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The map label for this gene is ycdT

Identifier: 218700465

GI number: 218700465

Start: 2170044

End: 2171501

Strand: Reverse

Name: ycdT

Synonym: ECIAI39_2131

Alternate gene names: 218700465

Gene position: 2171501-2170044 (Counterclockwise)

Preceding gene: 218700470

Following gene: 218700464

Centisome position: 42.31

GC content: 38.13

Gene sequence:

>1458_bases
TTGTGGTGGATTTTTATATTGACAACAGTACAGTTCTTATATCTATTAATGACAGAAAGGGATCTACAACCTACAGATTG
GTGTAGCTTTATGGAAAAAGACTATTTGGGAATTAGTAGTACTGTATTAGTGAGTTTATTGTTTGGGCTTGCGTTGGTTT
TGGTGAATAGTTGGTTCAATCAGCCAACCGTTGAGGAAGTTGTTCCACGATCAACCTATCTGATGGTTATGATTGCTTTA
TTTTTTATCGATACTGTTGCATTTATTTTTATGCAGTTGTATTTCATTTATGACCGTAGGCAATTTTCAAACTGTATACT
TAGTTTGGCTTTTCTAAGTTGTTTGATTTACTTTATTAAAACCGTCATTATCATTCGGCAAATTATTGAGGGGCGTTTGA
CAAGCAGTGTTGTTCAAAATGACATTGCAATTTATTATTTGTTTCGTCAGATGAGTTTGTGCGTATTAATATTTCTGGCA
TTGGTAAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTGTTTTCCAAAAAAGTGACTTTGTGCATAACTTTGTTTTT
TGTTCTTGGGGGGCCGATAGTTGCTCACATATTTTCTAGCCATTATGAGAGCTACGATTTACACATTGCCGAGTTAACCA
ATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAAT
CTATACTTTAATGGTTTACGCTGTGATATCTGGAACGGGGTGTCGGTGATTGCTTTTTGTGCCGTGCTGTACAATATCTC
TCTGTTATTTATGAGCAGGTATAGCGTGTCCATTTGGTATATTAGTCGCACTATTGAAGTAGTCAGTAAGTTAACCGTTT
TGGTAATTTTTATGTGCCATATATTCAGTGCGCTACGAGTAACAAAGGACATTGCACATCGCGATCCCTTAACCAATATA
TTTAACAGAAATTATTTTTTTAATGAACTGACGGTTCAATCAGCATCGGCCAAAAAAACACCTTATTGCGTCATGATTAT
GGATATTGACCACTTCAAAAAAGTCAACGACACCTGGGGACATCCGGTTGGCGATCAAGTGATAAAGACAGTGGTGAATA
TCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTTGGCGGCGAAGAGTTTGGCGTCTTGCTGACGGAGATC
GATACTGAATGTGCGAAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAACGTTTAACTGGCGATAATCCTGAATACGC
CATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTAACGCAGAAAAATGAGTTAAACCCTAAGGAGATCTATCAAC
TGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTAGTAAAAGAGGCAGAAATTCTTATCAAC
CGCAAGGATGATGAATGA

Upstream 100 bases:

>100_bases
AACCATTTATATGTATTTGCAAGCCTGTCTTGTCGCGAAGAATGTATAAAGCACTAAGGTAATAATAATTGCGGTCTGCA
ACTATCATCATTGGTATTGT

Downstream 100 bases:

>100_bases
GTCTGTCGCCAGTTGTCGACCTCCGCTTGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACT
TTCGTACGAGCTCTTCTTTA

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MWWIFILTTVQFLYLLMTERDLQPTDWCSFMEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYLMVMIAL
FFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIRQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLA
LVNKVSENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVN
LYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNI
FNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEI
DTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
RKDDE

Sequences:

>Translated_485_residues
MWWIFILTTVQFLYLLMTERDLQPTDWCSFMEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYLMVMIAL
FFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIRQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLA
LVNKVSENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVN
LYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNI
FNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEI
DTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
RKDDE
>Mature_485_residues
MWWIFILTTVQFLYLLMTERDLQPTDWCSFMEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYLMVMIAL
FFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIRQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLA
LVNKVSENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVN
LYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNI
FNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEI
DTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
RKDDE

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=444, Percent_Identity=93.9189189189189, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1788085, Length=446, Percent_Identity=38.7892376681614, Blast_Score=304, Evalue=9e-84,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=37.5, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=34.1176470588235, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=34.9693251533742, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=31.4606741573034, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=195, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=32.7160493827161, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=30.952380952381, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=29.1139240506329, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=176, Percent_Identity=32.3863636363636, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55809; Mature: 55809

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWWIFILTTVQFLYLLMTERDLQPTDWCSFMEKDYLGISSTVLVSLLFGLALVLVNSWFN
CEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
QPTVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVIIIRQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVN
HHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
LYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCH
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHH
IFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWG
HHHHHHHHHHHHHCCCHHHHHCCCCEEHHHEEECCCCCCCCEEEEEEEHHHHHHCCCCCC
HPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLT
CCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHEEE
RKDDE
CCCCC
>Mature Secondary Structure
MWWIFILTTVQFLYLLMTERDLQPTDWCSFMEKDYLGISSTVLVSLLFGLALVLVNSWFN
CEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
QPTVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVIIIRQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVN
HHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
LYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCH
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHH
IFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWG
HHHHHHHHHHHHHCCCHHHHHCCCCEEHHHEEECCCCCCCCEEEEEEEHHHHHHCCCCCC
HPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLT
CCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHEEE
RKDDE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]