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The map label for this gene is ygfO

Identifier: 218701591

GI number: 218701591

Start: 3451457

End: 3452857

Strand: Direct

Name: ygfO

Synonym: ECIAI39_3297

Alternate gene names: 218701591

Gene position: 3451457-3452857 (Clockwise)

Preceding gene: 218701590

Following gene: 218701592

Centisome position: 67.25

GC content: 49.11

Gene sequence:

>1401_bases
ATGTCTGATATAAACCATGCAGGTTCTGACCTTATATTTGAACTGGAGGATCGCCCTCCCTTTCATCAGGCTCTCGTTGG
TGCCATTACCCATCTTTTGGCAATTTTCGTTCCGATGGTAACCCCCGCGTTAATCGTGGGTGCGGCCTTACAGCTTTCCG
CTGAAACAACCGCTTATCTGGTTTCTATGGCGATGATCGCTTCTGGTATTGGTACCTGGTTACAAGTAAACCGCTACGGC
ATCGTCGGTTCTGGTCTTCTCTCAATTCAGTCAGTCAATTTTTCATTTGTTACGGTCATGATTGCGCTGGGCAGCAGCAT
GAAAAGCGACGGTTTTCACGAAGAGTTAATCATGTCGTCGCTCCTCGGCGTCTCCTTCGTTGGCGCATTTCTGGTTGTCG
GCTCTTCTTTTATCCTGCCCTATTTACGTCGGGTTATTACGCCTACTGTCAGCGGTATTGTGGTGCTGATGATCGGCTTA
AGCCTGATTAAAGTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACA
TCTCGGCGTTGGTTTATTGGTTTTAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGA
TCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCG
TTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTCAGCTTCCATCAGTTCCTGGTGGTCGGCACGATTTATCT
GCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATC
AGTCCCGACTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACC
ACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCT
GGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGT
TGATGTTTTCCATGATTGCCATCGCTGGGATTCGCATCATCATCACCAACGGATTAAAGCGGCGTGAAACACTTATTGTC
GCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATT
AGTTGAAAACCCTATTTGTGCCGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAA
ACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAA

Upstream 100 bases:

>100_bases
AAATGAGATAAGCCGTTCTCAAGGCGTCCTGCGCCCCAAATTCGTGGGAATTTATTTTACTGATTCAGATGATCAAATTT
ACTTTAAAAGGAGTGAAGGG

Downstream 100 bases:

>100_bases
CAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGGTC
GATAACCCGGAAGAGATTGC

Product: putative transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYG
IVGSGLLSIQSVNFSFVTVMIALGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGYIASLCLGMVDFSSMRNLP
LITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLT
TFAQNNGVIQMTGVASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRETLIV
ATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGYRQENVLPGITSAEEMD

Sequences:

>Translated_466_residues
MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYG
IVGSGLLSIQSVNFSFVTVMIALGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGYIASLCLGMVDFSSMRNLP
LITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLT
TFAQNNGVIQMTGVASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRETLIV
ATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGYRQENVLPGITSAEEMD
>Mature_465_residues
SDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGI
VGSGLLSIQSVNFSFVTVMIALGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGLS
LIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGYIASLCLGMVDFSSMRNLPL
ITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTT
FAQNNGVIQMTGVASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRETLIVA
TSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGYRQENVLPGITSAEEMD

Specific function: Specific, proton motive force-dependent high-affinity transporter for xanthine

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Escherichia coli, GI87082178, Length=466, Percent_Identity=100, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI1790087, Length=444, Percent_Identity=45.045045045045, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87082181, Length=465, Percent_Identity=26.8817204301075, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081818, Length=407, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788843, Length=409, Percent_Identity=25.9168704156479, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=497, Percent_Identity=24.9496981891348, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17542260, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17541904, Length=273, Percent_Identity=25.2747252747253, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XANQ_ECO57 (P67446)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91098
- PIR:   G85943
- RefSeq:   NP_289452.1
- RefSeq:   NP_311782.1
- ProteinModelPortal:   P67446
- EnsemblBacteria:   EBESCT00000027090
- EnsemblBacteria:   EBESCT00000056125
- GeneID:   916416
- GeneID:   960724
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4221
- KEGG:   ecs:ECs3755
- GeneTree:   EBGT00050000009612
- HOGENOM:   HBG470187
- OMA:   HEELIVS
- ProtClustDB:   CLSK877814
- BioCyc:   ECOL83334:ECS3755-MONOMER
- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043
- PANTHER:   PTHR11119:SF3
- PANTHER:   PTHR11119
- TIGRFAMs:   TIGR00801
- TIGRFAMs:   TIGR03173

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 49109; Mature: 48978

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x24e9b878)-; HASH(0x229d72e0)-; HASH(0x25034708)-; HASH(0x2502ba4c)-; HASH(0x244ce8e0)-; HASH(0x25043f50)-; HASH(0x24c77a7c)-; HASH(0x2504401c)-; HASH(0x24cd478c)-; HASH(0x24f56914)-; HASH(0x244d7374)-; HASH(0x25043efc)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQLSAETTAYL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
VSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIALGSSMKSDGFHEELIMSS
HHHHHHHHCCHHHHEEECCCEEECCHHEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHH
LLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGLSLIKVGIIDFGGGFAAKSSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGYIASLCLGMVDFSSMRNLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITATAMVSRRPIQGEEYQSRLKGG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
VLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGVASRYVGRTIAVMLVILGLFPMIGGFF
CHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRETLIVATSLGLGLGVSYDPEIFKIL
HHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHEEEEEEECCCCCCCCCCCHHHHHH
PASIYVLVENPICAGGLTAILLNIILPGGYRQENVLPGITSAEEMD
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCH
>Mature Secondary Structure 
SDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQLSAETTAYL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH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CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796