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The map label for this gene is zraR

Identifier: 218702639

GI number: 218702639

Start: 4588476

End: 4589801

Strand: Direct

Name: zraR

Synonym: ECIAI39_4394

Alternate gene names: 218702639

Gene position: 4588476-4589801 (Clockwise)

Preceding gene: 218702638

Following gene: 218702644

Centisome position: 89.41

GC content: 56.71

Gene sequence:

>1326_bases
ATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGG
CTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGCTGGAGCAGGTGCGGGAGCGAGTTTTTGATCTTGTGC
TTTGCGATGTGCGAATGGCGGAGATGGACGGCATTGCCACGCTAAAAGAGATCAAAGCGTTAAATCCGGCGATTCCGGTA
CTGATTATGACCGCATACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACCGGGGCGCTGGATTATCTCATCAAGCC
GCTGGATTTCGATAACCTGCAGGCGACGCTGGAAAAGGCGCTCGCGCATACGCACAGTATTGACGCTGAAACGCCTGCGG
TGTCTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA
TCGGAAGCCACGGTGCTGATCCACGGCGATTCCGGCACCGGTAAAGAGCTGGTCGCCAGGGCGATTCACGCCAGTAGCGC
ACGGAGTGAAAAACCACTGGTAACGCTCAACTGTGCGGCACTCAACGAATCCTTGCTGGAATCTGAATTGTTCGGTCACG
AAAAAGGGGCGTTTACCGGGGCCGACAAACGGCGGGAGGGGCGCTTTGTTGAGGCGGACGGCGGCACGTTGTTTCTCGAT
GAAATTGGCGATATCTCGCCGATGATGCAGGTGCGTCTGCTACGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGCAG
CAACCAGACCATCTCGGTTGATGTCCGGCTGATTGCGGCGACTCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTT
TTCGCCAGGATCTCTACTATCGCCTGAACGTGGTGGCGATTGAAGTTCCGTCGCTGCGTCAGCGGCGGGAAGATATTCCT
CTGCTGACAAACCATTTTCTTCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA
TCTGCTGATTCACTACGACTGGCCGGGAAATATTCGTGAACTGGAAAACGCGGTGGAACGGGCGGTGGTGCTGCTGACCG
GGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCGAGTACGCCGATCCCGCTGGTACAAAGTCAGGATATTCAG
CCGCTGGTGGAAGTCGAAAAAGAGGTGATTCTTGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCA
GTTAGGGATCACGCGCAAAACGCTCTTGGCAAAACTGTCGCGTTAG

Upstream 100 bases:

>100_bases
AACAACACGGTGGTACAATTCAGGTCGCAAGCCAGGAGGGAAAAGGCGCAACGTTCACCCTCTGGCTTCCGGTCAATATT
ACGCGTAAGGACCCACAAGG

Downstream 100 bases:

>100_bases
TTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAATGGATATCGGTCATTAAGGC
ATAAGCGCGTTTCTGCGCTT

Product: transcriptional regulatory protein ZraR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLTNHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLVQSQDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Sequences:

>Translated_441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLTNHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLVQSQDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR
>Mature_440_residues
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVL
IMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAPS
EATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDE
IGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPL
LTNHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLVQSQDIQP
LVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=98.6394557823129, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=447, Percent_Identity=48.7695749440716, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1788905, Length=445, Percent_Identity=44.4943820224719, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1790299, Length=462, Percent_Identity=41.7748917748918, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=48.2539682539683, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1789087, Length=313, Percent_Identity=46.3258785942492, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082152, Length=310, Percent_Identity=47.0967741935484, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=42.4528301886792, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=42.8134556574924, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=42.8571428571429, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1787583, Length=234, Percent_Identity=43.1623931623932, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=38.5542168674699, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87081858, Length=287, Percent_Identity=33.4494773519164, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=33.9285714285714, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=31.2977099236641, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=34.7826086956522, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48526; Mature: 48395

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMA
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLTNHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLVQSQDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLTNHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLVQSQDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]