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The map label for this gene is srlE

Identifier: 218696294

GI number: 218696294

Start: 3037396

End: 3038355

Strand: Direct

Name: srlE

Synonym: EC55989_2965

Alternate gene names: 218696294

Gene position: 3037396-3038355 (Clockwise)

Preceding gene: 218696293

Following gene: 218696295

Centisome position: 58.92

GC content: 54.79

Gene sequence:

>960_bases
ATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT
CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGAT
TTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTAT
CCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATAT
TTATGTCTCTGGCGTAAAAGAAGAAAATATCACTGTAGTGGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT
ATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCGCCGTTGCG
GTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCT
CATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTC
TGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATC
GGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGC
GCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTT
CTGTACTGGTGAGCCGCTTTTTAACCGGCGCACCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAG

Upstream 100 bases:

>100_bases
ATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTT
CACCTGGCAGGAGCAACATC

Downstream 100 bases:

>100_bases
AGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCACTGACGCCCTCAGCGACCAGA
TGCTCATCACCTTTCGTGAA

Product: glucitol/sorbitol-specific enzyme IIB component of PTS

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIY
PKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVI
GVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ

Sequences:

>Translated_319_residues
MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIY
PKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVI
GVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ
>Mature_318_residues
THIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYP
KRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAV
LFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVIG
VLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=319, Percent_Identity=99.6865203761756, Blast_Score=634, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 33302; Mature: 33171

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPA
CCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCC
EAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVV
CCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
GDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTVLKTILPF
ECCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
MAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRF
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHCCHHHHHHHHH
LTGAPTVLIAWFVSGFIYQ
HCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPA
CEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCC
EAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVV
CCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
GDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTVLKTILPF
ECCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
MAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRF
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHCCHHHHHHHHH
LTGAPTVLIAWFVSGFIYQ
HCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503 [H]