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The map label for this gene is zraS

Identifier: 218702638

GI number: 218702638

Start: 4587103

End: 4588479

Strand: Direct

Name: zraS

Synonym: ECIAI39_4393

Alternate gene names: 218702638

Gene position: 4587103-4588479 (Clockwise)

Preceding gene: 218702636

Following gene: 218702639

Centisome position: 89.38

GC content: 54.61

Gene sequence:

>1377_bases
ATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGT
GGGGCTGTTTGCGGTGACAGTGATTCGCGATTATGGGCGCGAGACTGCCGCCGCCAGACAAACGCTGCTGGAAAAAGGCA
GTGTACTTATCCGTGCTCTCGAATCCGGCTCGCGCGTCGGCATGGGGATGCGCATGCATCATGCGCAGCAGCAGGCATTA
CTGGAAGAAATGGCCGGGCAGCCTGGTGTACGTTGGTTTGCGGTCACGGATGAACAAGGAACAATCGTGATGCATAGCAA
CTCCGGCATGGTGGGAAAACAGCTTTATTCCCCGCAGGAAATGCAGCAGTTACTTCCGGGAGATGAAGAAGCGTGGCGGC
GGATCGATAGCGCAGACGGCGAGCCTGTTCTGGAAATTTATCGCCAGTTTCAACCGATGTTTGCTGCTGGAATGCACCGG
ATGCGCCATATGCAGCAATATGCCGCGAAACCACAAGCAATTTTCATCGCTTTCGACGCCAGTAATATTGTGAGTGCCGA
AGATCGTGAGCAGAGAAACACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTGTTGTCATTCTTCT
GGTATCGTCGCTATCTGCGCTCGCGCCAGTTGTTGCAGGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCAT
CTTGCGGCAGGTGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGGCTGGCGAAATACTTTGCCGAACGCGC
GCCAGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAAGCTGACCGTTTAAACCGCGTGGTAAGCGAGT
TGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGAGCTCAACACGCTGATTAGCCACTCATTACAGCTG
GTAAGCCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAATTCAGGCCGATCC
GGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGA
CGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGACAGCGGTAAGGGAATTGCGGCAGATCAGCTTGAAGCC
ATCTTCACCCCGTACTTCACCACCAAAGCCGAAGGCACCGGACTGGGGCTGGCGGTCGTGCATAATATTGTTGAACAACA
CGGTGGTACAATTCAGGTCGCAAGCCAGGAGGGAAAAGGCGCAACGTTCACCCTCTGGCTTCCGGTCAATATTACGCGTA
AGGACCCACAAGGATGA

Upstream 100 bases:

>100_bases
TCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGG
ATGAGCAGGCAAAGAAGAAG

Downstream 100 bases:

>100_bases
CGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGG
GGCTATAACGTCGCGCTGGC

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLLPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAAKPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQL
VSQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVNITRKDPQG

Sequences:

>Translated_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLLPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAAKPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQL
VSQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVNITRKDPQG
>Mature_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLLPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAAKPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQL
VSQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVNITRKDPQG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=465, Percent_Identity=84.9462365591398, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=42.5619834710744, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=29.7071129707113, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=29.3877551020408, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=26.1467889908257, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=27.6595744680851, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081816, Length=218, Percent_Identity=29.3577981651376, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=29.4642857142857, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=27.5555555555556, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=26.4840182648402, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=28.5067873303167, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082128, Length=226, Percent_Identity=25.2212389380531, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=24, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=30.0884955752212, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=203, Percent_Identity=28.0788177339901, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790567, Length=235, Percent_Identity=29.7872340425532, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50464; Mature: 50464

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLLPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAAKPQAIFIAFDA
HHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVELNTLISHSLQLVSQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASQEGKGATFTLWLPVNITRKDPQG
HHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLLPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAAKPQAIFIAFDA
HHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVELNTLISHSLQLVSQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASQEGKGATFTLWLPVNITRKDPQG
HHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]