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The map label for this gene is nikB

Identifier: 218702222

GI number: 218702222

Start: 4116233

End: 4117177

Strand: Direct

Name: nikB

Synonym: ECIAI39_3958

Alternate gene names: 218702222

Gene position: 4116233-4117177 (Clockwise)

Preceding gene: 218702221

Following gene: 218702223

Centisome position: 80.21

GC content: 58.94

Gene sequence:

>945_bases
ATGTTGCGTTATGTATTACGCCGCTTTCTGCTGCTGATCCCGATGGTGCTCGCCGCCTCGGTGATCATTTTTCTGATGCT
GCGCCTCGGCACCGGCGACCCGGCGCTCGATTATCTGCGTCTGTCTAACCTGCCGCCGACGCCGGAAATGCTGGCTTCTA
CCCGCACCATGCTGGGGCTGGATCAGCCGTTGTACGTGCAGTACGGAACCTGGTTGTGGAAAGCGCTGCATCTTGACTTT
GGTATCTCATTCGCCAGCCAACGCCCGGTGCTGGACGATATGCTGACCTTCCTGCCCGCCACGCTGGAGCTGGCGGGTGC
GGCGCTGGTATTAATTCTGCTCACTTCCGTGCCGCTCGGCATCTGGGCGGCGCGCCATCGCGACCGCCTGCCGGATTTCG
CCGTGCGTTTTATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGTTGGCGTTTTTACTGGTGATGGCATTTTCAGTG
TATCTGCAATGGCTGCCCGCGATGGGTTACGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATCGCTTTTATGTC
GCTGGCGATTAACGCGCGTCTGCTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCATGTGACCTGGGCGCGACTGC
GCGGCCTGAACGACAAACAGACCGAACGCCGCCACATCCTGCGCAATGCCTCGCTGCCGATGATCACCGCCGTGGGGATG
CACATTGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTC
GGCGATTTTCAACCGTGACTATCCGGTGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGA
TTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGTATTCGTCGTCATGAAGGAGCCCACGCGTGA

Upstream 100 bases:

>100_bases
TCTCAATGATGGTGGTTTCAAAACCGGAACTGGGTAACATCCCCTACGCGCCGATCGCCACCGAAATCCCGTTCGAACAG
ATTAAACCGGTGAAACCTTA

Downstream 100 bases:

>100_bases
ACTTTTTCCTCTCCTCCCGCTGGTCGGTTCGCCTGGCGCTGATCATTATCGCCCTGCTGGCACTGATTGCGCTCACCAGC
CAGTGGTGGCTGCCGTATGA

Product: nickel transporter permease NikB

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNLPPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDF
GISFASQRPVLDDMLTFLPATLELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLNDKQTERRHILRNASLPMITAVGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA

Sequences:

>Translated_314_residues
MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNLPPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDF
GISFASQRPVLDDMLTFLPATLELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLNDKQTERRHILRNASLPMITAVGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA
>Mature_314_residues
MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNLPPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDF
GISFASQRPVLDDMLTFLPATLELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLNDKQTERRHILRNASLPMITAVGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=99.6815286624204, Blast_Score=625, Evalue=0.0,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=40.5844155844156, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=32.8445747800587, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=34.5161290322581, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787761, Length=334, Percent_Identity=36.2275449101796, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787550, Length=275, Percent_Identity=30.5454545454545, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788504, Length=359, Percent_Identity=25.9052924791086, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35236; Mature: 35236

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNLPPTPEMLASTRTMLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
DQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLTFLPATLELAGAALVLILLTSVPLG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
IWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSVYLQWLPAMGYGGWQHIILPA
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLNDKQTERRHILRNASLPMITAVGM
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVVVFVVCNLIVDLLNA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDPRIRRHEGAHA
HHCCHHHHCCCCCC
>Mature Secondary Structure
MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNLPPTPEMLASTRTMLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
DQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLTFLPATLELAGAALVLILLTSVPLG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
IWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSVYLQWLPAMGYGGWQHIILPA
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLNDKQTERRHILRNASLPMITAVGM
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVVVFVVCNLIVDLLNA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDPRIRRHEGAHA
HHCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]