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The map label for this gene is nrfE

Identifier: 218702740

GI number: 218702740

Start: 4700445

End: 4702103

Strand: Direct

Name: nrfE

Synonym: ECIAI39_4496

Alternate gene names: 218702740

Gene position: 4700445-4702103 (Clockwise)

Preceding gene: 218702739

Following gene: 218702741

Centisome position: 91.59

GC content: 57.63

Gene sequence:

>1659_bases
GTGTTGACCCCGTTGACGGCCTTTGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCT
GGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTACTGTTTTCTCATCAGCGATTTCTCGGTCATTTATG
TCGCCCAACATAGCTACAGCCTGCTGTCGTGGGAACTCAAACTGGCGGCGGTGTGGGGCGGTCATGAAGGTTCGCTGCTG
CTTTGGGTGCTGTTGCTTTCCGCCTGGAGCGCGCTGTTTGCCTGGCATTATCGGCAGCAAACCGATCCGCTATTTCCGCT
GACGCTAGCCGTTTTATCTCTCATGCTCGCCGCACTGCTACTGTTTGTGGTGCTGTGGTCCGATCCCTTCGTGCGGATAT
TTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTTCATCCGCCGCTGCTTTAT
CTCGGCTATGGCGGTTTGATGGTGGCAGCGAGCGTGGCGCTGGGGAGTTTACTGCGCGGCGAGTTTGATGGTGCCAGCGC
CCGAATTTGCTGGCGCTGGGCACTACCTGGCTGGAGCGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTATT
GCGAACTGGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCG
CTGCTGCACAGTTTGTCCCTGACACGCCAGCGGGGGATTTTCCGCCACTGGTCGCTGTTACTGGCGATAGTCACTCTGAT
GCTGTCGCTGCTGGGTACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATGCGTTCGCGCTGGATAACGTCCGCG
CCGTGCCGTTGTTCACCCTGCTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTATGGCTGGCGAGCGCGGGACGGTGGC
CCGGCGGTGCATTTTTCGGGGTTATCGCGGGAAATGTTAATCCTTGCTACGCTGTTGCTGTTTTGCGCAGTGCTACTGAT
CGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGCCTGCTGGGCTGGGGACGCCTCTCCGTTGGCGCGCCGTATTTTA
ACCGCGCGACGTTACCGTTTGGTCTGTTGATGCTGGTGGTGATTGTGCTGGCGACGTTTGTCTCTGGCAAACGCGTGCAG
CTTCCGGCGCTGCTGGCGCATGCGGGCGTGCTGTTATTTGCCGCAGGGATCGTGGTCTCCAGCGTCAGCCGTCAGGAGAT
CAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGCGTCTCGATCTGCAAGCCA
GAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGTATTGGTGAACTGACGCCGGAGCGG
CGTTTTTATGAAGCACGCCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCAT
GGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGAT
TGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAGGAAGCGCGATGAATAA

Upstream 100 bases:

>100_bases
GGGCAGTTAACGGTGGCGTAAGCCAGAACAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGT
TGTTAAGTCTCGGGGTCAAC

Downstream 100 bases:

>100_bases
AGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGTGCTCAGGTCGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGCAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLLALISLASLALYGWRARDGG
PAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Sequences:

>Translated_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLLALISLASLALYGWRARDGG
PAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE
>Mature_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLLALISLASLALYGWRARDGG
PAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=552, Percent_Identity=98.1884057971015, Blast_Score=1069, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=584, Percent_Identity=38.1849315068493, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 61436; Mature: 61436

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALGSLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHC
EFDGASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCHHHHHHH
LALISLASLALYGWRARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALLAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHCCCHHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHEEEEEEHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC
>Mature Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALGSLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHC
EFDGASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCHHHHHHH
LALISLASLALYGWRARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALLAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHCCCHHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHEEEEEEHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]