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The map label for this gene is guaD

Identifier: 218701592

GI number: 218701592

Start: 3452872

End: 3454191

Strand: Direct

Name: guaD

Synonym: ECIAI39_3298

Alternate gene names: 218701592

Gene position: 3452872-3454191 (Clockwise)

Preceding gene: 218701591

Following gene: 218701593

Centisome position: 67.28

GC content: 49.02

Gene sequence:

>1320_bases
ATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGGTCGATAACCCGGAAGA
GATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGG
AAAACGGAAAGCATCAAATTCCTGACACCATTCGCGTGCGCGACTATCGCGGCAAACTGATAGTACCGGGCTTTGTCGAT
ACACATATCCATTATCCGCAAAGTGAAATGGTGGGGGCCTATGGTGAGCAATTGCTGGAATGGTTGAATAAACACACCTT
CCCTACTGAACGTCGTTATGAGGATTTAGAGTACGCCCGCGAAATGTCGGCATTCTTCATCAAGCAGCTTTTACGTAACG
GAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCCGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAAT
ATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCA
CCAAAGCAAAGAACTGATCGAGCGCTGGCACAAAAATGGTCGTCTGTTGTATGCGATTACGCCACGCTTCGCCCCTACCT
CATCTCCTGAACAGATGGCGATGGCGCAACGCCTGAAAGAAGAATATCCGGATACGTGGGTACATACCCATCTCTGTGAA
AACAAAGATGAAATTGCCTGGGTGAAATCGCTTTATCCTGACCATGATGGTTATCTGGATGTTTACCATCAGTACGGCCT
GACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGCTCGTCTCAGCGAAACCAAATCCA
GCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTC
AAAGTGGGCATGGGAACGGATATCGGTGCCGGAACAACTTTCAACATGCTGCAAACGCTAAACGAAGCCTACAAAGTGTT
GCAACTACAAGGCTATCGCCTCTCGGCATATGAAGCATTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTG
ACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAG
CTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCG
CACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAA

Upstream 100 bases:

>100_bases
CGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATG
GATTAACAGTAAAGGAGTCA

Downstream 100 bases:

>100_bases
TAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAA
GGCGCGCTGGATAATTATTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVD
THIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHIN
MRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCE
NKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKV
KVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQ
LRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Sequences:

>Translated_439_residues
MMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVD
THIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHIN
MRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCE
NKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKV
KVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQ
LRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
>Mature_439_residues
MMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVD
THIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHIN
MRMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCE
NKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKV
KVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQ
LRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=448, Percent_Identity=34.8214285714286, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1789249, Length=439, Percent_Identity=99.5444191343963, Blast_Score=918, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=418, Percent_Identity=33.4928229665072, Blast_Score=219, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24643849, Length=397, Percent_Identity=34.0050377833753, Blast_Score=209, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50187; Mature: 50187

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQI
CCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCC
PDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAR
CCCEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLD
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEEEEEHEECCCCCCHHHH
TAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCE
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
NKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWARLSETKSSIAFCPT
CCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEECCC
SNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHEE
YLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLG
EEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHEEEEEC
DDRSIYRTYVDGRLVYERN
CCCHHHHEEECCEEEEECC
>Mature Secondary Structure
MMSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQI
CCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCC
PDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAR
CCCEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLD
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEEEEEHEECCCCCCHHHH
TAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCE
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
NKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWARLSETKSSIAFCPT
CCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEECCC
SNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHEE
YLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLG
EEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHEEEEEC
DDRSIYRTYVDGRLVYERN
CCCHHHHEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]