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The map label for this gene is poxB

Identifier: 218699247

GI number: 218699247

Start: 897367

End: 899085

Strand: Reverse

Name: poxB

Synonym: ECIAI39_0852

Alternate gene names: 218699247

Gene position: 899085-897367 (Counterclockwise)

Preceding gene: 218699248

Following gene: 218699246

Centisome position: 17.52

GC content: 53.52

Gene sequence:

>1719_bases
ATGAAACAAACGGTTGCAGCTTATATCGCCAAAACACTCGAATCGGCAGGAGTGAAACGCATCTGGGGAGTCACGGGCGA
CTCTCTGAACGGTCTTAGTGACAGTCTTAATCGCATGGGCACCATCGAGTGGATGTCCACCCGGCATGAAGAAGTGGCGG
CGTTTGCCGCTGGCGCTGAAGCACAACTTAGCGGAGAACTGGCGGTCTGTGCCGGATCGTGCGGCCCCGGCAACCTGCAC
TTAATCAACGGCCTGTTCGATTGCCACCGCAATCACGTTCCGGTACTGGCGATTGCCGCTCATATTCCCTCCAGCGAAAT
TGGCAGCGGCTATTTCCAGGAAACCCACCCACAAGAGCTATTCCGCGAATGTAGTCACTATTGCGAGCTGGTTTCCAGCC
CAGAGCAGATCCCACAAGTGCTGGCAATTGCTATGCGCAAAGCAGTGCTAAACCGTGGCGTTTCCGTTGTCGTGTTACCG
GGCGACGTGGCGTTAAAACCTGCACCAGAAGGGGCAACTACCCACTGGTATCATGCGCCACAACCAGTCGTGACGCCGGA
AGAAGAAGAGTTACGCAAACTGGCGCAACTGCTGCGTTATTCCAGCAATATCGCCCTGATGTGTGGCAGCGGCTGCGCGG
GGGCGCATAAAGAGTTAGTTGAGTTTGCCGGGAAAATTAAAGCGCCTATTGTTCATGCCCTGCGCGGTAAAGAGCATGTC
GAATACGATAATCCGTATGATGTCGGAATGACGGGATTAATCGGCTTCTCGTCAGGTTTCCATACCATGATGAATGCCGA
TACGTTAGTGCTGCTCGGCACGCAATTTCCCTACCGCGCGTTCTACCCGACCGATGCCAAAATTATTCAGATTGATATCA
ACCCAGCCAGCATCGGCGCGCATAGCAAGGTAGATATGGCGCTGGTCGGCGATATCAAATCAACCCTGCGGGCATTGCTG
CCACTGGTGGAAGAAAAAACCGATCGCAAGTTTCTGGATAAAGCGCTGGAAGATTACCGCGACGCCCGCAAAGGGCTGGA
TGATTTAGCTAAACCGAGCGAGAAAGCCATTCACCCGCAATATCTGGCGCAGCAAATTAGTCATTTTGCCGCCGATGACG
CCATCTTTACCTGTGACGTTGGTACGCCAACGGTGTGGGCGGCACGTTATCTGAAAATGAACGGCAAGCGTCGTCTGTTA
GGTTCGTTTAACCACGGTTCGATGGCTAACGCCATGCCGCAGGCGCTGGGTGCGCAGGCAACCGAGCCGGAACGTCAGGT
GGTCGCCATGTGCGGCGATGGCGGTTTCAGTATGTTGATGGGCGATTTCCTCTCAGTAATGCAGATGAAATTGCCAGTGA
AAATTATCGTCTTTAATAACAGCGTGCTGGGCTTTGTGGCGATGGAGATGAAAGCCGGAGGCTACCTGACAGACGGTACT
GAGCTGCACGACACCAACTTTGCCCGCATTGCCGAAGCGTGCGGAATTACGGGTATTCGCGTGGAAAAAGCCTCTGAAGT
CGATGAAGCCCTGCAACGCGCCTTTTCCATCGACGGTCCGGTGTTGGTGGATGTGGTAGTAGCCAAAGAAGAGTTAGCCA
TTCCACCGCAGATCAAACTTGAACAGGCCAAAGGATTTAGCTTGTATATGTTGCGCGCAATCATCAGCGGACGCGGTGAT
GAAGTGATCGAACTGGCGAAAACGAACTGGCTAAGGTAA

Upstream 100 bases:

>100_bases
GGCTATTTAACCGTTAGTGCCTCCTTTTTCTCCCATCCCTTCCCCCTCCGTCAGATGAACTAAACTTGTTACCGTTATCA
CATTCAGGAGATGGAGAACC

Downstream 100 bases:

>100_bases
AAAGGGTGGCATTTCCCGTCATAATAAGGACATGCCATGATTGATTTACGCAGTGATACCGTTACCCGACCGAGCCGCGC
CATGCTCGAAGCAATGATGG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVMQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Sequences:

>Translated_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVMQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR
>Mature_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVMQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=23.7924865831843, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=99.3006993006993, Blast_Score=1178, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=30.1785714285714, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=28.6751361161525, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=24.8175182481752, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=22.962962962963, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=27.0796460176991, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=26.2969588550984, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=25.2688172043011, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=23.0627306273063, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922626, Length=553, Percent_Identity=24.2314647377938, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62058; Mature: 62058

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCEECC
QPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVMQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCEECC
QPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVMQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]