Click here to switch to the map view.

The map label for this gene is melB

Identifier: 218702784

GI number: 218702784

Start: 4751115

End: 4752524

Strand: Direct

Name: melB

Synonym: ECIAI39_4544

Alternate gene names: 218702784

Gene position: 4751115-4752524 (Clockwise)

Preceding gene: 218702783

Following gene: 218702790

Centisome position: 92.58

GC content: 46.1

Gene sequence:

>1410_bases
ATGACTACAAAACTCAGTTATGGATTTGGAGCGTTCGGGAAGGATTTTGCGATCGGCATTGTGTATATGTACCTCATGTA
TTACTACACCGATGTCGTCGGGCTGTCTGTGGGTTTGGTCGGTACTTTGTTTCTGGTGGCGAGGATCTGGGATGCTATTA
ACGATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACG
TTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCTTTGTTTGCGT
GACCTACATCCTCTGGGGCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCATAACGCTCGATA
AACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCGGGCTTTGTTACGGCAGGTGTGACGCTA
CCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTAT
TGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAGCCGTCTGCTGAAGGAAGCCATC
TGACACTTAAAGCCATCGTTGCGCTTATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAAT
GTAGCCAGCAATATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTA
TCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTT
TATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTC
GTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGA
TACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGG
GCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACG
CAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCCACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCG
TTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGC
CTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGA

Upstream 100 bases:

>100_bases
CTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTTGGTAACAGCGACCC
GATACCCTATGAGCATTTCA

Downstream 100 bases:

>100_bases
CGTGGACAATAGCTAACGAAAATGGCCTGATGTGTTATGTTTATCAGGCCACTTCTTAATACAATGCTTTGGATTTGCAA
ATTTTGTAGACCGGATAAGG

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGT
LANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMALAYN
VASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNV
VLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQST
QALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA

Sequences:

>Translated_469_residues
MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGT
LANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMALAYN
VASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNV
VLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQST
QALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
>Mature_468_residues
TTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTL
ANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLP
FVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMALAYNV
ASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVV
LIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQ
ALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA

Specific function: Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=469, Percent_Identity=99.7867803837953, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=464, Percent_Identity=28.2327586206897, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1786466, Length=464, Percent_Identity=28.0172413793103, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI48994989, Length=444, Percent_Identity=26.3513513513513, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI145693206, Length=450, Percent_Identity=26.4444444444444, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787902, Length=460, Percent_Identity=27.8260869565217, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52207; Mature: 52075

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH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HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6323466; 7610040; 9278503; 1730719; 8672452; 1872836 [H]