Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 218690008

GI number: 218690008

Start: 2219872

End: 2221545

Strand: Reverse

Name: hsdM [H]

Synonym: ECED1_2281

Alternate gene names: 218690008

Gene position: 2221545-2219872 (Counterclockwise)

Preceding gene: 218690014

Following gene: 218690007

Centisome position: 42.64

GC content: 43.97

Gene sequence:

>1674_bases
ATGCTACAAAACAACCCTGACCTTCAAAGCGCGATCAAACGCCTCTGGGATAAATTCTGGAGTAGCGGCATTAGCAATCC
GCTCACAGCCATTGAACAGATTACCTACCTGTTGTTTATGAAACGGCTAGATGAACTCGATCATAAAAGGCAAGACGAAG
GTGAAACGAGTGGTGAGAAATACATTTCTAAGTTTGCTGGTACTTGGATTCCACCAGAGGAAAGAAACCGCCCTGTTGCG
GAACAACACCCTATAGATAAACGCACGCTTCGCTGGAGCGAGTTCAAGAAATTGCAGCCCGAAGAGATGCTGCAACATGT
GCGAGACAAAGTTTTTCCTTTTCTGAAAGATCTGAATGGTGCTGAATCTAACTTCACTCATCACATGAAAAACGCAGTAT
TCATTATCCCAAAGTCAGCATTGTTGGTGGAGGCAGTGAAGGCCATTGACGAAATCTTCGAGATGATGAAAAGGGATTCT
CAAGAAAAAGGCCAAGCTTTTCAAGACATTCAAGGAGATGTATATGAATTCCTGCTGTCGGAAATCGCCACCGCAGGCAA
GAACGGCCAATTTCGCACACCACGCCACATTATTAAATTGATGGCAGATCTGGTCCAGCCGCAACTGGGACAACGTATTG
CCGACCCTGCCTGCGGTACGGGGGGGTTTCTGTTAGGTGCCTACCAGTACATCCTTACTCAGTTGTCTTTATCTCAAAAC
TTAAAAAGAGATAATTCAAAAGGATCTACTCATGATGAAGATGGGTTCTTTCGCACTTCTGTGACTGCCGCTCTGACCAA
AAAGGCCCGGATTCTTTTGCAAGAGAGTCTGTATGGTTATGACATTGACGCTACTATGGTGCGTTTAGGCCTAATGAACT
TAATGATGCATGGAATTGATGAGCCGCATATTGATTATCAGGACACACTAAGCAAGAGCTATAGTGAAGAAACCAAATAT
GACATAGTGCTAGCAAACCCGCCTTTTACTGGCAGTATAGACAGGGGGGATATTAATGAGAATTTGAAACTCTCAACCAC
TAAAACCGAACTTTTGTTTGTTGAGAACATCTATCGTTTGCTGAAAAAGGGCGGTACCGCCTGTGTGATTGTGCCTCAAG
GAGTATTGTTTGGCTCGGGCAAGGCATTCAAAGAACTACGGCAAACTTTAGTTGAGCGCTGCGACCTCAAGGCTGTCATT
ACTCTGCCCAGTGGAGTGTTTAAACCCTACGCTGGAGTAAGTACTGCCATTTTATTGTTCTCCAAAGTTTTCGGGCCGGG
GGATAAAATTTCCAAACCTGCTACCGATTATGTCTGGTTTTATGAGATGTCTTCCGATGGTTACTCATTGGATGACAAAC
GAAATAAGCTTGAAGGCTACGGCGACCTGCAAGACATCATTCAAAAATACCATTCCCGTGATGAGGCGTCTGACACAGAT
CGTACAGCTAAGTGTTTTATGGTGCCAAGAATAGATATTGAAGCGGAGAATTACGATCTTTCGCTGTCTCGCTATAAGGA
AGAAATATTTGAAGAAGTGCAGTACGAACAGCCTGAAGCTATTCTGGAACGACTAATTCAAGCAGAAGTGGGGAATGTGG
ATAAGAAAGTGCTTAATAAAGTACAAAGTGGCATCTTGTACGAGCTACTAGAGTTAAAGGGGATGATAGTATGA

Upstream 100 bases:

>100_bases
CTGGTAAGTTGCCACTTTAAGAAAGGAAAATGTTCTGAAAAAAGAGTAAACTGCCAGCCACAAAGCACCTCAATAGAGAC
TGAACCGAGAACCCAGCCAC

Downstream 100 bases:

