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The map label for this gene is yfaU

Identifier: 218690408

GI number: 218690408

Start: 2648175

End: 2648978

Strand: Reverse

Name: yfaU

Synonym: ECED1_2711

Alternate gene names: 218690408

Gene position: 2648978-2648175 (Counterclockwise)

Preceding gene: 218690409

Following gene: 218690402

Centisome position: 50.85

GC content: 53.86

Gene sequence:

>804_bases
ATGAACGCATTATTAACCAATCCCTTTAAAGAACGTTTACGCAAGGGCGAAGTGCAAATTGGTCTGTGGTTAAGCTCAAC
GACTGCCTATATGGCAGAAATTGCCGCCACTTCCGGTTATGACTGGTTGCTGATTGACGGGGAGCATGCGCCAAACACAA
TTCAGGATCTTTATCACCAGCTACAGGCGGTAGCGCCCTATGCCAGCCACCCCGTGATCCGTCCGGTGGAAGGCAGTAAG
CCGCTGATTAAACAAGTCCTGGATATTGGCGCGCAAACTCTACTGATCCCGATGGTCGATACTGCCGATCAGGCACGTCA
GGTGGTGTCTGCCACGCGCTATCCTCCCTACGGTGAGCGTGGTGTTGGGGCCAGTGTGGCACGGGCTGCACGCTGGGGAC
GCATTGAGAATTACATGGCGCAAGTCAACGATTCGCTTTGTCTGTTGGTGCAGGTGGAAAGTAAAACGGCACTGGATAAC
CTGGACGAAATCCTCGATGTCGAAGGGATTGATGGCGTGTTTATTGGACCTGCGGATCTTTCTGCGTCGTTGGGCTACCC
GGATAACGCCGGGCACCCGGAAGTGCAGCGAATTATTGAAACCAGCATTCGGCGGATCCGTGCTGCGGGTAAAGCGGCTG
GTTTTCTGGCTGTGGCTCCTGATATGGCGCAGCAATGCCTGGCGTGGGGAGCGAACTTTGTCGCTGTTGGCGTTGACACG
ATGCTCTACAGCGATGCCCTGGATCAACGACTGGCGATGTTTAAATCAGGCAAAAATGGGCCACGCGTAAAAGGTAGTTA
TTGA

Upstream 100 bases:

>100_bases
CGGTGGCGGTCATCGGTTCGCTGATTATTTTCACTCTGCGTGTAAATCGCACTGTTGCGCAGACCGACGTGGCACATCAT
TAAATAGTTTAAGGAACACG

Downstream 100 bases:

>100_bases
TATCAAAGGCCCATGGGGATCGGCTGTGGGCCTGTGTTAATTAGTGGTTATTCGCTGCCAGATCGGCTTCGCTTAGCTGA
GTGGCCGCGAGCACCTGGTC

Product: putative aldolase

Products: NA

Alternate protein names: KDR aldolase; 2-dehydro-3-deoxyrhamnonate aldolase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSK
PLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT
MLYSDALDQRLAMFKSGKNGPRVKGSY

Sequences:

>Translated_267_residues
MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSK
PLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT
MLYSDALDQRLAMFKSGKNGPRVKGSY
>Mature_267_residues
MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSK
PLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT
MLYSDALDQRLAMFKSGKNGPRVKGSY

Specific function: Catalyzes the reversible retro-aldol cleavage of 2-keto- 3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily

Homologues:

Organism=Escherichia coli, GI1788578, Length=267, Percent_Identity=98.501872659176, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789514, Length=254, Percent_Identity=43.3070866141732, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHMA_ECO45 (B7MG11)

Other databases:

- EMBL:   CU928161
- RefSeq:   YP_002392075.1
- ProteinModelPortal:   B7MG11
- SMR:   B7MG11
- EnsemblBacteria:   EBESCT00000140397
- GeneID:   7133656
- GenomeReviews:   CU928161_GR
- GeneTree:   EBGT00050000008793
- HOGENOM:   HBG747616
- OMA:   SIGSELH
- ProtClustDB:   PRK10128
- BioCyc:   ECOL585035:ECS88_2393-MONOMER
- HAMAP:   MF_01290
- InterPro:   IPR005000
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60

Pfam domain/function: PF03328 HpcH_HpaI; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 28911; Mature: 28911

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: ACT_SITE 49-49 BINDING 151-151 BINDING 178-178 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQ
CCCCCCCHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
LQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGER
HHHHCCCCCCCCEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
GVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDEILDVEGIDGVFIGPADL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCC
SASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEEHHHH
MLYSDALDQRLAMFKSGKNGPRVKGSY
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQ
CCCCCCCHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
LQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGER
HHHHCCCCCCCCEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
GVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDEILDVEGIDGVFIGPADL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCC
SASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEEHHHH
MLYSDALDQRLAMFKSGKNGPRVKGSY
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA