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The map label for this gene is wcaL

Identifier: 218695667

GI number: 218695667

Start: 2346047

End: 2347267

Strand: Reverse

Name: wcaL

Synonym: EC55989_2300

Alternate gene names: 218695667

Gene position: 2347267-2346047 (Counterclockwise)

Preceding gene: 218695668

Following gene: 218695666

Centisome position: 45.54

GC content: 54.63

Gene sequence:

>1221_bases
ATGAAGGTCGGCTTCTTTTTACTGAAATTTCCGCTGTCTTCGGAAACCTTCGTCCTCAATCAAATTACCGCGTTTATTGA
TATGGGCTTTGAGGTGGAGATTGTCGCGCTGCAAAAAGGCGACACCCAGAACACCCACGCGGCATGGACGAAATACAACC
TTGCCGCCAGAACCCGCTGGTTACAGGACGAACCTACGGGCAAAGTGGCGAAGCTACGCCACCGTGCCAGCCAGACGTTA
CGAGGCATTCATCGTAAAAATACCTGGCAGGCGCTCAACCTCAAACGCTATGGCGCAGAGTCGCGGAATCTGATTTTGTC
TGCCATTTGCGGCCAGGTCGCAACACCGTTTCGCGCCGATGTGTTCATCGCTCATTTTGGTCCAGCGGGGGTAACCGCAG
CAAAACTCCGCGAACTGGGTGTCATTCGCGGCAAAATTGCCACCATTTTCCACGGCATTGATATTTCCAGTCGGGAAGTG
CTCAACCACTACACTCCCGAATATCAACAACTGTTTCGCCGTGGCGACCTGATGTTACCGATAAGCGATTTGTGGGCCGG
AAGGCTGCAAAAAATGGGCTGCCCGAGGGAAAAAATCGCCGTATCGCGTATGGGCGTGGACATGACGCGCTTTAGCCCGC
GTCCGGTGAAAGCGCCCGCAACGCCGCTGGAAATCATTTCCGTCGCACGCTTAACCGAGAAAAAAGGCCTGCATGTGGCG
ATCGAAGCCTGCCGTCAGTTGAAAGAGCAGGGCGTGGCATTTCGCTATCGCATCCTCGGCATTGGCCCGTGGGAACGACG
CCTGCGCACCCTCATCGAACAATATCAACTGGAAGATGTGGTGGAGATGCCGGGCTTTAAACCGAGCCACGAAGTGAAAG
CGATGCTCGACAACGCGGATGTCTTCCTGTTGCCATCGGTTACAGGCGCGGATGGCGATATGGAAGGTATTCCGGTGGCA
CTAATGGAAGCAATGGCGGTCGGCATTCCGGTTGTTTCAACTCTGCATAGCGGAATACCAGAACTGGTGGAGGCCGATAA
ATCCGGCTGGCTGGTGCCTGAGAACGATGCTCACGCACTGGCGCAACGACTAGCGGCGTTTAGCCAACTGGACACCGACG
AACTGGCTCCGGTCGTCAAACGTGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCC
AGCTTGCTGCAAGCTTTATAG

Upstream 100 bases:

>100_bases
TTCCGGCGCTGAACGCACGACTTAGCGAAGCCGTCAGTCGTGAGCGTCAGACGGGAATGCAGATGGTGCAATCTGTGCTT
GAGCGCATCGGGGAGGTGAA

Downstream 100 bases:

>100_bases
AGGAGAACGCATGCCATTTAAAACACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCC
CTTTCGCTCGCGCACTTCCC

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDNADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDAHALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL

Sequences:

>Translated_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDNADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDAHALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL
>Mature_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDNADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDAHALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=99.2610837438424, Blast_Score=830, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45328; Mature: 45328

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRW
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
LQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECHHHCCCCHHHHHHHHHHHHHCCCCCCC
VFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDNAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDAHAL
EEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRW
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
LQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECHHHCCCCHHHHHHHHHHHHHCCCCCCC
VFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDNAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDAHAL
EEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]