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The map label for this gene is lysR

Identifier: 218701554

GI number: 218701554

Start: 3407555

End: 3408490

Strand: Direct

Name: lysR

Synonym: ECIAI39_3259

Alternate gene names: 218701554

Gene position: 3407555-3408490 (Clockwise)

Preceding gene: 218701552

Following gene: 218701560

Centisome position: 66.4

GC content: 53.21

Gene sequence:

>936_bases
ATGGCCGCCGTTAACTTACGTCATATTGAAATTTTTCATGCGGTAATGACCGCCGGAAGTCTGACTGAGGCGGCACACCT
GCTACACACCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCA
TACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC
ATTGTCAGTGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCTCAATC
TTTTTTACCGCAGCTCCTGCAACCCTTTCTGGCACGTTATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGC
TACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGT
ACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGCCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGA
TGATTTTCACGGTGAAAACTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAATTGTTTACTGAGA
ATCAGGTTAAACGACGCATGATCGTAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGCGTT
TCGGTGGTTAACCCGCTCACCGCACTGGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGCATTGCGGTTCCATT
CACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTGCAGGCGTTTAGTGAGCATTTACAAGCGGGAT
TACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA

Upstream 100 bases:

>100_bases
TTTTTATGATTACGCCACATCATAAAAAGAATAAAAAATATCGATTTATGTCGAGTCTATGCAAAAATGATATGGATTAC
CGGATTGCGAGAGAGCGCTA

Downstream 100 bases:

>100_bases
AAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAGAACACTGCGCTCTTCTGGCACCAGTAAACCA
ATTTCTGTGCAGCCAAAAAT

Product: DNA-binding transcriptional regulator LysR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEKVIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDR
IVSAAESLREFRQGELSIACLPVFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKRRMIVETHSAASVCAMVRAGVGV
SVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLHRPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA

Sequences:

>Translated_311_residues
MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEKVIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDR
IVSAAESLREFRQGELSIACLPVFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKRRMIVETHSAASVCAMVRAGVGV
SVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLHRPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA
>Mature_310_residues
AAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEKVIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRI
VSAAESLREFRQGELSIACLPVFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTERT
ELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKRRMIVETHSAASVCAMVRAGVGVS
VVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLHRPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA

Specific function: This protein activates the transcription of the lysA gene encoding diaminopimelate decarboxylase. LysR is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789204, Length=311, Percent_Identity=98.3922829581994, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=28.695652173913, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI145693105, Length=268, Percent_Identity=23.8805970149254, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788887, Length=275, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787530, Length=262, Percent_Identity=26.3358778625954, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=25.3061224489796, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790262, Length=264, Percent_Identity=25.3787878787879, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=25.3289473684211, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081904, Length=185, Percent_Identity=28.6486486486486, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=26.6932270916335, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34423; Mature: 34292

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEKVIGLKLFERIRGRLHP
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
TVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLPVFSQSFLPQLLQPFLARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHC
PDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTERTELLSLDEVCVLPPGHPLAV
CCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHCCCCCEEECCCCCCHHH
KKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKRRMIVETHSAASVCAMVRAGVGV
HCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLHRPSSALVQAFSEHLQAGLPKLVTS
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
LDAILSSATTA
HHHHHHHCCCC
>Mature Secondary Structure 
AAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEKVIGLKLFERIRGRLHP
CCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
TVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLPVFSQSFLPQLLQPFLARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHC
PDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTERTELLSLDEVCVLPPGHPLAV
CCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHCCCCCEEECCCCCCHHH
KKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKRRMIVETHSAASVCAMVRAGVGV
HCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLHRPSSALVQAFSEHLQAGLPKLVTS
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
LDAILSSATTA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6350602; 9278503; 2836407 [H]