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The map label for this gene is puuP

Identifier: 218694872

GI number: 218694872

Start: 1499528

End: 1500913

Strand: Reverse

Name: puuP

Synonym: EC55989_1458

Alternate gene names: 218694872

Gene position: 1500913-1499528 (Counterclockwise)

Preceding gene: 218694873

Following gene: 218694871

Centisome position: 29.12

GC content: 53.25

Gene sequence:

>1386_bases
ATGGCTATTAATTCACCACTGAATATTGCTGCGCAACCCGGCAAAACCCGTCTGCGAAAATCACTGAAATTGTGGCAGGT
GGTAATGATGGGTCTGGCCTATCTCACGCCGATGACCGTATTTGATACTTTTGGTATTGTCTCCGGCATTAGCGACGGTC
ACGTTCCGGCGTCCTATTTGCTGGCGCTGGCGGGCGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTT
CCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAATCCGCACGTCGGATTTATGGTCGGCTGGTCATCACT
GCTGGATTATCTCTTTTTGCCAATGATCAACGTTTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGC
CGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAACTTCAAT
ACCCTGTTTGTACTGGTGCAAATCTCCATCATGGTGGTGTTTATCTTCCTGGTAGTTCAGGGACTGCATAAAGGAGAAGG
CGTTGGCACCGTCTGGTCACTTCAGCCGTTTATCAGCGAGAACGCGCACCTGATCCCGATTATTACCGGGGCGACGATTG
TCTGTTTCTCGTTCCTCGGTTTCGATGCGGTGACCACGCTTTCGGAAGAGACGCCGGACGCCGCACGCGTGATCCCGAAA
GCCATCTTCCTGACGGCGGTATACGGCGGCGTTATCTTTATCGCGGCGTCGTTCTTTATGCAGCTGTTCTTTCCCGATAT
CAGCCGCTTTAAAGACCCGGACGCCGCACTGCCTGAAATTGCGCTGTACGTCGGCGGCAAGCTCTTCCAGTCGATTTTCC
TCTGCACCACGTTTGTGAACACGTTAGCCTCTGGCCTGGCTTCACATGCCAGCGTGTCGCGTCTGCTGTATGTGATGGGG
CGTGACAATGTGTTTCCGGAGCGCGTGTTTGGCTATGTGCACCCAAAATGGCGGACTCCGGCACTCAACGTCATTATGGT
CGGGATTGTCGCGTTGTCGGCGCTGTTCTTCGATTTAGTTACCGCCACAGCGTTAATTAACTTCGGTGCTCTGGTGGCGT
TTACCTTCGTGAATCTGTCGGTGTTTAATCATTTCTGGCGGCGTAAAGGAATGAATAAAAGCTGGAAGGATCACTTCCAC
TATTTGCTGATGCCGCTGGTTGGCGCGCTGACGGTGGGTGTGCTGTGGGTTAACCTCGAGTCAACGTCACTGACACTCGG
TCTGGTATGGGCTTCGTTAGGCGGCGCATATTTATGGTATCTGATCCGCCGCTATCGCAAAGTGCCGTTGTATGATGGTG
ACAGAACGCCGGTGAGTGAAACGTAA

Upstream 100 bases:

>100_bases
GCTACTGACTGGACTCCGCCCCCTTTGCTTCCCTTGCTAAACAGGTTTGATTTCAGGTTGACACGACCGCAAACCTTATT
ACGCAGGGGAGGCAGCAATT

Downstream 100 bases:

>100_bases
TAGTCGGATGCGCGGCGCATTATCCTGCCTGAGGGTAACGCGCCGCGCCATTAACTATAAAAAGATGTATGACCCGGATC
ACGAGTGCTATCATCCGCCC

Product: putrescine importer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET

Sequences:

>Translated_461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
>Mature_460_residues
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFP
EAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNT
LFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPKA
IFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGR
DNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHY
LLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET

Specific function: Imports putrescine

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Homo sapiens, GI114326544, Length=425, Percent_Identity=22.8235294117647, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI114326550, Length=425, Percent_Identity=22.8235294117647, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4507047, Length=415, Percent_Identity=22.4096385542169, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI258614005, Length=412, Percent_Identity=23.0582524271845, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI258614003, Length=412, Percent_Identity=23.0582524271845, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI87081869, Length=461, Percent_Identity=100, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=446, Percent_Identity=63.677130044843, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1788480, Length=395, Percent_Identity=24.3037974683544, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081708, Length=467, Percent_Identity=24.4111349036403, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786789, Length=175, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786602, Length=176, Percent_Identity=25.5681818181818, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082250, Length=403, Percent_Identity=22.8287841191067, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786302, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=408, Percent_Identity=23.7745098039216, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17533459, Length=404, Percent_Identity=24.5049504950495, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=459, Percent_Identity=24.6187363834423, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=25.8620689655172, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24668806, Length=418, Percent_Identity=24.8803827751196, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356285, Length=418, Percent_Identity=24.8803827751196, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24668802, Length=418, Percent_Identity=24.8803827751196, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281366235, Length=411, Percent_Identity=24.5742092457421, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI116007820, Length=411, Percent_Identity=24.5742092457421, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI116007818, Length=412, Percent_Identity=24.5145631067961, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI85725152, Length=412, Percent_Identity=24.5145631067961, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221512776, Length=396, Percent_Identity=20.959595959596, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666159, Length=396, Percent_Identity=20.959595959596, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161077963, Length=399, Percent_Identity=22.3057644110276, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUUP_ECOLI (P76037)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64878
- RefSeq:   AP_001922.1
- RefSeq:   NP_415812.4
- ProteinModelPortal:   P76037
- DIP:   DIP-11601N
- MINT:   MINT-1252906
- STRING:   P76037
- EnsemblBacteria:   EBESCT00000004385
- EnsemblBacteria:   EBESCT00000016096
- GeneID:   946287
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1289
- KEGG:   eco:b1296
- EchoBASE:   EB3666
- EcoGene:   EG13907
- eggNOG:   COG0531
- GeneTree:   EBGT00050000009005
- HOGENOM:   HBG634400
- OMA:   NGHVPAS
- ProtClustDB:   CLSK2459753
- BioCyc:   EcoCyc:B1296-MONOMER
- Genevestigator:   P76037
- InterPro:   IPR004841
- InterPro:   IPR002293
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 50854; Mature: 50722

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x104cee2c)-; HASH(0x196fbf4c)-; HASH(0x192aaf10)-; HASH(0x1971de48)-; HASH(0x1971b178)-; HASH(0x18687e44)-; HASH(0x104d33cc)-; HASH(0x195a4bd8)-; HASH(0x194d1320)-; HASH(0x162be44c)-; HASH(0x1920dc08)-; HASH(0x1933e8f0)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure 
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503