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The map label for this gene is nrfB

Identifier: 218702737

GI number: 218702737

Start: 4698178

End: 4698744

Strand: Direct

Name: nrfB

Synonym: ECIAI39_4493

Alternate gene names: 218702737

Gene position: 4698178-4698744 (Clockwise)

Preceding gene: 218702736

Following gene: 218702738

Centisome position: 91.55

GC content: 54.14

Gene sequence:

>567_bases
ATGAGCGTATTACGTTCGTTATTAACTGCCGGGGTGCTGGCGTCGGGCCTGTTGTGGAGCCTGAACGGAATTACCGCTAC
CCCTGCGGCGCAGGCATCTGGTGATCGCTATGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATA
AACCAGATACCGAAGGTATGCATGGCAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGTACCAAC
TGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGG
AGAGCAGAACAGCGTCTGTATGTCCTGCCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAA
CCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATT
AAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCA
GCCATGA

Upstream 100 bases:

>100_bases
GGTGATCCCGCAGTGGGAAGAGCAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCCGTTCCGCCTCGCAAGGGGCG
GAAAACACAATGGAGTGAAT

Downstream 100 bases:

>100_bases
CCTGGTCACGTCGCCAGTTTCTCACCGGCGTCGGTGTGCTGGCCGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAA
ACGTTGAATATCAATGGTGT

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP

Sequences:

>Translated_188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP
>Mature_187_residues
SVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNC
HGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIK
ICVDCHSDQRTNPNFNPASVPLLKEQP

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=188, Percent_Identity=99.468085106383, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20657; Mature: 20525

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRNPDAACLDCHKPDTEGM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC
>Mature Secondary Structure 
SVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRNPDAACLDCHKPDTEGM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]