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The map label for this gene is qacE [H]

Identifier: 218702461

GI number: 218702461

Start: 4388627

End: 4389028

Strand: Direct

Name: qacE [H]

Synonym: ECIAI39_4216

Alternate gene names: 218702461

Gene position: 4388627-4389028 (Clockwise)

Preceding gene: 218702460

Following gene: 218702462

Centisome position: 85.51

GC content: 44.53

Gene sequence:

>402_bases
ATGTCTTCGAAAACAAAATGCTGGCTATGGATGTTACTGGTCATCCTTTCTGAAACCTCTGCAACATCCACACTTAAAAT
GTTCGATAACAGTGAGGGGATGACAAAAACGCTGCTGCTGGCCCTAATCGTCGTACTGTATTGCATTTGTTACTACTCGC
TTTCACGGGCAGTAAAAGACATCCCCGTTGGTCTGGCTTACGCCACATGGTCCGGTACTGGCATTTTGATGGTTTCAACC
CTTGGGATTTTATTTTACGGTCAACACCCGGATACCGCCGCCATTATTGGTATGGTCATCATAGCCAGCGGTATTATCAT
TATGAATCTGTTCTCAAAAATGGGCAGTGAAGAGGCGGAAGAAACTCCAGTTACCAACCTCGATAAAAAAATCGCTAACT
GA

Upstream 100 bases:

>100_bases
TGGCAAAATCACCCGACGCCGTACTGACCGATGCTGCGGTCGATTTTTTGAAAGCACAGAAAAAATAACCTTACCTCGAT
AACAAACCTGGAGTTTAGAT

Downstream 100 bases:

>100_bases
CAAAAGGAATTGCTCATGTTTAATATTGGATTTTTATGGCTGGCACTTTCCATCGGTTCTGAAATAACCGGTACGTCAAT
GATCAAAAAAACCAATGGTT

Product: putative membrane transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Quaternary ammonium determinant E [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVVLYCICYYSLSRAVKDIPVGLAYATWSGTGILMVST
LGILFYGQHPDTAAIIGMVIIASGIIIMNLFSKMGSEEAEETPVTNLDKKIAN

Sequences:

>Translated_133_residues
MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVVLYCICYYSLSRAVKDIPVGLAYATWSGTGILMVST
LGILFYGQHPDTAAIIGMVIIASGIIIMNLFSKMGSEEAEETPVTNLDKKIAN
>Mature_132_residues
SSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVVLYCICYYSLSRAVKDIPVGLAYATWSGTGILMVSTL
GILFYGQHPDTAAIIGMVIIASGIIIMNLFSKMGSEEAEETPVTNLDKKIAN

Specific function: Multidrug exporter. Is implicated for the resistance to bacteriocidal quaternary ammonium compounds [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=99, Percent_Identity=40.4040404040404, Blast_Score=77, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 14493; Mature: 14362

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVVLYCICYYSLSRAVKD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPVGLAYATWSGTGILMVSTLGILFYGQHPDTAAIIGMVIIASGIIIMNLFSKMGSEEAE
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ETPVTNLDKKIAN
CCCCHHHHHHHCC
>Mature Secondary Structure 
SSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVVLYCICYYSLSRAVKD
CCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPVGLAYATWSGTGILMVSTLGILFYGQHPDTAAIIGMVIIASGIIIMNLFSKMGSEEAE
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ETPVTNLDKKIAN
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8494372 [H]