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The map label for this gene is ycaN [H]

Identifier: 218698799

GI number: 218698799

Start: 429841

End: 430860

Strand: Reverse

Name: ycaN [H]

Synonym: ECIAI39_0391

Alternate gene names: 218698799

Gene position: 430860-429841 (Counterclockwise)

Preceding gene: 218698811

Following gene: 218698798

Centisome position: 8.4

GC content: 42.65

Gene sequence:

>1020_bases
TTGTGTTCATATCGAGAACGGCGTAATAGTATGACTTGTATTGGTTATCATCAATGGTCTAATATCTATCACAGTGTTAA
GTTAAGGTTTACAATGATGAAAATAGAGCCTTCAATTTTGCCTTCTCTTGCCTGGTTTGCGCTGATTGTTCGTGCCGGCA
GTTTTTCCAGAGCCGCTTCTGAAATGGGTATTACGCGGGCCGCATTGTCCCAGAATTTAAAATCTCTTGAGGAACGTCTT
AACACCAAGCTGATATACCGTACGACCCGCAATATGTCGCTTACTGAGGAAGGACAGCGTCTGTATGAAGTGCTGGTGTC
GGCTTTGGGGCAAATTGATGATGCGTTGAAAGATGTCGGTGATACCCAACTTGAACCTACAGGACTGTTGAGAATAAATT
CCTCCCGTGTTGCAGCAAGGATGCTTGTTGAGCCTCATATTGGCGAGTTTCTTACTCGTTACCCCAAAACAAAGATAGAA
CTCATTATGGATGACGGTTTATCTAACATTATTGCGGACGGCTGTGATGTGGGCATACGTCTTGAGCAAGGACTGGATGA
GCATATGACTGCCGTTCCTGTTTCTCCGCTTATTAAACTGGTTACTGTGGCATCGCCAGATTATTTAAAAGAACACGGTA
TTCCTGAAACTCCTCAGGAATTGAGTAACCATAATTGTCTTCGGTTGCGGCATAAAAGTAGCGGTGCACTCTCAGCATGG
GAGTTTTCGAATGTTGTGGGGGGTAATGAAGAGTTCGAAATAGAGGTGTCAGGTAAGTATATTTCTAATGATGACGAAAG
CATGATACGAATGGCGCTGAATGGAACGGGTATCATTCAACATCTGGATTTTGCAATCGCTGAACATATCAATGCGGGCA
AGTTACAGCCGATTCTTGAGGATTGGGCCGTTTCATTTCCTGGATTTTATATATATGTTTCATCACGGGTGAGAATGCCC
TCTAAAGTTCGTGCTTTTATTGATTTTATGGTGGAGAAAAGAGTAAAAATAGAAAGTTAA

Upstream 100 bases:

>100_bases
TATCCCAGTAAGTGTGTTTTATCGAAACAGTTTTCATTTCAAGCTCCAGGGATTTGCCTAAACAGATTTAAATATCATTT
CTATTATCCGCAACTGGCAT

Downstream 100 bases:

>100_bases
TGATGTGTGTCAGAATATCGGTAAGAAGAATGGCGCAATCGTGAGGATACGTTTATTCATAATAAATCTCCTGAAAGTTT
AGAATTATTTTCGATACAGC

Product: putative LysR-like transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MCSYRERRNSMTCIGYHQWSNIYHSVKLRFTMMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKSLEERL
NTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIE
LIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAW
EFSNVVGGNEEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMP
SKVRAFIDFMVEKRVKIES

Sequences:

>Translated_339_residues
MCSYRERRNSMTCIGYHQWSNIYHSVKLRFTMMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKSLEERL
NTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIE
LIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAW
EFSNVVGGNEEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMP
SKVRAFIDFMVEKRVKIES
>Mature_339_residues
MCSYRERRNSMTCIGYHQWSNIYHSVKLRFTMMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKSLEERL
NTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIE
LIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAW
EFSNVVGGNEEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMP
SKVRAFIDFMVEKRVKIES

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=38.1443298969072, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=30.4498269896194, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=34.2465753424658, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1789440, Length=282, Percent_Identity=30.8510638297872, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=28.7671232876712, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=26.510067114094, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=28.0821917808219, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=25.2525252525253, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790011, Length=302, Percent_Identity=24.8344370860927, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693105, Length=159, Percent_Identity=27.0440251572327, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=27.4509803921569, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=21.5547703180212, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38182; Mature: 38182

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSYRERRNSMTCIGYHQWSNIYHSVKLRFTMMKIEPSILPSLAWFALIVRAGSFSRAAS
CCCCHHHCCCCEEEEEHHHHHHHHEEEEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHH
EMGITRAALSQNLKSLEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVG
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIR
CCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCEE
LEQGLDEHMTAVPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAW
HHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCHH
EFSNVVGGNEEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILE
HHHHHCCCCCEEEEEECCCEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHH
DWAVSFPGFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCSYRERRNSMTCIGYHQWSNIYHSVKLRFTMMKIEPSILPSLAWFALIVRAGSFSRAAS
CCCCHHHCCCCEEEEEHHHHHHHHEEEEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHH
EMGITRAALSQNLKSLEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVG
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIR
CCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCEE
LEQGLDEHMTAVPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAW
HHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCHH
EFSNVVGGNEEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILE
HHHHHCCCCCEEEEEECCCEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHH
DWAVSFPGFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]