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The map label for this gene is hsdS [H]

Identifier: 218690007

GI number: 218690007

Start: 2218310

End: 2219875

Strand: Reverse

Name: hsdS [H]

Synonym: ECED1_2280

Alternate gene names: 218690007

Gene position: 2219875-2218310 (Counterclockwise)

Preceding gene: 218690008

Following gene: 218690006

Centisome position: 42.61

GC content: 42.08

Gene sequence:

>1566_bases
ATGAATAGAATGACTATTCCCCTTGGTGACATATTAACTCAATCCGGTCACCACCGTGCAGGCAATCGAGAGCTTCCGGT
CCTATCAATAACCATGAAAAATGGTTTGGTTGACCAATCAGACAAATTCAAGAAGCGGATTGCCAGTTCAGACACGTCTA
AATATCGTATTGTATACAAGAATGAACTTGTTGTTGGGTTTCCTATTGACGAGGGAGTTCTTGGATTCCAGACAAAATAT
CCGGTAGGAATCGTTAGCCCAGCTTATGGTATCTGGAAACTCAAGGATGAATCGGTATGCCATATCCCTTACCTTGAGCG
TTATCTGCGCTCCTCCGAGGCAAGGCGACTCTATGCATCTCGTATGCAAGGGGTTGTTGCACGTAGACGTAGCTTGACGA
AGTCTGATTTTCTTAGTTTGGAAGTACCTTTTCCTCCCATCAATGATCAGGCTCGTATAGCTAATTTATTGGCCAAGGTA
GAAGGGCTAATTGAACAGCGTAAACAACTTTTGCAATACCTGGATGACCTACTAAAGAGCGTATTCGTCGATATGTTTAG
TGATCCGGTAAAAAATGCAAAAGGATGGGAACTAACAACAATAGGTGAGTTAGCTGTCGATGTAAGATACGGGACAAGTG
TATCAGCGCAAGGTGGAAAATATAAATACATACGGATGAATAACATTACTCCGGATGGTTACTGGGATTTTGAGAATCTT
AAATATATCGATGTAGATAATAAGGATCTGGATAAATATTCTCTTCAGAAAGGAGATCTCGTTTTCAACCGGACCAATTC
AAAAGAATTGGTTGGTAAAACGGCGGTTTACGATCGAGATGAAACTGTTATTATTGCTGGCTATCTCATTCGTGTTAGGT
TCGATCAACAAACTAACCCTTGGTTTGTATGGGGGCACCTGAACTCAAAGTTTGGAAAAGCAAAATTATTTAATCTTTGT
CGTAATATTATTGGTATGGCTAACATTAATGCCCAAGAGTTAAGGGCCATCCCTATTCTTAAGCCTCCACTGGAGTTACA
AAATAAATTCGCTACTATTGTAGAGAAAGCGCATGCTATTAAATTTCGCTATCAGCAAAGTCTTGCTGATCTTGAGACCC
TGTATGATGTTGTTAGCCAAAAGGCTTTCAAGGGGGAATTAGAACTTTCGCGTGTGCCTATTCCGACACAGATATTTTTC
CCGGTATCAGGTGAAGAACCTGAACACGGGGATGCCGTACAACATATAGCAGTGGGTAATTATCTGCCCGTAACTAATAG
CTTACCATCCGCGTTGGATAATGCAGAGGCTCGCAAGTCACTGATCGAGGAATGGCTAGAGGCCTATATAGCGCAATTGG
GCAACTCATCGTTTTCAGTACATGAGTTCATGATGACGGCGCAGGCACGGCTGGCGGAATTATATCCGGACGTTGATTCT
AAGTTTGGTGAAAGTGACTATGAGCATATCAAAGCTTGGGTGTTCAAAGCTCTGGCTATTGGTACACTGAAGCAGGTATT
CAATGACGCTGACAATTGCATTGAACTTAAGGCGGTTTTGGTATGA

Upstream 100 bases:

>100_bases
GACTAATTCAAGCAGAAGTGGGGAATGTGGATAAGAAAGTGCTTAATAAAGTACAAAGTGGCATCTTGTACGAGCTACTA
GAGTTAAAGGGGATGATAGT

Downstream 100 bases:

>100_bases
AGCTATTACGCCTAAAAATCTCTGATCCGTCCGGCTTCCGTAGTCTGCCCTGTGGTTTTGAACACTATTTTAGAACCGAA
TGGGATTTACAGGAAGAATT

Product: putative type I restriction modification system protein

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFKKRIASSDTSKYRIVYKNELVVGFPIDEGVLGFQTKY
PVGIVSPAYGIWKLKDESVCHIPYLERYLRSSEARRLYASRMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKV
EGLIEQRKQLLQYLDDLLKSVFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQGGKYKYIRMNNITPDGYWDFENL
KYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAVYDRDETVIIAGYLIRVRFDQQTNPWFVWGHLNSKFGKAKLFNLC
RNIIGMANINAQELRAIPILKPPLELQNKFATIVEKAHAIKFRYQQSLADLETLYDVVSQKAFKGELELSRVPIPTQIFF
PVSGEEPEHGDAVQHIAVGNYLPVTNSLPSALDNAEARKSLIEEWLEAYIAQLGNSSFSVHEFMMTAQARLAELYPDVDS
KFGESDYEHIKAWVFKALAIGTLKQVFNDADNCIELKAVLV

