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The map label for this gene is nikC

Identifier: 218702223

GI number: 218702223

Start: 4117174

End: 4118007

Strand: Direct

Name: nikC

Synonym: ECIAI39_3959

Alternate gene names: 218702223

Gene position: 4117174-4118007 (Clockwise)

Preceding gene: 218702222

Following gene: 218702224

Centisome position: 80.22

GC content: 60.91

Gene sequence:

>834_bases
GTGAACTTTTTCCTCTCCTCCCGCTGGTCGGTTCGCCTGGCGCTGATCATTATCGCCCTGCTGGCACTGATTGCGCTCAC
CAGCCAGTGGTGGCTGCCGTATGACCCTCAGGCGATTGATCTGCCGTCGCGCCTGCTTTCACCGGATGCGCAGCACTGGC
TGGGTACCGATCATTTAGGGCGCGATATTTTCTCGCGGCTGATGGCGGCGACCCGCGTGTCGCTCGGCTCGGTAATGGCC
TGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGACTGATTGGCGGGCGCGTTGATCAGGCCAC
CATGCGCGTCGCGGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGC
TGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTG
CGCCAGCGCGAGTTTGTACTGGCATCACGGCTTTCTGGCGCGGGCCATGTGCGAGTGTTTGTAGATCATCTGGCAGGTGC
GGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTACACGTCGCGGGGATGTCCTTCCTCG
GCCTCGGCGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGACCCAGCCGCTG
CAAATGTTCTGGCCGGGGCTGGCGCTGTTTATCAGCGTGATGGCCTTTAACCTGGTGGGTGACGCACTGCGCGATCATCT
GGACCCTCATCTGGTGACGGAGCACGCACACTAA

Upstream 100 bases:

>100_bases
CGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGTATTCGT
CGTCATGAAGGAGCCCACGC

Downstream 100 bases:

>100_bases
TGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGTGTATCGTTAACCCTTAAA
CGCGGGCGTGTGCTGGCGTT

Product: nickel transporter permease NikC

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSRLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH

Sequences:

>Translated_277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSRLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH
>Mature_277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSRLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1789889, Length=277, Percent_Identity=100, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1787054, Length=259, Percent_Identity=38.2239382239382, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1787760, Length=255, Percent_Identity=36.8627450980392, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1789964, Length=257, Percent_Identity=37.7431906614786, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787498, Length=259, Percent_Identity=30.5019305019305, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787549, Length=264, Percent_Identity=29.9242424242424, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788505, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIKC_ECO57 (P0AFB0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86018
- PIR:   A91172
- RefSeq:   NP_290044.1
- RefSeq:   NP_312372.1
- ProteinModelPortal:   P0AFB0
- EnsemblBacteria:   EBESCT00000024785
- EnsemblBacteria:   EBESCT00000058697
- GeneID:   915805
- GeneID:   961234
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4870
- KEGG:   ecs:ECs4345
- GeneTree:   EBGT00050000010100
- HOGENOM:   HBG729429
- OMA:   MMLHVSG
- ProtClustDB:   PRK10417
- BioCyc:   ECOL83334:ECS4345-MONOMER
- InterPro:   IPR000515
- InterPro:   IPR014157
- TIGRFAMs:   TIGR02790

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 30362; Mature: 30362

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2580c874)-; HASH(0x2156d900)-; HASH(0x25bb6874)-; HASH(0x25fa5284)-; HASH(0x25d2d7a0)-; HASH(0x25c8bef4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCH
>Mature Secondary Structure
MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSRLLSPDAQHWLGTDHLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHCCCHHHH
RDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISLRQREFVLASRLSGAGHVRVF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
VDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796