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The map label for this gene is cysJ

Identifier: 218701248

GI number: 218701248

Start: 3049814

End: 3051613

Strand: Reverse

Name: cysJ

Synonym: ECIAI39_2946

Alternate gene names: 218701248

Gene position: 3051613-3049814 (Counterclockwise)

Preceding gene: 218701253

Following gene: 218701247

Centisome position: 59.46

GC content: 56.89

Gene sequence:

>1800_bases
ATGACGACACAGGTCCCACCTTCCGCGTTGCTTCCGTTGAACCCGGAGCAACTGGCACGCCTTCAGGCGGCCACGACCGA
TTTAACTCCCACCCAGCTTGCCTGGGTTTCTGGCTATTTCTGGGGCGTGCTCAACCAGCAGCCTGCTGCGCTTGCAGCGA
CGCCAGCGCCCGCCGCAGAAATGCCGGGTATAACTATTATCTCCGCCTCGCAAACCGGCAATGCGCGCCGGGTTGCTGAA
GCATTACGCGATGATTTATTGGCGGCAAAACTGAACGTTAAGCTGGTGAACGCGGGCGACTATAAATTCAAACAAATCGC
CAGCGAAAAACTGCTTATCGTGGTGACGTCAACGCAAGGGGAAGGGGAACCGCCGGAAGAAGCCGTCGCGCTGCATAAGT
TCCTGTTCTCCAAAAAAGCGCCGAAGCTGGAAAACACCGCATTTGCCGTGTTTAGCCTCGGCGATAGTTCTTATGAATTT
TTCTGCCAGTCCGGGAAAGATTTTGACAGCAAGCTGGCGGAACTGGGCGGTGAACGCCTGCTCGACCGTGTCGATGCCGA
TGTTGAATATCAGGCCGCTGCCAGCGAGTGGCGCGCCCGCGTGGTTGATGCGCTTAAATCGCGTGCGCCTGTCGCGGCAC
CTTCGCAATCCGTCGCTACTGGCGCGGTAAATGAAATCCACACCAGCCCGTACAGCAAAGACGCGCCGCTGGTGGCGAGC
CTCTCTGTTAACCAGAAAATTACCGGGCGTAACTCTGAAAAAGACGTTCGCCATATCGAAATTGACTTAGGTGACTCTGG
CCTGCGTTATCAGCCGGGTGACGCGCTGGGCGTCTGGTATCAGAATGATCCGGCACTGGCGAAAGAACTTGTCGAACTGC
TGTGGCTGAAAGGCGATGAACCTGTCACCGTTGACGGCAAAACGCTGACCCTGAGCGAAGCGCTACAGTGGCACTTTGAA
CTCACCGTCAACACCGCGAATATTGTTGAGAACTACGCCACGCTTACCCGCAGCGAAACGCTGCTGCCGCTGGTGGGCGA
TAAAGCGAAGTTACAGCATTACGCCGCGACGACGCCGATTGTTGACATGGTGCGTTTCTCTCCGGCACAGCTGGATGCCG
AAGCGCTGATTAATCTGCTGCGCCCGCTGACCCCGCGCCTGTATTCCATCGCCTCCTCGCAGGCGGAAGTCGAGAACGAA
GTACACGTCACCGTTGGCGTGGTGCGTTACGACGTGGAAGGCCGCGCCCGTGCCGGTGGTGCCTCCAGCTTCCTCGCGGA
CCGCGTGGAAGAAGAGGGCGAAGTCCGCGTATTTATCGAACATAACGACAACTTCCGCCTGCCAGCCAATCCAGAAACCC
CGGTGATTATGATTGGCCCAGGCACCGGCATCGCGCCGTTCCGCGCCTTTATGCAGCAGCGCGCCGCTGACGAAGCGCCG
GGTAAAAACTGGTTGTTCTTTGGCAACCCGCACTTTACGGAAGATTTCCTCTACCAGGTGGAGTGGCAGCGCTACGTCAA
AGAGGGCGTGCTGACACGTATCGATCTTGCCTGGTCGCGCGATCAAAAAGAAAAAGTTTACGTACAAGACAAACTGCGCG
AACAGGGCGCAGAGCTGTGGCGCTGGATCAATGACGGTGCCCACATTTATGTCTGCGGCGACGCTAATCGCATGGCGAAA
GACGTTGAACAGGCACTTCTGGAAGTGATTGCCGAATTTGGTGGCATGGACACCGAAGCGGCGGATGAATTTTTAAGTGA
GCTGCGCGTAGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
TTAATCCACACCGTTCACCCCGTTAACCTTACCTTCTCTTCTGTTTTATGGGCGCTGACAGGGCGCAGAAACAGCTTTGC
TTACTGGAACATAACGACGC

Downstream 100 bases:

>100_bases
TGAACGAAAAGCATCCAGGGCCTTTAGTGGTCGAAGGAAAACTGACAGACGCCGAGCGCATGAAGCTTGAGAGCAACTAC
CTGCGCGGGACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALAKELVELLWLKGDEPVTVDGKTLTLSEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALAKELVELLWLKGDEPVTVDGKTLTLSEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
>Mature_598_residues
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAEA
LRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFF
CQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALAKELVELLWLKGDEPVTVDGKTLTLSEALQWHFEL
TVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEV
HVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAPG
KNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKD
VEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=601, Percent_Identity=29.9500831946755, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=29.4807370184255, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=29.4807370184255, Blast_Score=223, Evalue=6e-58,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.6532663316583, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI221316705, Length=606, Percent_Identity=29.042904290429, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI10835173, Length=654, Percent_Identity=29.0519877675841, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI221316707, Length=527, Percent_Identity=29.6015180265655, Blast_Score=199, Evalue=8e-51,
Organism=Homo sapiens, GI40254422, Length=649, Percent_Identity=26.9645608628659, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=28.1464530892449, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI169790956, Length=437, Percent_Identity=28.1464530892449, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=98.9983305509182, Blast_Score=1215, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=28.9308176100629, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=583, Percent_Identity=26.0720411663808, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531441, Length=394, Percent_Identity=24.8730964467005, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=634, Percent_Identity=28.391167192429, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321143, Length=399, Percent_Identity=31.5789473684211, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325305, Length=626, Percent_Identity=23.961661341853, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137192, Length=624, Percent_Identity=28.0448717948718, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24582192, Length=510, Percent_Identity=29.8039215686275, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=32.0610687022901, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=32.0610687022901, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=32.0610687022901, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24660907, Length=600, Percent_Identity=25.1666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24660903, Length=600, Percent_Identity=25.1666666666667, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66201; Mature: 66070

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALAKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVDGKTLTLSEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPI
CEEECCCEEEHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALAKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVDGKTLTLSEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPI
CEEECCCEEEHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA