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The map label for this gene is nrfC

Identifier: 218702738

GI number: 218702738

Start: 4698741

End: 4699412

Strand: Direct

Name: nrfC

Synonym: ECIAI39_4494

Alternate gene names: 218702738

Gene position: 4698741-4699412 (Clockwise)

Preceding gene: 218702737

Following gene: 218702739

Centisome position: 91.56

GC content: 57.74

Gene sequence:

>672_bases
ATGACCTGGTCACGTCGCCAGTTTCTCACCGGCGTCGGTGTGCTGGCCGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGC
GAAAACGTTGAATATCAATGGTGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGG
ATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACAATTATTCGTAGCGAGCCGCAGGGCGAATTT
CCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCGTGCGCCGTGCGTTGACGTCTGCCCGACCGG
TGCGTCTTTTCGCGATGCCGCCAGCGGGATTGTCGATGTAAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCG
CCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACAGCGGACAAATGCGATTTCTGCCGTAAGACCAATTTG
CAGGCCGGTAAGCTGCCCGCGTGCGTTGAGGCTTGCCCGACGAAGGCGCTGACGTTTGGCAATCTGGACGATCCTAACAG
TGAGATTTCGCAACTGCTGCACCAGAAGCCTACTTACCGCTACAAGCTGGCGCTGGGAACCAAACCGAAGCTGTACCGCG
TACCGTTTAAATACGGGGAGGTGAGCCAATGA

Upstream 100 bases:

>100_bases
GTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTT
CCGTTGCTTAAGGAGCAGCC

Downstream 100 bases:

>100_bases
CGCAGACTTCCGCATTTCATTTTGAATCACTGGTGTGGGACTGGCCGATTGCCATCTACCTGTTTTTGATTGGTATTTCT
GCCGGTCTGGTGACGCTGGC

Product: formate-dependent nitrite reductase, 4Fe4S subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEF
PDVKYRFFRKSCQHCDRAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
QAGKLPACVEACPTKALTFGNLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKYGEVSQ

Sequences:

>Translated_223_residues
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEF
PDVKYRFFRKSCQHCDRAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
QAGKLPACVEACPTKALTFGNLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
>Mature_222_residues
TWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFP
DVKYRFFRKSCQHCDRAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQ
AGKLPACVEACPTKALTFGNLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKYGEVSQ

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=223, Percent_Identity=99.1031390134529, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI226510944, Length=213, Percent_Identity=50.2347417840376, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789370, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787872, Length=188, Percent_Identity=33.5106382978723, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787122, Length=188, Percent_Identity=33.5106382978723, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787749, Length=170, Percent_Identity=30.5882352941176, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790326, Length=178, Percent_Identity=29.7752808988764, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789067, Length=154, Percent_Identity=33.7662337662338, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=31.7365269461078, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=161, Percent_Identity=33.5403726708075, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787740, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789079, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787478, Length=104, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082179, Length=138, Percent_Identity=31.8840579710145, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367245, Length=158, Percent_Identity=32.2784810126582, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787963, Length=175, Percent_Identity=27.4285714285714, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24568; Mature: 24436

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVN
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHH
KVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDRAPCVDVCPTGASFRDAASGIVDV
HHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEC
NPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQAGKLPACVEACPTKALTFG
CCCHHHHHHHHHHHCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEC
NLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVN
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHH
KVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDRAPCVDVCPTGASFRDAASGIVDV
HHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEC
NPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQAGKLPACVEACPTKALTFG
CCCHHHHHHHHHHHCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEC
NLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]