Click here to switch to the map view.

The map label for this gene is mak [H]

Identifier: 218691940

GI number: 218691940

Start: 4265818

End: 4266783

Strand: Reverse

Name: mak [H]

Synonym: ECED1_4342

Alternate gene names: 218691940

Gene position: 4266783-4265818 (Counterclockwise)

Preceding gene: 218691941

Following gene: 218691939

Centisome position: 81.9

GC content: 53.0

Gene sequence:

>966_bases
GTGACCCGCCTTTTACGCAACAAGGAACGAGATATGCACTATCTGGGGTTGGATATTGGTGGGACCAAAATCGCCGCCGT
TGTCATGGATGCGCATGGCTGGGAGATTCGCCGTTACCGCTGCCCGACGCAAAAGTCAACATATCAACAATTTGTCTCAT
GCGTTGTGGCGCTTATCGAGCAGATTAGGCGGGACGTTCAACGACCGATGCTGACAGGGATCGCCTTACCCGGCAGTATC
TCGCCACTCACTGGCCTGATTAAAAACGCGAATATTCAGGTGATTAACGGTCATGCGTTACAGGCTGATTTGCAGCAATT
GCTTGGGCAACCGGTGGTGATAGCCAATGATGGTAACTGTTTCGCGCTATCAGAAGCTTGCGACGGTGCCGGGCAAGATT
ATGACGTGGTATTTGGTATTACGCTTGGTACGGGCTGCGGCGGTGGCATTGCCATCAAGCAACGACCGTTTATAGGGGCC
TGGGGAAATGCTGCCGAATGCGGTCATATCACGTTGCCAGGCTATACGGAGCAGGAAGATGGTCCATCAGTCAGTTGCTA
TTGCGGCAAACACAACTGCGTGGAGTCGTTTGTTTCCGGCAGCGGTTTTAGTGAACGCTATCAACAGATGACGGGTAACT
TGCTCACTTCTGCGGCGATTGTCACCCTGGCACAACGTGGTGATGCTTGTGCCATGCAGCAGGTGGCACGTTTTCGCCAA
CAGCTTGCCCGCACGCTGGCAACCATCGTTAATGTTGTTGACCCTGGCGTGATTGTGATCGGCGGCGGGCTTTCGAATGT
GGAACTGCTTATCACCGATCTGAACGCAGAAGTCGCTCCACTGGTTTTCACCGATCAATTCACCACTCCCATTGTAAAAG
CACAGCACGGCGACAGTAGCGGAATGCGTGGCGCTGCCTGGCTTGCTATGCGCAACGGAGAAGCCAATGAAACGTTCACA
AATTAA

Upstream 100 bases:

>100_bases
ATCCGGATGCGATTAACGCGGCGCGGGAAGTCATTCGCCACAAAATGAAACTGTTCGGTTCGTTCGGTAAAGCATCACTG
TACTAAGCCAGAAGGGCGGG

Downstream 100 bases:

>100_bases
TTATGTTATTGATAAAGCACATGCCATTGCGCAGACCTTCCGCGTTTGTTTGCCAGAGTTCGCCTATTTTACGGTGGATG
ACTGGCTGCAGCGGGAACGG

Product: putative sugar kinase/transcriptional regulator. ATPase domain

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTRLLRNKERDMHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSI
SPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGA
WGNAAECGHITLPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDACAMQQVARFRQ
QLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQFTTPIVKAQHGDSSGMRGAAWLAMRNGEANETFT
N

Sequences:

>Translated_321_residues
MTRLLRNKERDMHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSI
SPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGA
WGNAAECGHITLPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDACAMQQVARFRQ
QLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQFTTPIVKAQHGDSSGMRGAAWLAMRNGEANETFT
N
>Mature_320_residues
TRLLRNKERDMHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSIS
PLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAW
GNAAECGHITLPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDACAMQQVARFRQQ
LARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQFTTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=273, Percent_Identity=27.8388278388278, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI190014632, Length=273, Percent_Identity=27.8388278388278, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4885285, Length=273, Percent_Identity=27.8388278388278, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI298566325, Length=273, Percent_Identity=27.8388278388278, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI87081733, Length=296, Percent_Identity=41.554054054054, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=37.0860927152318, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786891, Length=322, Percent_Identity=24.223602484472, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082230, Length=267, Percent_Identity=30.7116104868914, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790522, Length=305, Percent_Identity=26.2295081967213, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787878, Length=321, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 34361; Mature: 34229

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLRNKERDMHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIE
CCCHHHCCCCCEEEEEEEECCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
QIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNC
HHHHHHHHHHHHCEECCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEEECCCCE
FALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHITLPGYTEQED
EEEHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCEEECCCCCCCCC
GPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDACAMQQVARFRQ
CCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQFTTPIVKAQHGDSS
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCCC
GMRGAAWLAMRNGEANETFTN
CCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TRLLRNKERDMHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIE
CCHHHCCCCCEEEEEEEECCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
QIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNC
HHHHHHHHHHHHCEECCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEEECCCCE
FALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHITLPGYTEQED
EEEHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCEEECCCCCCCCC
GPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDACAMQQVARFRQ
CCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQFTTPIVKAQHGDSS
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCCC
GMRGAAWLAMRNGEANETFTN
CCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]