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The map label for this gene is prfC

Identifier: 218703121

GI number: 218703121

Start: 5099846

End: 5101459

Strand: Direct

Name: prfC

Synonym: ECIAI39_4903

Alternate gene names: 218703121

Gene position: 5099846-5101459 (Clockwise)

Preceding gene: 218703117

Following gene: 218703122

Centisome position: 99.37

GC content: 53.9

Gene sequence:

>1614_bases
TTGTACATAATCAACGAGACAACAATTATGACAAGCCTTTTGGCCGCAGAGGTAGCGAAAAGAAGAACCTTTGCAATTAT
TTCTCACCCGGACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAG
TAAAAGGCCGTGGCTCTAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATCACTACG
TCTGTGATGCAGTTTCCGTATCACGACTGCCTGGTTAACTTGCTCGATACCCCGGGGCACGAAGACTTCTCGGAAGATAC
CTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTAGAAGATCGTACCCGTAAGC
TGATGGAAGTTACCCGTCTGCGCGATACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATG
GAACTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGCGCACCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTT
TAAAGGCGTTTACCACCTTTATAAAGATGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCA
TTGTTAAAGGGCTGAATAACCCGGATCTCGACGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAG
CTGGTGAAAGGCGCGTCTAATGAGTTCGACAAAGAGCTGTTCCTTGCGGGCGAAATCACCCCGGTATTCTTTGGTACTGC
GCTGGGTAACTTCGGCGTCGATCATATGCTGGATGGCCTGGTGGAGTGGGCGCCTGCGCCGATGCCGCGTCAGACCGATA
CCCGTACCGTAGAAGCGAGCGAAGATAAATTTACCGGCTTCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGC
GACCGCGTGGCGTTTATGCGCGTGGTATCCGGTAAATATGAAAAAGGCATGAAGCTGCGCCAGGTGCGCACCGCGAAAGA
TGTGGTGATCTCCGACGCGCTGACCTTTATGGCAGGCGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCG
GCCTGCACAATCACGGCACCATTCAGATCGGCGATACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAAC
TTCGCGCCAGAACTGTTCCGTCGTATCCGCCTGAAAGATCCGCTGAAGCAAAAACAGCTGCTCAAAGGGCTGGTACAGCT
TTCCGAAGAGGGGGCGGTGCAGGTGTTCCGTCCGATCTCCAACAACGATCTGATCGTTGGCGCGGTTGGTGTGCTGCAGT
TTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACCGCCCGC
TGGGTAGAATGTGCGGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGA
TAACCTCGCTTACATCGCTACCAGCATGGTCAATCTGCGCCTGGCGCAGGAACGTTATCCGGACGTTCAGTTCCACCAGA
CCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
TGAGCTTTGGCTACAAATACACCTACTGATCATGTTCTGCTATGCTCTGATACCTCCAAAATGTACAATCAGCGGCATGA
ATTTTTCTTTGTACATATGA

Downstream 100 bases:

>100_bases
TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA
CATTCACTTTTGCTTATGTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MYIINETTIMTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITT
SVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPM
ELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELE
LVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHR
DRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATAR
WVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Sequences:

>Translated_537_residues
MYIINETTIMTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITT
SVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPM
ELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELE
LVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHR
DRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATAR
WVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
>Mature_537_residues
MYIINETTIMTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITT
SVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPM
ELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELE
LVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHR
DRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATAR
WVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=28.1124497991968, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI19923640, Length=526, Percent_Identity=26.2357414448669, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI25306283, Length=409, Percent_Identity=27.8728606356968, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=498, Percent_Identity=25.9036144578313, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=28.4023668639053, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=28.9156626506024, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=100, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=26.1663286004057, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=30.6976744186047, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=516, Percent_Identity=26.5503875968992, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=31.7073170731707, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=27.0676691729323, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=27.0676691729323, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=28.4232365145228, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=433, Percent_Identity=26.0969976905312, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=179, Percent_Identity=24.5810055865922, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24582462, Length=494, Percent_Identity=26.1133603238866, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.3761996161228, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60408; Mature: 60408

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIINETTIMTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSN
CEEECHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMV
CCHHHHHHHHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWP
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
IGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELE
CCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEAS
HHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECC
EDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
RSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQL
HHHHHHCCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHH
LKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATAR
HHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
WVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MYIINETTIMTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSN
CEEECHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMV
CCHHHHHHHHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWP
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
IGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELE
CCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEAS
HHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECC
EDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
RSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQL
HHHHHHCCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHH
LKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATAR
HHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
WVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA