCCAGGTTTCCGCGCCACCAGATCAAAAAGGGATGCTCAACGAGCTGGATGAAGAGCCGTCAC
CGCGTCGCCGTTCTCGTCGTCCACGCAAACGCGCACCGCGTCGTGAGGGTACCGCATGA

Upstream 100 bases:

>100_bases
GGCTCACCGCAGTGCCTGAGTCGGCAATTGTAAATTCAACATTCTCAAATGCGTCATGCTGAGCTATGATTAGCCGCTAT
TTTTTTGTCCTGAATGATGT

Downstream 100 bases:

>100_bases
CAGTGGCGTATATTGCCATAGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCTGCCCTGAAAGCATTAGGCGAT
ATCCCTGAAAGCCACATTCT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLL
LGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVN
PAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLE
HPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRSRRPRKRAPRREGTA

Sequences:

>Translated_472_residues
MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLL
LGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVN
PAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLE
HPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRSRRPRKRAPRREGTA
>Mature_471_residues
TLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLL
GKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSI
EEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPRL
ATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNP
AFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEH
PKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRSRRPRKRAPRREGTA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=274, Percent_Identity=31.021897810219, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081691, Length=465, Percent_Identity=99.3548387096774, Blast_Score=954, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=323, Percent_Identity=27.8637770897833, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=261, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=293, Percent_Identity=26.2798634812287, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281360112, Length=237, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357337, Length=237, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54629; Mature: 54497

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMY
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLFPTITRYFTENGDSPMER
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVL
HHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
AELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRSRRPRKRAPRREGTA
HHHHHHHHHHHCEECCCCCCCCCHHHHHCCCCCCHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
TLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLFPTITRYFTENGDSPMER
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVL
HHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
AELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRSRRPRKRAPRREGTA
HHHHHHHHHHHCEECCCCCCCCCHHHHHCCCCCCHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]