>100_bases
ATAGAATGACTATTCCCCTTGGTGACATATTAACTCAATCCGGTCACCACCGTGCAGGCAATCGAGAGCTTCCGGTCCTA
TCAATAACCATGAAAAATGG

Product: putative HsdM; type I restriction modification enzyme methylase subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKRQDEGETSGEKYISKFAGTWIPPEERNRPVA
EQHPIDKRTLRWSEFKKLQPEEMLQHVRDKVFPFLKDLNGAESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDS
QEKGQAFQDIQGDVYEFLLSEIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFLLGAYQYILTQLSLSQN
LKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDIDATMVRLGLMNLMMHGIDEPHIDYQDTLSKSYSEETKY
DIVLANPPFTGSIDRGDINENLKLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKAVI
TLPSGVFKPYAGVSTAILLFSKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNKLEGYGDLQDIIQKYHSRDEASDTD
RTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQYEQPEAILERLIQAEVGNVDKKVLNKVQSGILYELLELKGMIV

Sequences:

>Translated_557_residues
MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKRQDEGETSGEKYISKFAGTWIPPEERNRPVA
EQHPIDKRTLRWSEFKKLQPEEMLQHVRDKVFPFLKDLNGAESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDS
QEKGQAFQDIQGDVYEFLLSEIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFLLGAYQYILTQLSLSQN
LKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDIDATMVRLGLMNLMMHGIDEPHIDYQDTLSKSYSEETKY
DIVLANPPFTGSIDRGDINENLKLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKAVI
TLPSGVFKPYAGVSTAILLFSKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNKLEGYGDLQDIIQKYHSRDEASDTD
RTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQYEQPEAILERLIQAEVGNVDKKVLNKVQSGILYELLELKGMIV
>Mature_557_residues
MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKRQDEGETSGEKYISKFAGTWIPPEERNRPVA
EQHPIDKRTLRWSEFKKLQPEEMLQHVRDKVFPFLKDLNGAESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDS
QEKGQAFQDIQGDVYEFLLSEIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFLLGAYQYILTQLSLSQN
LKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDIDATMVRLGLMNLMMHGIDEPHIDYQDTLSKSYSEETKY
DIVLANPPFTGSIDRGDINENLKLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKAVI
TLPSGVFKPYAGVSTAILLFSKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNKLEGYGDLQDIIQKYHSRDEASDTD
RTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQYEQPEAILERLIQAEVGNVDKKVLNKVQSGILYELLELKGMIV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=452, Percent_Identity=27.4336283185841, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 63382; Mature: 63382

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKRQDEGETSGEK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YISKFAGTWIPPEERNRPVAEQHPIDKRTLRWSEFKKLQPEEMLQHVRDKVFPFLKDLNG
HHHHHHCCCCCCCCCCCCCHHHCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
AESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDSQEKGQAFQDIQGDVYEFLLS
CCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFLLGAYQYILTQLSLSQN
HHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDIDATMVRLGLMNLMMHGID
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
EPHIDYQDTLSKSYSEETKYDIVLANPPFTGSIDRGDINENLKLSTTKTELLFVENIYRL
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHH
LKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKAVITLPSGVFKPYAGVSTAILLF
HHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
SKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNKLEGYGDLQDIIQKYHSRDEASDTD
HHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCC
RTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQYEQPEAILERLIQAEVGNVDKKVLNK
CCEEEEEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
VQSGILYELLELKGMIV
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLDELDHKRQDEGETSGEK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YISKFAGTWIPPEERNRPVAEQHPIDKRTLRWSEFKKLQPEEMLQHVRDKVFPFLKDLNG
HHHHHHCCCCCCCCCCCCCHHHCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
AESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDSQEKGQAFQDIQGDVYEFLLS
CCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPACGTGGFLLGAYQYILTQLSLSQN
HHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQESLYGYDIDATMVRLGLMNLMMHGID
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
EPHIDYQDTLSKSYSEETKYDIVLANPPFTGSIDRGDINENLKLSTTKTELLFVENIYRL
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHH
LKKGGTACVIVPQGVLFGSGKAFKELRQTLVERCDLKAVITLPSGVFKPYAGVSTAILLF
HHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
SKVFGPGDKISKPATDYVWFYEMSSDGYSLDDKRNKLEGYGDLQDIIQKYHSRDEASDTD
HHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCC
RTAKCFMVPRIDIEAENYDLSLSRYKEEIFEEVQYEQPEAILERLIQAEVGNVDKKVLNK
CCEEEEEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
VQSGILYELLELKGMIV
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]