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The map label for this gene is galR

Identifier: 218701552

GI number: 218701552

Start: 3405133

End: 3406164

Strand: Direct

Name: galR

Synonym: ECIAI39_3257

Alternate gene names: 218701552

Gene position: 3405133-3406164 (Clockwise)

Preceding gene: 218701549

Following gene: 218701554

Centisome position: 66.35

GC content: 52.91

Gene sequence:

>1032_bases
ATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAA
AGCCAGCGAAGCTTCCCGGCTTGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC
TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCG
GTCGAACAGGTGGCTTATCACACTGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGC
CATTGAGCAACTGATCCGCCATCGCTGTGCTGCGCTGGTTGTCCATGCCAAAATGATCCCGGATGCCGATTTAGCCTCAT
TAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGAT
CGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTC
TATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTCCGGCCAATGACCGGCTGG
TGACATTTGGCGAACCAGACGAAAGCGGGGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGCAGAAATTTCACTGCG
GTAGCCTGTTATAACGATTCAATGGCGGCGGGCGCAATGGGCGTGCTCAATGATAATGGTATTGATGTACCGGGTGAGAT
TTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGA
CGATGGCGACGCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGT
CCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAA

Upstream 100 bases:

>100_bases
TGGCTCACATTCCCACGATGAAAGCATGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGT
TTACCCACTAAGGTATTTTC

Downstream 100 bases:

>100_bases
CCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAA
ACAGAACTTCTGGTAACAGC

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFS
PTLVRRHSVSTPSLEASHHATSD

Sequences:

>Translated_343_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFS
PTLVRRHSVSTPSLEASHHATSD
>Mature_342_residues
ATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAV
EQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDR
YGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGEPDESGGEQAMTELLGRGRNFTAV
ACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSP
TLVRRHSVSTPSLEASHHATSD

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=343, Percent_Identity=99.7084548104956, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=55.8912386706949, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=33.5311572700297, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1790369, Length=304, Percent_Identity=33.2236842105263, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=32.6347305389222, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=32.1428571428571, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=28.6144578313253, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786540, Length=354, Percent_Identity=27.1186440677966, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.25, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=25.077399380805, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=23.4527687296417, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790689, Length=319, Percent_Identity=24.7648902821317, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786268, Length=333, Percent_Identity=23.4234234234234, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37121; Mature: 36989

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTE
CCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEE
YLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD
HHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTE
CCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEE
YLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD
HHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]