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The map label for this gene is paaE

Identifier: 218694943

GI number: 218694943

Start: 1578358

End: 1579563

Strand: Direct

Name: paaE

Synonym: EC55989_1533

Alternate gene names: 218694943

Gene position: 1578358-1579563 (Clockwise)

Preceding gene: 218694942

Following gene: 218694944

Centisome position: 30.62

GC content: 56.3

Gene sequence:

>1206_bases
ATGCGTGAAGCCTTTATCTGTGACGGAATTCGTACGCCGATTGGCCGCTATGGTGGGGCATTATCAGGTGTTCGGGCTGA
CGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGTCTCGATGCGGAATGTATCGATGATGTGATCC
TCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGT
GTTTCCGGCACAACCATTAACCGCTTGTGTGGTTCCGGGCTGGACGCACTGGGGTTTGCCGCACGGGCGATTAAAGCGGG
CGATGGCGATTTGCTGATCGCCGGTGGCGTGGAGTCAATGTCACGGGCACCGTTTGTTATGGGCAAGGCAGCCAGTGCAT
TTTCTCGTCAGGCAGAGATGTTCGATACCACTATTGGCTGGCGATTTGTGAACCCGCTCATGGCTCAACAATTTGGAACT
GACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATATCGCGAGAAGATCAAGATAGTTTTGCGCTACG
CAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGA
AAGGCGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCA
CCATTTCGTGCCAATGGGGTGATTACCGCAGGCAATGCCTCCGGGGTGAATGACGGAGCCGCTGCGTTGATTATTGCCAG
TGAACAGATGGCAGCAGCGCAAGGACTGACACCGCGGGCGCGAATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCC
TGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCGGGGCTGAGTATTCACGATATGGACGTGATT
GAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAA
CCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGC
TGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGT
GTTTGA

Upstream 100 bases:

>100_bases
GTGTTTACGACATCGAAATTGTTAACCAACAACAAAAAACGGTTGCGCTGTTTCGCGGTAAATCTCACCGCATCGGCGGC
ACCATTACAGGAGAAGCCTG

Downstream 100 bases:

>100_bases
GCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTA
CAGGCGTTGCAAACACAACG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRLDAECIDDVILGCANQAGEDNRNVARMATLLAGLPQS
VSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLRGLKA
PFRANGVITAGNASGVNDGAAALIIASEQMAAAQGLTPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVI
ELNEAFAAQALGVLRELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQGIAMILER
V

Sequences:

>Translated_401_residues
MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRLDAECIDDVILGCANQAGEDNRNVARMATLLAGLPQS
VSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLRGLKA
PFRANGVITAGNASGVNDGAAALIIASEQMAAAQGLTPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVI
ELNEAFAAQALGVLRELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQGIAMILER
V
>Mature_401_residues
MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRLDAECIDDVILGCANQAGEDNRNVARMATLLAGLPQS
VSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLRGLKA
PFRANGVITAGNASGVNDGAAALIIASEQMAAAQGLTPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVI
ELNEAFAAQALGVLRELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQGIAMILER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=43.640897755611, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=40.2010050251256, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI4501853, Length=406, Percent_Identity=40.1477832512315, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=35.3233830845771, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=33.1786542923434, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=30.0751879699248, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=99.7506234413965, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=45.4320987654321, Blast_Score=317, Evalue=6e-88,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=45.5445544554455, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=44.25, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=43.1077694235589, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=36.81592039801, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17535921, Length=408, Percent_Identity=34.3137254901961, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=31.1059907834101, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=30.3703703703704, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17537653, Length=327, Percent_Identity=22.3241590214067, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=37.6237623762376, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=34.5588235294118, Blast_Score=213, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.139650872818, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=41.6873449131514, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=33.6633663366337, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=30.7511737089202, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19921506, Length=314, Percent_Identity=27.0700636942675, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585051, Length=311, Percent_Identity=26.6881028938907, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42247; Mature: 42247

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRLDAECIDDVILGCANQA
CCCCEECCCHHCCHHHHCCCCCCCCCCCCHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGTDSMPETAENVAELLKISRED
HCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLRGLKA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCC
PFRANGVITAGNASGVNDGAAALIIASEQMAAAQGLTPRARIVAMATAGVEPRLMGLGPV
CCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHCCCCCHHEEEEEEECCCCCEEEECCCC
PATRRVLERAGLSIHDMDVIELNEAFAAQALGVLRELGLPDDAPHVNPNGGAIALGHPLG
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLALAASHELHRRNGRYALCTMCIGVGQGIAMILERV
CCCCEEEEHHHHHHHHCCCCEEEHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRLDAECIDDVILGCANQA
CCCCEECCCHHCCHHHHCCCCCCCCCCCCHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGTDSMPETAENVAELLKISRED
HCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLRGLKA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCC
PFRANGVITAGNASGVNDGAAALIIASEQMAAAQGLTPRARIVAMATAGVEPRLMGLGPV
CCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHCCCCCHHEEEEEEECCCCCEEEECCCC
PATRRVLERAGLSIHDMDVIELNEAFAAQALGVLRELGLPDDAPHVNPNGGAIALGHPLG
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLALAASHELHRRNGRYALCTMCIGVGQGIAMILERV
CCCCEEEEHHHHHHHHCCCCEEEHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]