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The map label for this gene is aroP

Identifier: 218698532

GI number: 218698532

Start: 130282

End: 131655

Strand: Reverse

Name: aroP

Synonym: ECIAI39_0112

Alternate gene names: 218698532

Gene position: 131655-130282 (Counterclockwise)

Preceding gene: 218698537

Following gene: 218698529

Centisome position: 2.57

GC content: 52.91

Gene sequence:

>1374_bases
ATGATGGAAGGTCAACAGCACGGCGAGCAGCTAAAGCGCGGCCTTAAAAACCGCCATATTCAGCTTATCGCGCTGGGTGG
CGCGATAGGGACCGGGCTATTCCTGGGTAGCGCCTCCGTAATACAGTCTGCAGGGCCAGGGATTATCCTGGGTTACGCCA
TTGCTGGTTTTATCGCCTTTCTGATCATGCGTCAGCTAGGTGAAATGGTGGTCGAAGAACCTGTCGCAGGCTCCTTTAGC
CACTTTGCTTATAAATACTGGGGCAGTTTTGCCGGTTTCGCCTCTGGCTGGAACTACTGGGTACTGTACGTTTTAGTTGC
CATGGCAGAGCTGACTGCCGTGGGTAAATACATTCAGTTCTGGTATCCGGAAATCCCAACCTGGGTTTCTGCCGCCGTGT
TCTTTGTGGTGATCAACGCCATCAACCTGACAAACGTAAAAGTGTTTGGCGAGATGGAGTTCTGGTTTGCCATTATCAAA
GTTATCGCGGTGGTAGCGATGATCATCTTCGGCGGCTGGCTGCTATTCAGTGGCAACGGCGGCCCGCAGGCGACCGTTAG
CAACCTGTGGGATCAGGGTGGTTTCCTGCCGCACGGCTTCACCGGGCTGGTGATGATGATGGCGATTATCATGTTCTCGT
TCGGTGGTCTGGAACTGGTGGGGATCACCGCAGCAGAAGCTGATAACCCGGAGCAAAGTATTCCAAAAGCGACTAACCAG
GTTATCTACCGCATCCTGATTTTCTACATTGGTTCGTTAGCCGTTCTGCTCTCACTGATGCCGTGGACACGCGTTACCGC
TGACACCAGCCCGTTTGTGCTGATCTTCCACGAGTTAGGCGATACCTTTGTGGCAAATGCGCTGAACATCGTGGTACTGA
CTGCGGCGCTCTCCGTGTACAACAGCTGCGTATATTGCAACAGCCGTATGCTGTTTGGTCTGGCACAACAGGGCAACGCG
CCAAAAGCGCTGGCGTCTGTCGACAAACGCGGTGTACCGGTTAATACTATTCTGGTGTCTGCGCTGGTTACGGCATTGTG
CGTGCTGATTAACTACCTTGCACCGGAATCCGCATTCAGCCTGTTAATGGCGCTGGTGGTATCTGCACTGGTCATCAACT
GGGCGATGATTAGCCTGGCGCATATGAAATTCCGTCGCGCCAAGCAGGAACAAGGCGTGGTAACTCGCTTCCCTGCTCTG
CTTTATCCGCTGGGTAACTGGATCTGCCTGCTGTTTATGGCGGCGGTATTGGTGATTATGCTGATGACCCCAGGAATGGC
GATTTCGGTATACCTGATCCCGGTATGGCTGATCGTGTTAGGTATCGGCTATCTGTTTAAAGAGAAAACCGCCAAAGCCG
TAAAAGCGCATTAA

Upstream 100 bases:

>100_bases
AAACTTCTTTGATGTAAACAAATTAATACAACAAACGGAATTGCAAACTTACACACGCATCACTGCGTAGATCAAAAAAA
CAATCACCGCACGAGGTTTC

Downstream 100 bases:

>100_bases
TCTCTCTACGCCCTCACCCGTGCAGGGTGAGGGCAATAATCTTTACACCAACGCCCCGTAAATCGTCAGTAGCGCAATTA
CCACCACGACCACGAACGAG

Product: aromatic amino acid transporter

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm]; Proton [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: General aromatic amino acid permease [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFS
HFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNA
PKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFSLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPAL
LYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH

Sequences:

>Translated_457_residues
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFS
HFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNA
PKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFSLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPAL
LYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
>Mature_457_residues
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFS
HFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNA
PKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFSLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPAL
LYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of the aromatic amino acids (phenylalanine, tyrosine, and tryptophan) [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI258645169, Length=378, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI110347453, Length=356, Percent_Identity=23.876404494382, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI258614005, Length=379, Percent_Identity=23.2189973614776, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI258614003, Length=379, Percent_Identity=23.2189973614776, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI181337167, Length=292, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4507047, Length=389, Percent_Identity=21.5938303341902, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786302, Length=457, Percent_Identity=99.781181619256, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=430, Percent_Identity=64.4186046511628, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1790653, Length=461, Percent_Identity=40.3470715835141, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1786602, Length=453, Percent_Identity=43.046357615894, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI48994972, Length=431, Percent_Identity=40.1392111368909, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI87081915, Length=456, Percent_Identity=36.8421052631579, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87081708, Length=465, Percent_Identity=35.0537634408602, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1789017, Length=433, Percent_Identity=36.7205542725173, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788480, Length=408, Percent_Identity=35.5392156862745, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87081869, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531343, Length=380, Percent_Identity=25.7894736842105, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532491, Length=398, Percent_Identity=26.6331658291457, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533459, Length=372, Percent_Identity=23.3870967741935, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=449, Percent_Identity=35.8574610244989, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6324061, Length=440, Percent_Identity=33.6363636363636, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324924, Length=458, Percent_Identity=33.4061135371179, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324553, Length=398, Percent_Identity=33.9195979899498, Blast_Score=216, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6320717, Length=406, Percent_Identity=32.512315270936, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6321053, Length=422, Percent_Identity=31.5165876777251, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322892, Length=431, Percent_Identity=34.5707656612529, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6319824, Length=402, Percent_Identity=31.592039800995, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=30.4250559284116, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6324059, Length=440, Percent_Identity=33.1818181818182, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6321629, Length=400, Percent_Identity=30.5, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319543, Length=398, Percent_Identity=30.9045226130653, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320251, Length=405, Percent_Identity=29.6296296296296, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319542, Length=406, Percent_Identity=28.8177339901478, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319608, Length=418, Percent_Identity=28.9473684210526, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6322967, Length=399, Percent_Identity=30.0751879699248, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324981, Length=417, Percent_Identity=28.7769784172662, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320364, Length=472, Percent_Identity=26.4830508474576, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24667468, Length=391, Percent_Identity=25.3196930946292, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666159, Length=370, Percent_Identity=21.8918918918919, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221512776, Length=375, Percent_Identity=21.8666666666667, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 49721; Mature: 49721

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAF
CCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHH
LIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
WYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNG
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
GPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHH
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVY
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHH
NSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFS
HHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAVLVIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAF
CCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHH
LIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
WYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNG
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
GPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHH
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVY
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHH
NSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFS
HHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAVLVIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm]; Proton [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + L-tyrosine [Periplasm] = Proton [Cytoplasm] + L-tyrosine [Cytoplasm] Proton [Periplasm] + L-phenylalanine [Periplasm] = Proton [Cytoplasm] +

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]