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The map label for this gene is wcaM

Identifier: 218695666

GI number: 218695666

Start: 2344642

End: 2346036

Strand: Reverse

Name: wcaM

Synonym: EC55989_2299

Alternate gene names: 218695666

Gene position: 2346036-2344642 (Counterclockwise)

Preceding gene: 218695667

Following gene: 218695665

Centisome position: 45.51

GC content: 47.67

Gene sequence:

>1395_bases
ATGCCATTTAAAACACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCCCTTTCGCTCG
CGCACTTCCCGCCCGACAAAGCGTTAACATTAACGACTACAACCCACACGACTGGATCGCCTCATTTAAACAAGCCTTCG
GCGAAGGGCAAACGGTCGTCGTGCCTGCCGGATTGGTTTGCGACAATATCAACACCGGCATCTTCATTCCTCCTGGCAAA
ACGTTACACATTCTTGGAAGCCTGCGCGGCAGCGGCAGAGGGCGATTTGTCTTACAGGACGGCAGCCAGGTGACAGGGGA
GAAGGGCGGCAGTATGCATAACATCACCCTGGATGTGCGCGGCTCTGACTGCACTATTAAAGGGCTGGCGATGAGCGGCT
TTGGCCCGGTAACGCAGATTTATATTGGCGGCAAAAACAAACGAGTCATGCGTAACCTGACCATCGATAACCTGACCGTT
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATTATCGGTGGCAACATCACCAACTGTAAGTTCAG
CGACTTACAGGGGGACGCTATTGAATGGAACGTGGCGATTAACGACCGTGATATTTTGATATCTGACCATGTCATCGAGC
GCATCAACTGTACTAACGGCAAAATCAACTGGGGCATCGGCATAGGCCTTGCGGGGAGTACTTATGATAATAACTACCCG
GAAGACCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATCCATGTTGAAAATGG
TAAACATTTTGTTATTCGTAATATCAAAGCCCGCAATATCACGCCGGATTTCAGTAAGAAAGCAGGTATTGATAACGCGA
CAGTCGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGATTAATAGCGCCGGGATGTTAATCGGCTAT
GGGGTAATTAAAGGCAAATATCTCTCGATACCGCAAAATTTCCGAGTGAATAATATTCAACTGGATAATACCCACCTTGC
TTACAAATTGCGCGGTATCCAAATCTCTGCCGGGAATGCTGTCTCCTTTGTGGCGCTGACTAACATTGAGATGAAGCGTG
CGTCGCTTGAGCTACACAATAAACCGCAACACCTTTTTATGCGTAATATCAATGTGATGCAGGAATCCTCAGTTGGACCA
GCATTGAGTATGAACTTCGACATGCGCAAAGACGTTCGCGGCGTCTTTATGGCGAAAAAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAAAGAGGGCAAAGCTCCGTCGATATCGACAGGATTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGAGAGTAG

Upstream 100 bases:

>100_bases
GTCGTCAAACGTGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCCAGCTTGCTGCA
AGCTTTATAGAGGAGAACGC

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCATAAACTTACTGGATATAATGTTCTAAAAGATTATAATCCTCACACTAACTGTTAAAGCTGGGGCT
GGGCAGCATTGACTACTCAG

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGSGRGRFVLQDGSQVTGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGGNITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGSGRGRFVLQDGSQVTGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGGNITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPPGKT
LHILGSLRGSGRGRFVLQDGSQVTGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGGNITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
VIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPA
LSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=97.6293103448276, Blast_Score=933, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51225; Mature: 51094

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFGEGQTVV
CCCCCCCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGSGRGRFVLQDGSQVTGEKGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGGN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCCEEHHHCCCEEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFGEGQTVV
CCCCCCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGSGRGRFVLQDGSQVTGEKGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGGN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCCEEHHHCCCEEECCCCCCEEEECCCHHHCCHHEEEEHH
ETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]