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The map label for this gene is ycjZ [C]

Identifier: 218698798

GI number: 218698798

Start: 428742

End: 429716

Strand: Reverse

Name: ycjZ [C]

Synonym: ECIAI39_0390

Alternate gene names: 218698798

Gene position: 429716-428742 (Counterclockwise)

Preceding gene: 218698799

Following gene: 218698794

Centisome position: 8.37

GC content: 50.05

Gene sequence:

>975_bases
GTGGTTAAAATAATAGCATCTATGCCCCTCTTCATGAGTGTCTGTGAAGAGGATGCTGATGCTTCTACAAGGAAATCGCC
AATGCTCAAAGAAAACTTCAACGAACTGCAAATCTTTCTTGTGGTGGCAAGAGAGCGAAGTTTTACCAAAGCAGCGGGCA
AACTCGGCGTCTCTCAGTCTGCACTTAGCCATGCAATGAAGGCTCTGGAGGAAAGGCTCAATATCCGCCTCCTGACACGG
ACTACCCGCAGTGTGGCTCCCACAGAAGCGGGGGAGAGAATCATTGCCTGCCTTGAACCGCGTATTGACGAGCTTGAACA
GGAACTGGAATCACTTATTCAATTGAACGGCACGCCCTCCGGGAATATTCGGTTATCTGCCGGGGAACATGCCGCGCGAA
GTCTGGTCTGGCCGAAGCTAAAACCTTTTCTCAGAGAGTATCCCGAAATCAATGTCGAACTGGTGGTGGATAACGGTTTT
GTCGATATTGTTGAAGGCCGTTTTGATGCCGGGATACGACTGGGTGAAAATGTTGATAAAGATATGGTTGCAGTCAGGAT
TGGACCAGATATGCGGATGGCTGTGGTGGGAGCTCCGGCGTATTTTGCTGCAAACCCTGCGCCTGAAACGCCTCATGAGC
TACAAAATCATCGCTGCATCAACATGCGTTTGCCAACTGCCGGTGGGCTTTACCACTGGGAGTTCGAGCGAGAAGGGAAA
CCGCTTCGCGTTAAGGTTGATGGGCAACTGACGTGTAGTCCATTGCCAGAAAGAATTGATGCCGCGTTATCAGGGTTTGG
GATCGCTTGTGTCCCTGAAGACATGGTTCAGGAGTATATAAAGTCAGGCAAGCTTATTCAGGTGTTACAGGAGTGGTGTC
CAACTTTTCCTGGGTATTATCTTTACTACCCCAGCCGCAAGCAGCATCCGCCAGCTTTTGCGCTGTTGATCGATGCGCTT
CGCTACACCGAATAA

Upstream 100 bases:

>100_bases
GAAGAATGGCGCAATCGTGAGGATACGTTTATTCATAATAAATCTCCTGAAAGTTTAGAATTATTTTCGATACAGCCAGA
TTATCATTGTTACTAATAAT

Downstream 100 bases:

>100_bases
CGGGTCCTTTATCCTGGACCCGTTTGGGTATTAGCGGCCTACGCGTGCCTGATGTTCAGGCGAGTAACGTTCACCGACGA
TTTTAATTGTTTCCAGCGCC

Product: putative transcriptional regulator, lysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MVKIIASMPLFMSVCEEDADASTRKSPMLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEERLNIRLLTR
TTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGF
VDIVEGRFDAGIRLGENVDKDMVAVRIGPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGK
PLRVKVDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDAL
RYTE

Sequences:

>Translated_324_residues
MVKIIASMPLFMSVCEEDADASTRKSPMLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEERLNIRLLTR
TTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGF
VDIVEGRFDAGIRLGENVDKDMVAVRIGPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGK
PLRVKVDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDAL
RYTE
>Mature_324_residues
MVKIIASMPLFMSVCEEDADASTRKSPMLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEERLNIRLLTR
TTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGF
VDIVEGRFDAGIRLGENVDKDMVAVRIGPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGK
PLRVKVDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDAL
RYTE

Specific function: May have a role in the regulation of oprD expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=47.4576271186441, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=39.2491467576792, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1786401, Length=280, Percent_Identity=30, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789440, Length=273, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=30, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693193, Length=281, Percent_Identity=29.8932384341637, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=27.9863481228669, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=25.8620689655172, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=25.390625, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693105, Length=150, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=145, Percent_Identity=30.3448275862069, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=131, Percent_Identity=33.587786259542, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790011, Length=275, Percent_Identity=23.2727272727273, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787601, Length=124, Percent_Identity=29.8387096774194, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789283, Length=183, Percent_Identity=31.6939890710383, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36237; Mature: 36237

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIIASMPLFMSVCEEDADASTRKSPMLKENFNELQIFLVVARERSFTKAAGKLGVSQS
CEEEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEEEEEECCCCHHHHHHHCCCCHH
ALSHAMKALEERLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPS
HHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDK
CCEEEECCCHHHHHHCCHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCC
DMVAVRIGPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGK
CEEEEEECCCCEEEEEECCHHEECCCCCCCHHHHHCCEEEEEECCCCCCEEEEEEECCCC
PLRVKVDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYY
CEEEEECCEEECCCCHHHHHHHHHCCCEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCEE
LYYPSRKQHPPAFALLIDALRYTE
EECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVKIIASMPLFMSVCEEDADASTRKSPMLKENFNELQIFLVVARERSFTKAAGKLGVSQS
CEEEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEEEEEECCCCHHHHHHHCCCCHH
ALSHAMKALEERLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPS
HHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDK
CCEEEECCCHHHHHHCCHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCC
DMVAVRIGPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGK
CEEEEEECCCCEEEEEECCHHEECCCCCCCHHHHHCCEEEEEECCCCCCEEEEEEECCCC
PLRVKVDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYY
CEEEEECCEEECCCCHHHHHHHHHCCCEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCEE
LYYPSRKQHPPAFALLIDALRYTE
EECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]