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The map label for this gene is paaF [C]

Identifier: 218692592

GI number: 218692592

Start: 4990971

End: 4991747

Strand: Reverse

Name: paaF [C]

Synonym: ECED1_5059

Alternate gene names: 218692592

Gene position: 4991747-4990971 (Counterclockwise)

Preceding gene: 218692593

Following gene: 218692591

Centisome position: 95.82

GC content: 49.29

Gene sequence:

>777_bases
ATGAGTGACCAACCCGTTCTGTTCAGCAGAGCGGCGGCTTCTTGCCGCCTGACATTGAACCGTGAAGATAAATGTCATGC
TATTAATGAAGAAATGATCGAATCTCTCGACCATTATTTAAATGAAATTGAAAATGACACGGCATTACGCTTAGTCGAAT
TAACGGCAACCGGCGATAAATTCTTTTGTGCGGGCGGCGATATTAAATCGTGGTCGGCCTATTCGCCATTAGATATGGGC
AGAAAATGGATTAAATGCGGTAATGACGTTTTTAATCGCCTGCGCAACTTACCCCAATTAACCGTTGCGAATCTCAACGG
TCATACCATCGGTGGCGGTATTGAACTTGCCTTGTGCTGCGATATTCGTATCGCTCGCCCCGGCGCGAAATTCTCTAACC
CGGAAGTGATGCTCGGCATGGTGCCAGGCTGGATGGGCATTGAGCGAGTGCTTAATCAGGTTGGTCCTGTGGTCGGTCGC
CAGATGCTGATGCTGGGTAAACGCCTGACGGCGCAGGAAGCTCAGACAGCGAATTTAATTGATGAAGTGGTAGAAAAAGA
ACAGGTGGAAAGCTGGATGGCGAACCAGTTAGCGCAGCTGGAAAAATGTGGTCCGGTGGCACTGGCACACATTAAACAAC
TGATTCTGACGCTGGAAAATAAACACGCCGATTACTCGCATCAGTTGCTGGCTGGATTAATGTCCGCCACTCAGGATTGC
CAGCAGGCGACGCGCGCATTTGCCGAAAAAGCCGGCGTGAGCTTCCACAATCAATAA

Upstream 100 bases:

>100_bases
TGAACTGGAAATATTCTCACCGTACTGGTGGCAGAAAGATATTAACAGCACGCTGGATATTAGCGTCGATCGCATCGCAC
ATTATTGTTAAGGAATAATC

Downstream 100 bases:

>100_bases
GGAAATTGTATGCGTAAGTTATCAACCGCCGAGACACTGGCGGCGCAAATTCAGGACGGCGCAACCATTGCCATTAGCGG
TAACGGCGGCGGCATGGTGG

Product: putative acyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKCGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTLENKHADYSHQLLAGLMSATQDC
QQATRAFAEKAGVSFHNQ

Sequences:

>Translated_258_residues
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKCGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTLENKHADYSHQLLAGLMSATQDC
QQATRAFAEKAGVSFHNQ
>Mature_257_residues
SDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGR
KWIKCGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQ
MLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTLENKHADYSHQLLAGLMSATQDCQ
QATRAFAEKAGVSFHNQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.9212598425197, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI20127408, Length=164, Percent_Identity=29.2682926829268, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=27.5303643724696, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=224, Percent_Identity=29.0178571428571, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI62530384, Length=192, Percent_Identity=28.125, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=24.896265560166, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=27.906976744186, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=28.8135593220339, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=24.5967741935484, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788682, Length=164, Percent_Identity=27.4390243902439, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.5882352941176, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=26.8398268398268, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=25.4166666666667, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=184, Percent_Identity=26.6304347826087, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=24.3027888446215, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=129, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=129, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=129, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=29.6875, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=29.6875, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653139, Length=241, Percent_Identity=25.3112033195021, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571730, Length=191, Percent_Identity=25.6544502617801, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571729, Length=191, Percent_Identity=25.6544502617801, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583165, Length=201, Percent_Identity=29.8507462686567, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921018, Length=222, Percent_Identity=28.8288288288288, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28506; Mature: 28375

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTALRLVELTATGDK
CCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKCGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQTANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTLENKHADYSHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QQATRAFAEKAGVSFHNQ
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTALRLVELTATGDK
CCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKCGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQTANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTLENKHADYSHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QQATRAFAEKAGVSFHNQ
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA