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The map label for this gene is intS [H]

Identifier: 218692579

GI number: 218692579

Start: 4978645

End: 4979826

Strand: Reverse

Name: intS [H]

Synonym: ECED1_5046

Alternate gene names: 218692579

Gene position: 4979826-4978645 (Counterclockwise)

Preceding gene: 218692582

Following gene: 218692578

Centisome position: 95.59

GC content: 44.08

Gene sequence:

>1182_bases
ATGCCACTTACTGTTCGGCAAATTGAGTCGGCCAAACCTGCTGACAAGGATTACAAACTTTCTGATGGTCAAGGACTTTA
CCTTCTTATCAAGAAATCTGGCGCTCGTTACTGGAATCTGAAGTATCGATTTGCGGGTAAAGAAAAGAAATTGGTGATTG
GTGTCTATCCAGGTATATCACTGGCTCTGGCGCGTCAGAAACGGGATGAAGCACGTAAACTCCTCGCTGAAGGAAAAGAT
CCTTCATATGAAAAACAGGCGAGTAAACATGCACTAAGAGAAAGTATTGCATGCACATTTTGTACTCTTGCAGAAGAGTT
GTTCACACATATGGCTTGTCGTTGGACTGCGGGTTATTTAGCGGACCAGCGTGCAAGATTCGAGAATCATATTAATCCCA
TGCTAGGGAAACGTCCTGTGGCTGATATTCGCCCCTTGGAAATATTGAATGCACTGAGGGTTATAGAAAAGAATGGCAAA
CTTGAAGCGTTACGTAAGACTCGTCAGCTCTGTTCTCAAATATTTGATTACGCCATTGCGACGGGGCGCGCCACTATTAA
CCCAGCATCGACTCTCAGCAGTGTTGTTCTTCATCCAGAAAAACAGCAGAGACGGATGCTATCTGAAGAACAGTTAGGCG
CTTTCATGTACCAAATGAAAGTTTCAGGCAATTTTATGTCTCTGGTAACGCAAATACTGGTTCTAACTGCTGTTAGAACG
GGAGAATTACGCCTTGCTACTTGGGATGAAATTGATTTTACCAATGCCTTATGGGTAATCCCAAAAGAGAGAATGAAAAT
GCGAAGGCCGCATGTTGTTCCACTTTCTCATCAGACTCAAATGTTATTGAAAGAGATAAAAATAATCACAGCTCCCCGTA
ACTCCGAGCTGGTTATTCCTGGTGTATACAGAGATAATCAACCTCGTGGTCGCGGTTGCATCAACGAGTTTCTTCAACAA
GTTGGCTGGCATACATTCACTACAGCTCATGGTTTTCGGCATACTTTCAGTACCATTCTCCATGACCATGATTTCAGCTC
TGCCTGGATTGAACTCCAGCTTGCCCATGTTGATAAAAACATCATGCGCGGCACCTACAACCACGCACAGTATCTCGATG
GTCGTCGTGAAATGATGCAATGGTACGCTGACTACATTGATTCACTTTCTGAGCAGGCCTGA

Upstream 100 bases:

>100_bases
TATTGTCATAAATTCTTTTACTCTGGACGTAAACCATCCTCCATGATCATCAATATAATTTTGGGGGCTACTCTGGGGGC
TACTTTTCGGAGTTAACATC

Downstream 100 bases:

>100_bases
TGAATGAAAGTTCAGTGATGCAGAAATAATGAATAACCATCCGGCTTTGGAGGCCGGATGGTTTTATTTATGTTTTCTTT
TTTATTTGCTCACATCTTCT

Product: putative prophage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRFAGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKD
PSYEKQASKHALRESIACTFCTLAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
LEALRKTRQLCSQIFDYAIATGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQMKVSGNFMSLVTQILVLTAVRT
GELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLSHQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQ
VGWHTFTTAHGFRHTFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQA

Sequences:

>Translated_393_residues
MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRFAGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKD
PSYEKQASKHALRESIACTFCTLAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
LEALRKTRQLCSQIFDYAIATGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQMKVSGNFMSLVTQILVLTAVRT
GELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLSHQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQ
VGWHTFTTAHGFRHTFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQA
>Mature_392_residues
PLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRFAGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKDP
SYEKQASKHALRESIACTFCTLAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGKL
EALRKTRQLCSQIFDYAIATGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQMKVSGNFMSLVTQILVLTAVRTG
ELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLSHQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQV
GWHTFTTAHGFRHTFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=391, Percent_Identity=40.6649616368286, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI145693166, Length=392, Percent_Identity=37.7551020408163, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788974, Length=391, Percent_Identity=34.5268542199489, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45177; Mature: 45046

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRFAGKEKKLVIGVYPGIS
CCCCHHHHHCCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEEECCCCCEEEEEECCCHH
LALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCTLAEELFTHMACRWTAGYL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGKLEALRKTRQLCSQIFDYAIA
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQMKVSGNFMSLVTQILVLTAVRT
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCC
GELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLSHQTQMLLKEIKIITAPRNSELVIP
CCEEEEECCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEC
GVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRHTFSTILHDHDFSSAWIELQLAHVDKN
CCCCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHCHH
IMRGTYNHAQYLDGRREMMQWYADYIDSLSEQA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRFAGKEKKLVIGVYPGIS
CCCHHHHHCCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEEECCCCCEEEEEECCCHH
LALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCTLAEELFTHMACRWTAGYL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGKLEALRKTRQLCSQIFDYAIA
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQMKVSGNFMSLVTQILVLTAVRT
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCC
GELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLSHQTQMLLKEIKIITAPRNSELVIP
CCEEEEECCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEC
GVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRHTFSTILHDHDFSSAWIELQLAHVDKN
CCCCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHCHH
IMRGTYNHAQYLDGRREMMQWYADYIDSLSEQA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]