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The map label for this gene is ydfI [H]

Identifier: 218691309

GI number: 218691309

Start: 3594356

End: 3595828

Strand: Direct

Name: ydfI [H]

Synonym: ECED1_3669

Alternate gene names: 218691309

Gene position: 3594356-3595828 (Clockwise)

Preceding gene: 218691308

Following gene: 218691310

Centisome position: 69.0

GC content: 47.93

Gene sequence:

>1473_bases
ATGTCAGGGAACGAAAGTATCGATGCCGCCCACTATGATCAGGTAACCACCTGGCCTCGGGACGAGTTACAGGCAGATAT
TGTCCATATTGGTTTTGGCGCTTTTCATCGCGGACACCAGGCTGTCTACACGGATCTCACTAATCAGCTTTCGGACACCC
ACTGGGGGATCTTTGAGATCAACCTGTTTGGTGATGCTCAACTGATCGAAAACTTAAATGCGCAAAATGGGCTGTTTTCG
GTTGTGGAAACATCTGCATCGCAATCTACCTCACGCCTTGTGCGTTCGGTAACTGGCGGTATTCATACCCCCGTGGATGG
CATTGCCGCAGCAATCCAGAAATTGACTGAACCTCAGGTAAAAATCGTTTCATTAACCATCACCGAGAAAGGCTATTGTC
TCGATTCGCAAACGCGATCGCTTGATCTCACCAATGGATTAATCAAACACGATCTACAAAACCCGGATGCGCCTCAGTCG
GCTATTGGCTTGATCGTCTGTGCTTTGCAACAACGTAAAGCGGCAGGGCTCGCCGCTTTTAGCGTGCTCTCCTGCGACAA
CTTGCCAGACAATGGGCATCTGACGCGAAATGCCGTTCTCGGTTTTGCCCGACAACTGGACCAGCCCCTGGCTCAGTGGA
TCGAAGAAAATGTCTCATTTCCAGGCACGATGGTAGATCGCATTGTTCCGGCAATGACTGAATCGCAATTCGCCTTACTG
GAAACGAAAACCGGTTATGCCGATCCCTGCGGGATCGTCTGCGAATCATTTCGTCAGTGGGTGATCGAAGATAATTTTGT
GCGCGGACGACCGGAATGGGATAAAGCCGGCGCGATGTTTGTCAGCAATGTTCAGCCTTATGAAGAGATGAAGTTACGCA
TGTTAAATGGTAGCCATTCATTTCTGGCTTATAACGGCTCACTGGCTGGCTATGAATTTATCTGGCAATGCATGGAAGAC
GCTGATTTTCGTTCCATTACCCGCCAACTGATGATTAATGAACAAGCCCGAACACTTAATCCAGACTTAAATATCGACGC
CCAGGAATACGCCGACCTGTTAATTGAACGCTTTAGCAACCGTAATGTTGCACACCGTACCGGGCAAATCGCTATGGACG
GTTCGCAAAAGCTTCCCCAGCGAGCGCTGACGCCCTGGCTGAAATTGCATCAGCAAAAACAAAACAATGCTGTTCTGTCA
CTGCTCGTTGCTGGTTGGTTGCATTATGTCATTGATGCTGTTGAGAAAAGCCAGTCTGTCGCTGATCCAATGAATGACCA
ACTTCAGGCGCTAATAAAGGAACAACAAGACGCATGGCAACAGGCGCTCGCATTACTGCACCTTAGCGCCATATTTGGTG
ATTTAAGCAACCATCAGCCATTTATAAATGAAATAAAAATCGCCTTTGCAAATATAAAAAACAAAGGCATCAAGGCCACC
ATCAGCCAATTATTATCGGATGAGCAGAAATGA

Upstream 100 bases:

>100_bases
CTTTTGCCTTTCTCTGCAAGCGTGATGCATCTCTCTGTTTTTGGTCTAGTGAATTATGTAAATTGGTCTATACACGCCAG
CTACCAGGAGTAAGAGCATA

Downstream 100 bases:

>100_bases
AAACATTAATTTGTCAGCAGCCTGGCGTTATGGAATATGTGGAAAAGGATATTCCCACACCAGCAGATAATGAAGTGCTG
TTAAAAATCAAAGCTGTGGG

Product: putative Fructuronate reductase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYTDLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFS
VVETSASQSTSRLVRSVTGGIHTPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
ETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMED
ADFRSITRQLMINEQARTLNPDLNIDAQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLS
LLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
ISQLLSDEQK

Sequences:

>Translated_490_residues
MSGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYTDLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFS
VVETSASQSTSRLVRSVTGGIHTPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
ETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMED
ADFRSITRQLMINEQARTLNPDLNIDAQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLS
LLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
ISQLLSDEQK
>Mature_489_residues
SGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYTDLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFSV
VETSASQSTSRLVRSVTGGIHTPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSA
IGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALLE
TKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDA
DFRSITRQLMINEQARTLNPDLNIDAQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSL
LVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKATI
SQLLSDEQK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=470, Percent_Identity=49.7872340425532, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1788497, Length=480, Percent_Identity=48.75, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1790779, Length=474, Percent_Identity=49.1561181434599, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI48994885, Length=437, Percent_Identity=24.7139588100687, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790028, Length=281, Percent_Identity=23.4875444839858, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324401, Length=482, Percent_Identity=34.2323651452282, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6320765, Length=482, Percent_Identity=34.2323651452282, Blast_Score=288, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54511; Mature: 54380

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYTDLTNQLSDTHWGIFEI
CCCCCCCCCHHCCCCCCCCHHHHHHHHHEECHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
NLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIHTPVDGIAAAIQKLTEPQV
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCE
KIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSAIGLIVCALQQRKAAGLAAF
EEEEEEEECCCCEECCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
ETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHS
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
FLAYNGSLAGYEFIWQCMEDADFRSITRQLMINEQARTLNPDLNIDAQEYADLLIERFSN
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCC
RNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLHYVIDAVEKSQSV
CCCHHCCCCEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPMNDQLQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
ISQLLSDEQK
HHHHHCCCCC
>Mature Secondary Structure 
SGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYTDLTNQLSDTHWGIFEI
CCCCCCCCHHCCCCCCCCHHHHHHHHHEECHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
NLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIHTPVDGIAAAIQKLTEPQV
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCE
KIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSAIGLIVCALQQRKAAGLAAF
EEEEEEEECCCCEECCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
ETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHS
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
FLAYNGSLAGYEFIWQCMEDADFRSITRQLMINEQARTLNPDLNIDAQEYADLLIERFSN
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCC
RNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLHYVIDAVEKSQSV
CCCHHCCCCEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPMNDQLQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
ISQLLSDEQK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]