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The map label for this gene is paaK

Identifier: 218694944

GI number: 218694944

Start: 1579590

End: 1580903

Strand: Direct

Name: paaK

Synonym: EC55989_1534

Alternate gene names: 218694944

Gene position: 1579590-1580903 (Clockwise)

Preceding gene: 218694943

Following gene: 218694945

Centisome position: 30.64

GC content: 52.05

Gene sequence:

>1314_bases
ATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAACGTCTGAA
ATGGACGCTCAAACACGCGTATGAAAATGTCCCGATGTATCGGCGCAAATTTGACGCAGCAGGCGTACATCCTGATGATT
TCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTT
GCCGTGCCGATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGTTATACGCA
AAACGATATTGATAACTGGGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATG
TTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGCGCGCACTATGGTGCCGAACGTCTGGGCGCTACGGTGATCCCG
ATGTCTGGCGGGCAGACGGAAAAACAAGCGCAACTGATCCGCGATTTTCAACCAGATATGATCATGGTTACGCCATCTTA
TTGCCTTAACCTGATTGAAGAGCTGGAGCGGCAGTTGGGCGGTGATGCCAGCGGTTGCTCGCTGCGGGTTGGCGTGTTTG
GTGCTGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAACGGCGTCTGGGGATCACCGCACTGGATATTTATGGCCTG
TCAGAAGTGATGGGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGTCCAACTATTTGGGAAGATCATTTCTA
TCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCA
AAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGC
ATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATCATTCGCGGTGTAAATGTCTTCCCGTCACAACTGGAAGAAGA
GATTGTCAAATTCGAACATTTATCGCCCCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGA
AAGTGGAGTTGAAAGAAAGCAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTGTGCCACCAGCTGCGCCATCGGATT
AAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCG
GGTGTTTGATCTGCGCAATATTGTTGGTGCCTGA

Upstream 100 bases:

>100_bases
ACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATAT
CAACCTGCGAGTACCCTACA

Downstream 100 bases:

>100_bases
CGTGTCGTCTGGCCCTGGTGAGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACA
ATAACAACAGACTGAATCGA

Product: phenylacetyl-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA

Sequences:

>Translated_437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA
>Mature_437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=437, Percent_Identity=100, Blast_Score=909, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAAK_ECOLI (P76085)

Other databases:

- EMBL:   X97452
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64891
- RefSeq:   AP_002025.1
- RefSeq:   NP_415916.1
- ProteinModelPortal:   P76085
- SMR:   P76085
- DIP:   DIP-10430N
- STRING:   P76085
- EnsemblBacteria:   EBESCT00000002455
- EnsemblBacteria:   EBESCT00000016689
- GeneID:   945963
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5218
- KEGG:   eco:b1398
- EchoBASE:   EB3508
- EcoGene:   EG13745
- eggNOG:   COG1541
- GeneTree:   EBGT00050000011815
- HOGENOM:   HBG579597
- OMA:   MHAVDIY
- ProtClustDB:   CLSK891749
- BioCyc:   EcoCyc:G6719-MONOMER
- BioCyc:   MetaCyc:G6719-MONOMER
- Genevestigator:   P76085
- InterPro:   IPR000873
- InterPro:   IPR011880
- TIGRFAMs:   TIGR02155

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.30

Molecular weight: Translated: 48954; Mature: 48954

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHH
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
HHCCCCCHHHHHCCCCCCHHHHCHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHH
LRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
HHHCCCCCCCEEEEEEECHHHCCCCCHHCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
EEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCH
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
HHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
EECCCCCCCEEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SEGKACRVFDLRNIVGA
CCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHH
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
HHCCCCCHHHHHCCCCCCHHHHCHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHH
LRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
HHHCCCCCCCEEEEEEECHHHCCCCCHHCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
EEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCH
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
HHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
EECCCCCCCEEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SEGKACRVFDLRNIVGA
CCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858