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The map label for this gene is hcr

Identifier: 218699248

GI number: 218699248

Start: 899218

End: 900186

Strand: Reverse

Name: hcr

Synonym: ECIAI39_0853

Alternate gene names: 218699248

Gene position: 900186-899218 (Counterclockwise)

Preceding gene: 218699249

Following gene: 218699247

Centisome position: 17.54

GC content: 55.52

Gene sequence:

>969_bases
ATGACGATGCCAACGAATCAATGCCCGTGGCGGATGCAGGTTCATCACATTACGCAAGAAACGCCGGATGTGTGGACGAT
TTCCCTGATTTGCCACGATTACTACCCATATCGCGCCGGGCAATATGCACTGGTCAGCGTGCGTAACTCAGCGGAAACGC
TGCGTGCTTACACCATTTCCTCCACGCCAGGCGTGAGTGAATACATCACCCTGACCGTGCGGCGGATTGATGACGGTGTC
GGCTCCCGGTGGCTGACGCGCGATGTAAAACGCGGTGATTATCTCTGGCTTTCAGACGCAATGGGGGAATTTACCTGCGA
CGATAAAGCAGAAGATAAATTCCTGTTGCTGGCGGCAGGCTGCGGCGTCACGCCGATTATGTCGATGCGTCGCTGGCTGG
CGAAGAACCGTCCGCAGGCCGATGTGCAGGTGATCTATAACGTGCGTTCGCCGCAGGATGTTATTTTCGCCGATGAGTGG
CGTAACTATCCGGTAACGCTGGTGGCGGAAAATAACGTTACCGAAGGCTTTATCGCTGGTCGTCTCACTCGCGAACTGCT
GGCAGGTGTCCCTGATTTAGCCTCACGTACCGTGATGACCTGTGGCCCTGCTCCATATATGGATTGGGTAGAGCAGGAAG
TGAAAGCGCTCGGCGTGACGCGTTTCTTTAAAGAGAAATTCTTCACCCCAGTAGCGGAAGCGGCGATCAGCGGTCTGAAA
TTCACCAAACTGCAACCGGCACGAGAATTTTACGCCCCGGTTGGCACCACGCTACTGGAGGCGCTGGAAAGCAATAACGT
TCCGGTTGTCGCCGCCTGCCGCGCAGGTGTTTGCGGCTGCTGTAAGACGAAAGTGGTTTCCGGTGAATATACGGTGAGCA
GCACAATGACGCTGACCGACGCCGAAATCGCTGAAGGTTACGTACTGGCCTGCTCCTGCCATCCGCAGGGGGATTTGGTT
CTCGCATAA

Upstream 100 bases:

>100_bases
CAGACCTGCTGGCGGTGCTGAACGAGAAATTCGGCCTGCGTTCTATCACCACTGTTGAAGAAGACATGAAGCAACTGTTG
AGCGCGTAAGGAGTTTGTCG

Downstream 100 bases:

>100_bases
TCGCCTTATGCCCGATGATATTCCTTTCATCGGGCTATTTAACCGTTAGTGCCTCCTTTTTCTCCCATCCCTTCCCCCTC
CGTCAGATGAACTAAACTTG

Product: HCP oxidoreductase, NADH-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAAISGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA

Sequences:

>Translated_322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAAISGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA
>Mature_321_residues
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVG
SRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEWR
NYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAAISGLKF
TKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVL
A

Specific function: NADH oxidoreductase acting in concert with HCP [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=322, Percent_Identity=98.7577639751553, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=338, Percent_Identity=28.1065088757396, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788104, Length=289, Percent_Identity=28.3737024221453, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 35738; Mature: 35607

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAAISGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC
>Mature Secondary Structure 
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAAISGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10651802 [H]