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The map label for this gene is nrfF

Identifier: 218702741

GI number: 218702741

Start: 4702096

End: 4702479

Strand: Direct

Name: nrfF

Synonym: ECIAI39_4497

Alternate gene names: 218702741

Gene position: 4702096-4702479 (Clockwise)

Preceding gene: 218702740

Following gene: 218702742

Centisome position: 91.62

GC content: 52.08

Gene sequence:

>384_bases
ATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGTGCTCAGGTCGTAGACACCTGGCAATTCGC
CAATCCGCAACAACAGCAACAGGCGTTAACTATTGCCAGCCAGTTACGTTGTCCGCAGTGCCAGAATCAGAACTTACTGG
AATCCAATGCGCCGGTGGCGGTCAGTATGCGCCATCAGGTTTACAGCATGGTGGAAGAGGGGAAAAGTGAAGTCGAAATC
ATTGGCTGGATGACCGAACGCTACGGAGATTTTGTTCGCTATAACCCACCGTTAACGGGTCAGACGCTGGTGTTGTGGGC
GCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGATGGCGAAGCGATGA

Upstream 100 bases:

>100_bases
TTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGG
ATGGCGGGGGAGGAAGCGCG

Downstream 100 bases:

>100_bases
AACAGCCTCAAATACCGGTGAAAATGCTGACAACACTCACGATTTTGATGGTATTTCTCTGTGTCGGCAGTTATCTGCTA
AGTCCAAAATGGCAGGCGGT

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALTIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVEEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVMAKR

Sequences:

>Translated_127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALTIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVEEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVMAKR
>Mature_127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALTIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVEEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVMAKR

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1790512, Length=127, Percent_Identity=96.0629921259843, Blast_Score=186, Evalue=3e-49,
Organism=Escherichia coli, GI1788522, Length=117, Percent_Identity=40.1709401709402, Blast_Score=108, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616
- InterPro:   IPR017565 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 14535; Mature: 14535

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALTIASQLRCPQCQNQNLLESNAPVA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
WRVMAKR
HHHHHCC
>Mature Secondary Structure
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALTIASQLRCPQCQNQNLLESNAPVA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
WRVMAKR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]