Sequences:

>Translated_521_residues
MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFKKRIASSDTSKYRIVYKNELVVGFPIDEGVLGFQTKY
PVGIVSPAYGIWKLKDESVCHIPYLERYLRSSEARRLYASRMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKV
EGLIEQRKQLLQYLDDLLKSVFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQGGKYKYIRMNNITPDGYWDFENL
KYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAVYDRDETVIIAGYLIRVRFDQQTNPWFVWGHLNSKFGKAKLFNLC
RNIIGMANINAQELRAIPILKPPLELQNKFATIVEKAHAIKFRYQQSLADLETLYDVVSQKAFKGELELSRVPIPTQIFF
PVSGEEPEHGDAVQHIAVGNYLPVTNSLPSALDNAEARKSLIEEWLEAYIAQLGNSSFSVHEFMMTAQARLAELYPDVDS
KFGESDYEHIKAWVFKALAIGTLKQVFNDADNCIELKAVLV
>Mature_521_residues
MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFKKRIASSDTSKYRIVYKNELVVGFPIDEGVLGFQTKY
PVGIVSPAYGIWKLKDESVCHIPYLERYLRSSEARRLYASRMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKV
EGLIEQRKQLLQYLDDLLKSVFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQGGKYKYIRMNNITPDGYWDFENL
KYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAVYDRDETVIIAGYLIRVRFDQQTNPWFVWGHLNSKFGKAKLFNLC
RNIIGMANINAQELRAIPILKPPLELQNKFATIVEKAHAIKFRYQQSLADLETLYDVVSQKAFKGELELSRVPIPTQIFF
PVSGEEPEHGDAVQHIAVGNYLPVTNSLPSALDNAEARKSLIEEWLEAYIAQLGNSSFSVHEFMMTAQARLAELYPDVDS
KFGESDYEHIKAWVFKALAIGTLKQVFNDADNCIELKAVLV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=317, Percent_Identity=22.397476340694, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 59077; Mature: 59077

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFKKRIASSDTSKYRIVYK
CCCEECCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
NELVVGFPIDEGVLGFQTKYPVGIVSPAYGIWKLKDESVCHIPYLERYLRSSEARRLYAS
CCEEEEECCCCCCCCCCCCCCCEEECCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHH
RMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKVEGLIEQRKQLLQYLDDLLKS
HHHHHHHHHHHCCHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQGGKYKYIRMNNITPDGYWDFENL
HHHHHHHHHHCCCCCCEEEEEHHEEEEEEECCEECCCCCEEEEEEECCCCCCCCCCCCCE
KYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAVYDRDETVIIAGYLIRVRFDQQTNP
EEEECCCCCCHHHHCCCCCEEEECCCCHHHHHHHHEECCCCEEEEEEEEEEEEECCCCCC
WFVWGHLNSKFGKAKLFNLCRNIIGMANINAQELRAIPILKPPLELQNKFATIVEKAHAI
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
KFRYQQSLADLETLYDVVSQKAFKGELELSRVPIPTQIFFPVSGEEPEHGDAVQHIAVGN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHEECCC
YLPVTNSLPSALDNAEARKSLIEEWLEAYIAQLGNSSFSVHEFMMTAQARLAELYPDVDS
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHH
KFGESDYEHIKAWVFKALAIGTLKQVFNDADNCIELKAVLV
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFKKRIASSDTSKYRIVYK
CCCEECCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
NELVVGFPIDEGVLGFQTKYPVGIVSPAYGIWKLKDESVCHIPYLERYLRSSEARRLYAS
CCEEEEECCCCCCCCCCCCCCCEEECCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHH
RMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKVEGLIEQRKQLLQYLDDLLKS
HHHHHHHHHHHCCHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQGGKYKYIRMNNITPDGYWDFENL
HHHHHHHHHHCCCCCCEEEEEHHEEEEEEECCEECCCCCEEEEEEECCCCCCCCCCCCCE
KYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAVYDRDETVIIAGYLIRVRFDQQTNP
EEEECCCCCCHHHHCCCCCEEEECCCCHHHHHHHHEECCCCEEEEEEEEEEEEECCCCCC
WFVWGHLNSKFGKAKLFNLCRNIIGMANINAQELRAIPILKPPLELQNKFATIVEKAHAI
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
KFRYQQSLADLETLYDVVSQKAFKGELELSRVPIPTQIFFPVSGEEPEHGDAVQHIAVGN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHEECCC
YLPVTNSLPSALDNAEARKSLIEEWLEAYIAQLGNSSFSVHEFMMTAQARLAELYPDVDS
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHH
KFGESDYEHIKAWVFKALAIGTLKQVFNDADNCIELKAVLV
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]