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The map label for this gene is iucA

Identifier: 218701706

GI number: 218701706

Start: 3569110

End: 3570900

Strand: Direct

Name: iucA

Synonym: ECIAI39_3423

Alternate gene names: 218701706

Gene position: 3569110-3570900 (Clockwise)

Preceding gene: 218701704

Following gene: 218701707

Centisome position: 69.55

GC content: 53.6

Gene sequence:

>1791_bases
TTGTTGATAATGAGAATCATTATTGACATAATTGTTATTATTTTGCTGTGTGGGAGCTGTTTGACTATGACCCTGCCCTC
TGAAAAACCAGCCACAGATGTGGCTGCGCAGTGCTTCCTGAATGCACTGATTCGTGAAACCACAGACTGGAAACTGACAG
AATACCCGCCAGACGAATTGCTCATCCCGCTGGATGAGCAGAAATCGCTCCATTTCAGAGTGGCTTATTTCTCCCCAACC
CAGCATCACCGCTTTGCATTTCCTGCACGTCTGGTCACGGCATCAGGCAGTTATCCTGTCGACTTTACCACTCTCTCCCG
GCTTATTATTGATAAGCTACGCCATCAACTGTTTCTGCCCGTTCCCCTCTGCGAAACTTTCCACCAGCGCGTGCTGGAAA
GCCATGTCCATACGCAACAGGCAATTGATGCCCGTCATGACTGGGCCGCTCTGCGTGAAAAAGCGTTGAATTTTGGCGAG
GCTGAGCAGGCGCTGCTGACAGGACACGCTTTCCACCCTGCGCCTAAGTCTCATGAACCGTTTAACCGGCGGGAGGCTGA
ACGCTACCTGCCAGATATGGCACCCCACTTCCCACTGCGGTGGTTTTCGGTGGATAAAACGCAAATCGCCGGTGAAAGTT
TGCATCTCAACCTTCAACAGCGGCTGACGCGATTTGCCGCAGAGAATGCGCCTCAGTTACTCAACGAATTAAGTGACAAC
CAATGGCTGTTCCCGTTGCACCCGTGGCAGGGGGAATATCTTTTGCAGCAGGGGTGGTGCCAGGCACTTGTTGCTAAAGG
GCTGATTAAAGACTTAGGTGAGGCCGGCACGTCGTGGCTGCCGACCACCTCTTCCCGTTCCCTCTACTGTGCCACCAGCC
GCGATATGATCAAGTTCTCCCTGAGCGTACGGCTGACCAACTCCATCCGTACTCTGTCCGTGAAAGAAGTGAAGCGTGGG
ATGCGCCTGGCACGCCTGGCTCAAACCGACGGCTGGCAGATGCTACAGGTCCGCTTCCCGACTTTCCGGGTAATGCAGGA
GGATGGCTGGGCCGGGCTGCGCGATCTTAACGGCAACATCATGCAGGAAAGTCTGTTTGCCCTGCGTGAAAATCTGCTGG
TGGATCAGCCGAAAAGCCAGACTAACGTACTGGTCTCCCTGACTCAGGCCGCACCTGATGGCGGTGATTCGCTGCTGGTT
TCGGCGGTAAAACGCCTGAGCGATCGCCTCGGTATCACTGTGCAACAGGCCGCCCATGCATGGGTCGATGCATACTGTCA
GCAAGTGCTAAAGCCGCTGTTTACGGCTGAAGCGGATTACGGCCTGGTGCTGCTGGCGCATCAGCAAAATATTCTTGTCC
AGATGCTTGGGGATCTGCCGGTCGGATTTATTTACCGTGACTGTCAGGGCAGCGCTTTTATGCCTCACGCGACAGACTGG
CTCGATTCTATTGGCGAGGCGCAGGCGGAAAATATTTTCACCCATGAGCAGTTGCTGCGCTATTTCCCTTATTACCTGCT
GGTTAACTCCACTTTTGCTGTGACCGCTGCGCTGGGGGCTGCCGGGCTGGACAGCGAATCGAATCTGATGGCTCGTGTAC
GAGCATCGCTGGCTGAAGTGCGTGATCAGGTGACTCATAAAACCTGTCTCAACTACGTGCTGGAAAGTCCGTACTGGAAC
GTAAAAGGTAACTTTTTCTGTTATCTGAACGATCATAACGAGAACACCATTGTTGACCCTTCGGTGATCTATTTCGATTT
CGCTAACCCGCTGCAGGCTCAGGAGGTCTGA

Upstream 100 bases:

>100_bases
TTTGGGGCGTATCTTCGATCCCTGGTGACATTATAATGTTTCAACTCCATGTATTAATTGTGTTTATTTGTAAAATTAAT
TTATCTGACAATAACATTTA

Downstream 100 bases:

>100_bases
ATGTCTGAGGCAAACATTATTCACAGCAGATATGGACTGCGCTGTGAAAAACTCGACAAGCCCCTGAATCTTGGCTGGGG
ACTGGACAATAGCGCGGTGT

Product: aerobactin siderophore biosynthesis protein

Products: ADP; phosphate; aerobactin

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MLIMRIIIDIIVIILLCGSCLTMTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQKSLHFRVAYFSPT
QHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGE
AEQALLTGHAFHPAPKSHEPFNRREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSIRTLSVKEVKRG
MRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLV
SAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDW
LDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEVRDQVTHKTCLNYVLESPYWN
VKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV

Sequences:

>Translated_596_residues
MLIMRIIIDIIVIILLCGSCLTMTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQKSLHFRVAYFSPT
QHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGE
AEQALLTGHAFHPAPKSHEPFNRREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSIRTLSVKEVKRG
MRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLV
SAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDW
LDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEVRDQVTHKTCLNYVLESPYWN
VKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV
>Mature_596_residues
MLIMRIIIDIIVIILLCGSCLTMTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQKSLHFRVAYFSPT
QHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGE
AEQALLTGHAFHPAPKSHEPFNRREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSIRTLSVKEVKRG
MRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLV
SAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDW
LDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEVRDQVTHKTCLNYVLESPYWN
VKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV

Specific function: Unknown

COG id: COG4264

COG function: function code Q; Siderophore synthetase component

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iucA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007310
- InterPro:   IPR022770 [H]

Pfam domain/function: PF06276 FhuF; PF04183 IucA_IucC [H]

EC number: 6.3.2.27

Molecular weight: Translated: 67504; Mature: 67504

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIMRIIIDIIVIILLCGSCLTMTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDEL
CHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCE
LIPLDEQKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLP
EEECCCCCCEEEEEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECC
VPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
FNRREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
CCHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCC
QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFS
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEE
LSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNI
EEEEEECCHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHCCCHH
MQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHA
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHH
WVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDW
HHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHH
LDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV
HHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCCEEECCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MLIMRIIIDIIVIILLCGSCLTMTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDEL
CHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCE
LIPLDEQKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLP
EEECCCCCCEEEEEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECC
VPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
FNRREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
CCHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCC
QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFS
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEE
LSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNI
EEEEEECCHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHCCCHH
MQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHA
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHH
WVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDW
HHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHH
LDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV
HHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCCEEECCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; citrate; N6-acetyl-N6-hydroxylysine

Specific reaction: 4 ATP + citrate + N6-acetyl-N6-hydroxylysine = 4 ADP + 4 phosphate + aerobactin

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8003107 [H]