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The map label for this gene is ycdW

Identifier: 218700464

GI number: 218700464

Start: 2168680

End: 2169618

Strand: Reverse

Name: ycdW

Synonym: ECIAI39_2130

Alternate gene names: 218700464

Gene position: 2169618-2168680 (Counterclockwise)

Preceding gene: 218700465

Following gene: 218700463

Centisome position: 42.28

GC content: 51.22

Gene sequence:

>939_bases
ATGGATATCATCTTTTATCACCCAACGTTCGATACCCAATGGTGGATTGAGGTACTGCGCAAAGCTATTCCTCAGGCAAG
AGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCAGTTGAAATGCTGGCAG
GGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACATCCTGAAATG
CTGAAGCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGT
GCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTTCCTGAATATCATC
GGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCACAGAGTCTGCAAACCTGGCGCTTC
CCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTCTCTGCATT
TCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACACCTGCAACCGTCGGCATTATTAATCAACAATTACTCG
AAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTCGTGGAAGATGACCTGCTCGCGGCGCTG
GATAGCGGCAAAGTTAAAGGCGCAATGCTGGATGTTTTTAATCGTGAACCCTTACCGCCGGAAAGTCCGCTCTGGCAACA
TCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCTGTCCCGCTGAAGCTGTGGATTACATTTCGCGCACCATTG
CCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAA

Upstream 100 bases:

>100_bases
CTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCGC
AACGCTTTTCGGGAGTCAGT

Downstream 100 bases:

>100_bases
TAAAGCATCAGGTTTCCTGCTATCCTTGGCGGGAATTGAATACAGGAGAGAGTTATGTATCCCGTCGACCTTCATATGCA
TACCGTTGCCAGCACGCATG

Product: 2-ketoacid reductase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDIIFYHPTFDTQWWIEVLRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPATVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITCPAEAVDYISRTIAQLEKGERVCGQVDRARGY

Sequences:

>Translated_312_residues
MDIIFYHPTFDTQWWIEVLRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPATVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITCPAEAVDYISRTIAQLEKGERVCGQVDRARGY
>Mature_312_residues
MDIIFYHPTFDTQWWIEVLRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPATVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITCPAEAVDYISRTIAQLEKGERVCGQVDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=29.1139240506329, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=26.8199233716475, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=98.3974358974359, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=27.4436090225564, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=25.9433962264151, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574286, Length=262, Percent_Identity=29.3893129770992, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45552429, Length=238, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585514, Length=238, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574282, Length=238, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574284, Length=238, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45551003, Length=238, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921140, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35261; Mature: 35261

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIFYHPTFDTQWWIEVLRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPATVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITCPAEAVDYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHH
RVCGQVDRARGY
HHHHHHHHHCCC
>Mature Secondary Structure
MDIIFYHPTFDTQWWIEVLRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPATVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITCPAEAVDYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHH
RVCGQVDRARGY
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA