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The map label for this gene is yebU

Identifier: 218699594

GI number: 218699594

Start: 1282079

End: 1283518

Strand: Reverse

Name: yebU

Synonym: ECIAI39_1215

Alternate gene names: 218699594

Gene position: 1283518-1282079 (Counterclockwise)

Preceding gene: 218699595

Following gene: 218699593

Centisome position: 25.01

GC content: 52.64

Gene sequence:

>1440_bases
GTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCAATGCCTTCGACGCTCTCATTTGA
TGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGTATTCGCGTTAATACGCTGAAAATCTCCGTTGCTGATTTCC
TGCAATTAACCGCCCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAAT
GAAGATGCATTGCCGTTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAGCCAGTTCAATGTTGCC
CGTCGCCGCCTTGTTTGCTGACGGTAATGCACCACAGCGGGTGATGGATGTCGCTGCCGCGCCCGGCTCCAAAACGACGC
AAATTGCCGCGCGGATGAATAACGAAGGGGCGATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCC
AATATCAGCCGCTGTGGCATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCATTGCCAGAAAT
GTTCGATGCCATTTTGCTGGACGCTCCCTGCTCCGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGT
CACCAGAAAGCAATCAGGAAATCGCAGCGACCCAACGGGAACTGATCGACAGCGCCTTTCATGCATTACGCCCTGGCGGT
ACGCTGGTTTACTCGACCTGTACCTTAAACAGGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGA
CGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGCAAATAAGGCGCTGACCGAAGAAGGCTTTTTGCATGTTT
TCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATCCCCGTCTTACCCGCCCCA
AAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAGGTGT
TGGCTTAAACTGGGATGGAAACCTGCGACTCTGGCAACGCGACAAAGAACTGTGGTTGTTCCCGGTAGGCATTGAAGCCC
TGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAAACGCATAACAAAGGTTATCGCTGGCAGCATGAA
GCGGTTATTGCCCTTGCATCCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGGAAGCGGAAGAGTGGTATCGCGG
GCGCGATGTTTACCCGCAAGCCGCGCCAGTAGCGGATGATGTATTGGTTACTTTCCAGCATCAGCCGATTGGTTTAGCCA
AACGAATTGGTTCGCGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGAGACGGGAAACTTTTTACCGGTAACGCCTGA

Upstream 100 bases:

>100_bases
GGAACAGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTT
TTTGCCAGTGGTACATGCTC

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAACCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDN
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAP
KYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Sequences:

>Translated_479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDN
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAP
KYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
>Mature_478_residues
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNE
DALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHAN
ISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGGT
LVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPK
YKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEA
VIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=306, Percent_Identity=36.9281045751634, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI76150623, Length=306, Percent_Identity=36.9281045751634, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=38.3233532934132, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI32698918, Length=160, Percent_Identity=35.625, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI39995082, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI23199998, Length=356, Percent_Identity=28.3707865168539, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI270288816, Length=239, Percent_Identity=31.3807531380753, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI270288818, Length=321, Percent_Identity=28.9719626168224, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI8922322, Length=228, Percent_Identity=31.140350877193, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=30, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=98.5386221294363, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=220, Percent_Identity=32.2727272727273, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=33.4375, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=193, Percent_Identity=32.1243523316062, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=26.7080745341615, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=28.82096069869, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=36.8243243243243, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=32.0388349514563, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI22024126, Length=334, Percent_Identity=34.7305389221557, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=33.6538461538462, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=35.4285714285714, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53148; Mature: 53017

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQR
CCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHCEEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQR
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTPQE
CCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure 
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQR
CCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHCEEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQR
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTPQE
CCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA