Click here to switch to the map view.

The map label for this gene is srlA

Identifier: 218696293

GI number: 218696293

Start: 3036836

End: 3037399

Strand: Direct

Name: srlA

Synonym: EC55989_2964

Alternate gene names: 218696293

Gene position: 3036836-3037399 (Clockwise)

Preceding gene: 218696282

Following gene: 218696294

Centisome position: 58.91

GC content: 50.0

Gene sequence:

>564_bases
ATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGT
GACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTG
AACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGC
AATCCGATGACCTTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTG
CCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAA
CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTG
ACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATC
ATGA

Upstream 100 bases:

>100_bases
TCTTTCATTTTGAGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAAT
AAATCCTGAAGGAGAGAACA

Downstream 100 bases:

>100_bases
CGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTC
TATATCACCGCCGGTACCCG

Product: glucitol/sorbitol-specific enzyme IIC component of PTS

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Gut; PTS system glucitol/sorbitol-specific EIIC component

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLAGATS

Sequences:

>Translated_187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLAGATS
>Mature_187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLAGATS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3730

COG function: function code G; Phosphotransferase system sorbitol-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-5 domain

Homologues:

Organism=Escherichia coli, GI48994904, Length=187, Percent_Identity=100, Blast_Score=382, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTHC_ECOLI (P56579)

Other databases:

- EMBL:   J02708
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X70016
- PIR:   A26725
- RefSeq:   AP_003269.1
- RefSeq:   YP_026180.1
- ProteinModelPortal:   P56579
- STRING:   P56579
- EnsemblBacteria:   EBESCT00000004056
- EnsemblBacteria:   EBESCT00000017197
- GeneID:   947575
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5429
- KEGG:   eco:b2702
- EchoBASE:   EB0962
- EcoGene:   EG10969
- eggNOG:   COG3730
- GeneTree:   EBGT00050000010466
- HOGENOM:   HBG297191
- OMA:   EYYKPSY
- ProtClustDB:   CLSK863821
- BioCyc:   EcoCyc:G8210-MONOMER
- Genevestigator:   P56579
- InterPro:   IPR004699
- TIGRFAMs:   TIGR00821

Pfam domain/function: PF03608 EII-GUT

EC number: 2.7.1.69

Molecular weight: Translated: 20581; Mature: 20581

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS51107 PTS_EIIC_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b8fc85c)-; HASH(0x1a52a108)-; HASH(0x1b75e53c)-;

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQHRIERFAQRC
CCCCHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHCCCCEEEECCCCHHCCCCCCCCCCCC
ELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWVTDLTTAIFEKKMGIQLEQKV
CEEEEEEHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHE
HLAGATS
ECCCCCH
>Mature Secondary Structure
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQHRIERFAQRC
CCCCHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHCCCCEEEECCCCHHCCCCCCCCCCCC
ELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWVTDLTTAIFEKKMGIQLEQKV
CEEEEEEHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHE
HLAGATS
ECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503; 1334233