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The map label for this gene is melA

Identifier: 218702783

GI number: 218702783

Start: 4749645

End: 4751000

Strand: Direct

Name: melA

Synonym: ECIAI39_4543

Alternate gene names: 218702783

Gene position: 4749645-4751000 (Clockwise)

Preceding gene: 218702775

Following gene: 218702784

Centisome position: 92.55

GC content: 51.92

Gene sequence:

>1356_bases
ATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCA
TCGTGAGGCGCTGAAAACGGCGCATATTGCCCTGATGGATATCGATCCCACCCGCCTGGAAGAGTCGCACATTGTGGTGC
GCAAGTTGATGGATTCAGCAGGAGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTACAGGATGCTGAT
TTTGTGGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCT
GGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTT
GCGAGGACATGACGGAAGTCTGCCCTGATGCCACCATGCTCAACTACGTTAACCCAATGGCGATGAATACCTGGGCGATG
TATGCCCGCTATCCGCATATCAAGCAGGTAGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGCTGGCGCGTGACCT
CAATATCGATCCGGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGTAAAA
CCGCAGACGGTAGCTACGTGAATCTCTATCCTGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATT
CATGGCAATACTCGCTGCCAGAATATTGTGCGCTATGAAATGTTCAAAAAGCTGGGCTACTTCGTCACAGAATCGTCAGA
ACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATG
AGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAGAACGCCTCCCGGATTGAT
ATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGT
CCGTAACGATGGATTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTC
AGCCGACCAAAGCCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACA
GAAGCTATTCTTACGGAAAATCGCGACCGTGTTTACCACGCCGCGATGATGGACCCGCATACTGCCGCCGTGCTGGGCAT
TGACGAAATATATGCTCTTGTTGACGACTTGATTGCTGCCCACGGCGACTGGCTGCCTGGCTGGTTGCACCGTTAA

Upstream 100 bases:

>100_bases
GCAGGATCTGAGTTTATGGGAATGCTCAACCGGGAAACCGGAGGTTTTCTGCGATATCGCCTGCCATGATGAAGTCATTC
AAGTAAGCCAGGAGATCTGC

Downstream 100 bases:

>100_bases
AACGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTT
GGTAACAGCGACCCGATACC

Product: alpha-galactosidase, NAD(P)-binding

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Sequences:

>Translated_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
>Mature_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=99.3348115299335, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=25.8695652173913, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50615; Mature: 50615

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKAGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKAGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]