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The map label for this gene is mdtL

Identifier: 218702560

GI number: 218702560

Start: 4491953

End: 4493128

Strand: Direct

Name: mdtL

Synonym: ECIAI39_4314

Alternate gene names: 218702560

Gene position: 4491953-4493128 (Clockwise)

Preceding gene: 218702559

Following gene: 218702561

Centisome position: 87.53

GC content: 53.66

Gene sequence:

>1176_bases
ATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACC
GCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTTTATCTGGCGGGCATGGCAGCTG
CGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCTGTCGCCATACCCGGCGCGGCGCTATTTATTATC
GCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGGCGCTTTCTACAGGGGCTGGGCGCGGGCTG
TTGTTACGTGGTGGCGTTCGCCATTTTACGCGACACACTGGATGATCGACGTCGGGCAAAGGTACTGTCGCTACTCAACG
GCATTACCTGCATCATTCCGGTGTTAGCGCCGGTGCTCGGACACCTGATTATGCTTAAATTCCCTTGGCAGAGTCTGTTC
TGGACGATGGCAACGATGGGCATCGCGATACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCTGCGGCCCCCGC
AGCTTCTGACAAACCACGAGAAAATAGCGAGTCACTGCTTAACCGGTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCA
GCGTTTCGGTGATCCTCACCTTCGTCAACACGTCGCCGGTATTGCTGATGGAAATCATGGGTTTTGAGCGCGGAGAATAC
GCCACCATTATAGCGTTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCGTTTGCGCTGGGAATTTTTAAGCC
ACGTACGTTGATGATCACCTCACAGGTGTTATTCCTGGCGGCGGGGATCACCCTTGCCGTTTCACCTTCCCATGCGGTTT
CGCTGTTTGGTATCACGCTGATTTGCGCCGGCTTCTCGGTAGGTTTTGGCGTAGCGATGAGTCAGGCGTTAGGGCCGTTT
TCATTACGCGCGGGCGTAGCCAGCTCGACCTTAGGTATTGCGCAAGTTTGCGGTTCGTCACTGTGGATTTGGCTGGCGGC
GGTGGTTGGTATCGGCGCATGGAATATGCTGATCGGGATTCTGATTGCCTGTAGCATAGTGAGCCTGTTGCTGATTATGT
TCATCGCGCCTGGACGCCCGGTTGCCGCTCATGAAGAAATCCATCACCACGCTTGA

Upstream 100 bases:

>100_bases
CTTGCCTGTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATTCTCCTTCACCACGTATGCTTTGCGTCACCTTG
CTATCAGGACGCTTTAGCCC

Downstream 100 bases:

>100_bases
TCTCAATCTGCTGCTCTGTCTGCAACTGCTGATGCAGGAGCGTAGCGTGACCAAAGCGGCGAAGCGAATGAACGTGACCC
CTTCGGCGGTGAGTAAGTCG

Product: multidrug efflux system protein MdtL

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WTMATMGIAILMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIIALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFIAPGRPVAAHEEIHHHA

Sequences:

>Translated_391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WTMATMGIAILMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIIALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFIAPGRPVAAHEEIHHHA
>Mature_390_residues
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIA
SVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFW
TMATMGIAILMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEYA
TIIALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFS
LRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFIAPGRPVAAHEEIHHHA

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=98.7212276214834, Blast_Score=767, Evalue=0.0,
Organism=Escherichia coli, GI1788509, Length=226, Percent_Identity=30.9734513274336, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI48994889, Length=242, Percent_Identity=29.3388429752066, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790794, Length=227, Percent_Identity=28.6343612334802, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787065, Length=168, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081738, Length=140, Percent_Identity=35, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41500; Mature: 41369

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWTMATMGIAILMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCE
ATIIALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITL
EEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFIAPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWTMATMGIAILMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCE
ATIIALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITL
EEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFIAPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA